Surface charge density estimation of vigna mungo protoplasts using a fluorescent dye, 9-aminoacridine

Kotaro Oka, Hiroko Ikeshima, Hideo Ishikawa, Eiji Ohta, Makoto Sakata

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We showed that the surface charge density of protoplasts can be estimated by the 9-aminoacridine method. The estimated surface charge density of the protoplasts isolated from elongating regions of Vigna mungo root was - 39 ± 8 mC/m2. The negative surface charge density increased when protoplasts were treated with glutaraldehyde or when EDTA was added to the protoplast suspension medium. These results support the validity of our estimation of the surface charge density of protoplasts by the 9-aminoacridine method. The concentration of amino groups at the surface of the protoplasts was estimated to be 34 mC/m2.

Original languageEnglish
Pages (from-to)771-775
Number of pages5
JournalPlant and Cell Physiology
Issue number5
Publication statusPublished - 1988 Jul 1



  • 9-Aminoacridine
  • EDTA
  • Glutaraldehyde
  • Surface charge density
  • Vigna mungo (Protoplasts)

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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