Supra-structured assemblages of cells modified with polymer chains and their application to tissue engineering

Makiko Kawada, Asako Takeuchi, Keiji Fujimoto

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Human umbilical vein endothelial cells were modified with polymer chains to display cell-adhesive sites through the ligand-receptor interaction on the cell surface. Both amine ends of a poly(ethylene oxide) (PEO) (M.W.5000) were allowed to react with a cell-adhesive peptide Gly-Arg-Gly-Asp-Ser (GRGDS), which can specifically bind to an integrin receptor. Large cell agglomerates could be readily and soon obtained by adding GRGDS-PEO-GRGDS chains to a cell suspension and subsequently centrifugating cells. The agglomerated cells adhered onto the collagen-coated PS plate and then cells spread and migrated. Eventually, cell shapes changed from agglomerates to a monolayer. Moreover, we could observed that cells tended to attach each other closer than the conventional cell seeding.

Original languageEnglish
Title of host publicationPolymer Preprints, Japan
Pages2243
Number of pages1
Volume54
Edition1
Publication statusPublished - 2005
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

Fingerprint

Polyethylene oxides
Tissue engineering
Adhesives
Polymers
Endothelial cells
Collagen
Peptides
Amines
Monolayers
Ligands

Keywords

  • Cell adhesion
  • HUVEC
  • Poly(ethylene oxide) (PEO)
  • RGD
  • Tissue Engineering

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kawada, M., Takeuchi, A., & Fujimoto, K. (2005). Supra-structured assemblages of cells modified with polymer chains and their application to tissue engineering. In Polymer Preprints, Japan (1 ed., Vol. 54, pp. 2243)

Supra-structured assemblages of cells modified with polymer chains and their application to tissue engineering. / Kawada, Makiko; Takeuchi, Asako; Fujimoto, Keiji.

Polymer Preprints, Japan. Vol. 54 1. ed. 2005. p. 2243.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kawada, M, Takeuchi, A & Fujimoto, K 2005, Supra-structured assemblages of cells modified with polymer chains and their application to tissue engineering. in Polymer Preprints, Japan. 1 edn, vol. 54, pp. 2243, 54th SPSJ Annual Meeting 2005, Yokohama, Japan, 05/5/25.
Kawada, Makiko ; Takeuchi, Asako ; Fujimoto, Keiji. / Supra-structured assemblages of cells modified with polymer chains and their application to tissue engineering. Polymer Preprints, Japan. Vol. 54 1. ed. 2005. pp. 2243
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