Surface charge density estimation of vigna mungo protoplasts using a fluorescent dye, 9-aminoacridine

Kotaro Oka, Hiroko Ikeshima, Hideo Ishikawa, Eiji Ohta, Makoto Sakata

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We showed that the surface charge density of protoplasts can be estimated by the 9-aminoacridine method. The estimated surface charge density of the protoplasts isolated from elongating regions of Vigna mungo root was - 39 ± 8 mC/m2. The negative surface charge density increased when protoplasts were treated with glutaraldehyde or when EDTA was added to the protoplast suspension medium. These results support the validity of our estimation of the surface charge density of protoplasts by the 9-aminoacridine method. The concentration of amino groups at the surface of the protoplasts was estimated to be 34 mC/m2.

Original languageEnglish
Pages (from-to)771-775
Number of pages5
JournalPlant and Cell Physiology
Volume29
Issue number5
Publication statusPublished - 1988 Jul

Fingerprint

Aminacrine
Protoplast
Vigna mungo
Protoplasts
fluorescent dyes
Density Estimation
Surface charge
Dyes
Charge density
Fluorescent Dyes
protoplasts
dye
Charge
Ethylenediaminetetraacetic acid
EDTA
glutaraldehyde
Glutaral
Reproducibility of Results
Edetic Acid
Vigna

Keywords

  • 9-Aminoacridine
  • EDTA
  • Glutaraldehyde
  • Surface charge density
  • Vigna mungo (Protoplasts)

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Surface charge density estimation of vigna mungo protoplasts using a fluorescent dye, 9-aminoacridine. / Oka, Kotaro; Ikeshima, Hiroko; Ishikawa, Hideo; Ohta, Eiji; Sakata, Makoto.

In: Plant and Cell Physiology, Vol. 29, No. 5, 07.1988, p. 771-775.

Research output: Contribution to journalArticle

Oka, K, Ikeshima, H, Ishikawa, H, Ohta, E & Sakata, M 1988, 'Surface charge density estimation of vigna mungo protoplasts using a fluorescent dye, 9-aminoacridine', Plant and Cell Physiology, vol. 29, no. 5, pp. 771-775.
Oka, Kotaro ; Ikeshima, Hiroko ; Ishikawa, Hideo ; Ohta, Eiji ; Sakata, Makoto. / Surface charge density estimation of vigna mungo protoplasts using a fluorescent dye, 9-aminoacridine. In: Plant and Cell Physiology. 1988 ; Vol. 29, No. 5. pp. 771-775.
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