Abstract
Human umbilical vein endothelial cells were modified with polymer chains to display cell-adhesive sites through the ligand-receptor interaction on the cell surface. Both amine ends of a poly(ethylene oxide) (PEO) (M.W.5000) were allowed to react with a cell-adhesive peptide Gly-Arg-Gly-Asp-Ser (GRGDS), which can specifically bind to an integrin receptor. Large cell agglomerates could be readily and soon obtained by adding GRGDS-PEO-GRGDS chains to a cell suspension and subsequently centrifugating cells. The agglomerated cells adhered onto the collagen-coated PS plate and then cells spread and migrated. Eventually, cell shapes changed from agglomerates to a monolayer. Moreover, we could observed that cells tended to attach each other closer than the conventional cell seeding.
Original language | English |
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Number of pages | 1 |
Publication status | Published - 2005 Dec 1 |
Event | 54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 2005 May 25 → 2005 May 27 |
Other
Other | 54th SPSJ Annual Meeting 2005 |
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Country/Territory | Japan |
City | Yokohama |
Period | 05/5/25 → 05/5/27 |
Keywords
- Cell adhesion
- HUVEC
- Poly(ethylene oxide) (PEO)
- RGD
- Tissue Engineering
ASJC Scopus subject areas
- Engineering(all)