Surfactant protein-B-deficient mice are susceptible to hyperoxic lung injury

Keisuke Tokieda, Harriet S. Iwamoto, Cindy Bachurski, Susan E. Wert, William M. Hull, Kazushige Ikeda, Jeffrey A. Whitsett

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Surfactant protein-B (SP-B) is a small, hydrophobic pcptide that plays a critical role in pulmonary function and surfactant homeostasis. To determine whether SP-B protects mice from oxygen-induced injury, heterozygous SP-B+/- gene-targeted mice and wild-type SP-B+/+ littermates were exposed to hyperoxia (95% oxygen for 3 d) or room air. Although specific lung compliance in room air in SP-B+/- mice was slightly reduced as compared with that in SP-B+/+ mice, it was reduced more markedly during hyperoxia (46% versus 25% decrease, respectively). The larger decrease in lung compliance in SP-B+/- mice was associated with increased severity of pulmonary edema, hemorrhage and inflammation, lung permeability and protein leakage into the alveolar space. Hyperoxia increased SP-B messenger RNA (mRNA) and total protein concentrations by 2-fold in SP-B+/+ and SP-B+/- mice, but decreased the abundance of SP-B protein in lavage fluid relative to total protein only in SP-B+/- mice. Hyperoxia increased SP-B expression, but apparently not enough to maintain SP-B function and lung compliance in the presence of increased protein leakage in SP-B+/- mice. Increased alveolar-capillary leakage and relative deficiency of SP-B may therefore contribute to oxygen-induced pulmonary dysfunction in SP-B+/- mice. These data support the concept that SP-B plays an important protective role in the lung.

Original languageEnglish
Pages (from-to)463-472
Number of pages10
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume21
Issue number4
Publication statusPublished - 1999
Externally publishedYes

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Lung Injury
Surface-Active Agents
Hyperoxia
Lung Compliance
IgA receptor
Oxygen
Proteins
Lung
Air
Pulmonary Surfactants
Therapeutic Irrigation
Pulmonary Edema

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

Tokieda, K., Iwamoto, H. S., Bachurski, C., Wert, S. E., Hull, W. M., Ikeda, K., & Whitsett, J. A. (1999). Surfactant protein-B-deficient mice are susceptible to hyperoxic lung injury. American Journal of Respiratory Cell and Molecular Biology, 21(4), 463-472.

Surfactant protein-B-deficient mice are susceptible to hyperoxic lung injury. / Tokieda, Keisuke; Iwamoto, Harriet S.; Bachurski, Cindy; Wert, Susan E.; Hull, William M.; Ikeda, Kazushige; Whitsett, Jeffrey A.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 21, No. 4, 1999, p. 463-472.

Research output: Contribution to journalArticle

Tokieda, K, Iwamoto, HS, Bachurski, C, Wert, SE, Hull, WM, Ikeda, K & Whitsett, JA 1999, 'Surfactant protein-B-deficient mice are susceptible to hyperoxic lung injury', American Journal of Respiratory Cell and Molecular Biology, vol. 21, no. 4, pp. 463-472.
Tokieda K, Iwamoto HS, Bachurski C, Wert SE, Hull WM, Ikeda K et al. Surfactant protein-B-deficient mice are susceptible to hyperoxic lung injury. American Journal of Respiratory Cell and Molecular Biology. 1999;21(4):463-472.
Tokieda, Keisuke ; Iwamoto, Harriet S. ; Bachurski, Cindy ; Wert, Susan E. ; Hull, William M. ; Ikeda, Kazushige ; Whitsett, Jeffrey A. / Surfactant protein-B-deficient mice are susceptible to hyperoxic lung injury. In: American Journal of Respiratory Cell and Molecular Biology. 1999 ; Vol. 21, No. 4. pp. 463-472.
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