Supra-structured assemblages of cells modified with polymer chains and their application to tissue engineering

Makiko Kawada, Asako Takeuchi, Keiji Fujimoto

Research output: Contribution to conferencePaperpeer-review

Abstract

Human umbilical vein endothelial cells were modified with polymer chains to display cell-adhesive sites through the ligand-receptor interaction on the cell surface. Both amine ends of a poly(ethylene oxide) (PEO) (M.W.5000) were allowed to react with a cell-adhesive peptide Gly-Arg-Gly-Asp-Ser (GRGDS), which can specifically bind to an integrin receptor. Large cell agglomerates could be readily and soon obtained by adding GRGDS-PEO-GRGDS chains to a cell suspension and subsequently centrifugating cells. The agglomerated cells adhered onto the collagen-coated PS plate and then cells spread and migrated. Eventually, cell shapes changed from agglomerates to a monolayer. Moreover, we could observed that cells tended to attach each other closer than the conventional cell seeding.

Original languageEnglish
Number of pages1
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

Keywords

  • Cell adhesion
  • HUVEC
  • Poly(ethylene oxide) (PEO)
  • RGD
  • Tissue Engineering

ASJC Scopus subject areas

  • Engineering(all)

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