Abstract
Human umbilical vein endothelial cells were modified with polymer chains to display cell-adhesive sites through the ligand-receptor interaction on the cell surface. Both amine ends of a poly(ethylene oxide) (PEO) (M.W.5000) were allowed to react with a cell-adhesive peptide Gly-Arg-Gly-Asp-Ser (GRGDS), which can specifically bind to an integrin receptor. Large cell agglomerates could be readily and soon obtained by adding GRGDS-PEO-GRGDS chains to a cell suspension and subsequently centrifugating cells. The agglomerated cells adhered onto the collagen-coated PS plate and then cells spread and migrated. Eventually, cell shapes changed from agglomerates to a monolayer. Moreover, we could observed that cells tended to attach each other closer than the conventional cell seeding.
| Original language | English |
|---|---|
| Title of host publication | Polymer Preprints, Japan |
| Pages | 2243 |
| Number of pages | 1 |
| Volume | 54 |
| Edition | 1 |
| Publication status | Published - 2005 |
| Event | 54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 2005 May 25 → 2005 May 27 |
Other
| Other | 54th SPSJ Annual Meeting 2005 |
|---|---|
| Country | Japan |
| City | Yokohama |
| Period | 05/5/25 → 05/5/27 |
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Keywords
- Cell adhesion
- HUVEC
- Poly(ethylene oxide) (PEO)
- RGD
- Tissue Engineering
ASJC Scopus subject areas
- Engineering(all)
Cite this
Supra-structured assemblages of cells modified with polymer chains and their application to tissue engineering. / Kawada, Makiko; Takeuchi, Asako; Fujimoto, Keiji.
Polymer Preprints, Japan. Vol. 54 1. ed. 2005. p. 2243.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Supra-structured assemblages of cells modified with polymer chains and their application to tissue engineering
AU - Kawada, Makiko
AU - Takeuchi, Asako
AU - Fujimoto, Keiji
PY - 2005
Y1 - 2005
N2 - Human umbilical vein endothelial cells were modified with polymer chains to display cell-adhesive sites through the ligand-receptor interaction on the cell surface. Both amine ends of a poly(ethylene oxide) (PEO) (M.W.5000) were allowed to react with a cell-adhesive peptide Gly-Arg-Gly-Asp-Ser (GRGDS), which can specifically bind to an integrin receptor. Large cell agglomerates could be readily and soon obtained by adding GRGDS-PEO-GRGDS chains to a cell suspension and subsequently centrifugating cells. The agglomerated cells adhered onto the collagen-coated PS plate and then cells spread and migrated. Eventually, cell shapes changed from agglomerates to a monolayer. Moreover, we could observed that cells tended to attach each other closer than the conventional cell seeding.
AB - Human umbilical vein endothelial cells were modified with polymer chains to display cell-adhesive sites through the ligand-receptor interaction on the cell surface. Both amine ends of a poly(ethylene oxide) (PEO) (M.W.5000) were allowed to react with a cell-adhesive peptide Gly-Arg-Gly-Asp-Ser (GRGDS), which can specifically bind to an integrin receptor. Large cell agglomerates could be readily and soon obtained by adding GRGDS-PEO-GRGDS chains to a cell suspension and subsequently centrifugating cells. The agglomerated cells adhered onto the collagen-coated PS plate and then cells spread and migrated. Eventually, cell shapes changed from agglomerates to a monolayer. Moreover, we could observed that cells tended to attach each other closer than the conventional cell seeding.
KW - Cell adhesion
KW - HUVEC
KW - Poly(ethylene oxide) (PEO)
KW - RGD
KW - Tissue Engineering
UR - http://www.scopus.com/inward/record.url?scp=33645634700&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645634700&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:33645634700
VL - 54
SP - 2243
BT - Polymer Preprints, Japan
ER -