Supra-structured assemblages of cells modified with polymer chains and their application to tissue engineering

Makiko Kawada; Asako Takeuchi; Keiji Fujimoto

Supra-structured assemblages of cells modified with polymer chains and their application to tissue engineering

Makiko Kawada, Asako Takeuchi, Keiji Fujimoto

Department of Applied Chemistry

Research output: Contribution to conference › Paper › peer-review

Abstract

Human umbilical vein endothelial cells were modified with polymer chains to display cell-adhesive sites through the ligand-receptor interaction on the cell surface. Both amine ends of a poly(ethylene oxide) (PEO) (M.W.5000) were allowed to react with a cell-adhesive peptide Gly-Arg-Gly-Asp-Ser (GRGDS), which can specifically bind to an integrin receptor. Large cell agglomerates could be readily and soon obtained by adding GRGDS-PEO-GRGDS chains to a cell suspension and subsequently centrifugating cells. The agglomerated cells adhered onto the collagen-coated PS plate and then cells spread and migrated. Eventually, cell shapes changed from agglomerates to a monolayer. Moreover, we could observed that cells tended to attach each other closer than the conventional cell seeding.

Original language	English
Number of pages	1
Publication status	Published - 2005 Dec 1
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 2005 May 25 → 2005 May 27

Other

Other	54th SPSJ Annual Meeting 2005
Country	Japan
City	Yokohama
Period	05/5/25 → 05/5/27

Keywords

Cell adhesion
HUVEC
Poly(ethylene oxide) (PEO)
RGD
Tissue Engineering

ASJC Scopus subject areas

Engineering(all)

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abstract = "Human umbilical vein endothelial cells were modified with polymer chains to display cell-adhesive sites through the ligand-receptor interaction on the cell surface. Both amine ends of a poly(ethylene oxide) (PEO) (M.W.5000) were allowed to react with a cell-adhesive peptide Gly-Arg-Gly-Asp-Ser (GRGDS), which can specifically bind to an integrin receptor. Large cell agglomerates could be readily and soon obtained by adding GRGDS-PEO-GRGDS chains to a cell suspension and subsequently centrifugating cells. The agglomerated cells adhered onto the collagen-coated PS plate and then cells spread and migrated. Eventually, cell shapes changed from agglomerates to a monolayer. Moreover, we could observed that cells tended to attach each other closer than the conventional cell seeding.",

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AU - Kawada, Makiko

AU - Takeuchi, Asako

AU - Fujimoto, Keiji

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N2 - Human umbilical vein endothelial cells were modified with polymer chains to display cell-adhesive sites through the ligand-receptor interaction on the cell surface. Both amine ends of a poly(ethylene oxide) (PEO) (M.W.5000) were allowed to react with a cell-adhesive peptide Gly-Arg-Gly-Asp-Ser (GRGDS), which can specifically bind to an integrin receptor. Large cell agglomerates could be readily and soon obtained by adding GRGDS-PEO-GRGDS chains to a cell suspension and subsequently centrifugating cells. The agglomerated cells adhered onto the collagen-coated PS plate and then cells spread and migrated. Eventually, cell shapes changed from agglomerates to a monolayer. Moreover, we could observed that cells tended to attach each other closer than the conventional cell seeding.

AB - Human umbilical vein endothelial cells were modified with polymer chains to display cell-adhesive sites through the ligand-receptor interaction on the cell surface. Both amine ends of a poly(ethylene oxide) (PEO) (M.W.5000) were allowed to react with a cell-adhesive peptide Gly-Arg-Gly-Asp-Ser (GRGDS), which can specifically bind to an integrin receptor. Large cell agglomerates could be readily and soon obtained by adding GRGDS-PEO-GRGDS chains to a cell suspension and subsequently centrifugating cells. The agglomerated cells adhered onto the collagen-coated PS plate and then cells spread and migrated. Eventually, cell shapes changed from agglomerates to a monolayer. Moreover, we could observed that cells tended to attach each other closer than the conventional cell seeding.

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KW - Poly(ethylene oxide) (PEO)

KW - RGD

KW - Tissue Engineering

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