Surgical technique for allogeneic uterus transplantation in macaques

Hideaki Obara, Iori Kisu, Yojiro Kato, Yohei Yamada, Kentaro Matsubara, Katsura Emoto, Masataka Adachi, Yusuke Matoba, Kiyoko Umene, Yuya Nogami, Kouji Banno, Hideaki Tsuchiya, Iori Itagaki, Ikuo Kawamoto, Takahiro Nakagawa, Hirohito Ishigaki, Yasushi Itoh, Kazumasa Ogasawara, Yoko Saiki, Shin Ichi SatoKenshi Nakagawa, Takashi Shiina, Daisuke Aoki, Yuukou Kitagawa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

No study has reported an animal model of uterus transplantation (UTx) using cynomolgus macaques. We aimed to establish a surgical technique of allogeneic UTx assuming the recovery of a uterus from a deceased donor in cynomolgus macaques. Four allogeneic UTxs were performed in female cynomolgus macaques. Donor surgeries comprised en bloc recovery of organs with iliac vessels on both sides, and/or abdominal aorta/vena cava after sufficient perfusion from one femoral artery or external iliac artery. Before perfusion, 150 mL of whole blood was obtained from the donor for subsequent blood transfusion to the recipient. Four uterine grafts were orthotopically transplanted to recipients. End-to-side anastomosis was performed to the iliac vessels on one side in case 1 and iliac vessels on both sides in case 2; aorto-aorto/cavo-caval anastomosis was performed in cases 3 and 4. Arterial blood flow of the uterine grafts was determined by intraoperative indocyanine green (ICG) angiography. ICG angiography results showed sufficient blood flow to all uterine grafts, and anaemia did not progress. Under appropriate immune suppression, all recipients survived for more than 90 days post-transplantation, without any surgical complications. We describe a surgical technique for allogeneic UTx in cynomolgus macaques.

Original languageEnglish
Article number35989
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016 Oct 27

Fingerprint

Homologous Transplantation
Macaca
Uterus
Venae Cavae
Indocyanine Green
Transplants
Angiography
Perfusion
Transplantation
Iliac Artery
Abdominal Aorta
Femoral Artery
Blood Transfusion
Anemia
Animal Models

ASJC Scopus subject areas

  • General

Cite this

Surgical technique for allogeneic uterus transplantation in macaques. / Obara, Hideaki; Kisu, Iori; Kato, Yojiro; Yamada, Yohei; Matsubara, Kentaro; Emoto, Katsura; Adachi, Masataka; Matoba, Yusuke; Umene, Kiyoko; Nogami, Yuya; Banno, Kouji; Tsuchiya, Hideaki; Itagaki, Iori; Kawamoto, Ikuo; Nakagawa, Takahiro; Ishigaki, Hirohito; Itoh, Yasushi; Ogasawara, Kazumasa; Saiki, Yoko; Sato, Shin Ichi; Nakagawa, Kenshi; Shiina, Takashi; Aoki, Daisuke; Kitagawa, Yuukou.

In: Scientific Reports, Vol. 6, 35989, 27.10.2016.

Research output: Contribution to journalArticle

Obara, H, Kisu, I, Kato, Y, Yamada, Y, Matsubara, K, Emoto, K, Adachi, M, Matoba, Y, Umene, K, Nogami, Y, Banno, K, Tsuchiya, H, Itagaki, I, Kawamoto, I, Nakagawa, T, Ishigaki, H, Itoh, Y, Ogasawara, K, Saiki, Y, Sato, SI, Nakagawa, K, Shiina, T, Aoki, D & Kitagawa, Y 2016, 'Surgical technique for allogeneic uterus transplantation in macaques', Scientific Reports, vol. 6, 35989. https://doi.org/10.1038/srep35989
Obara, Hideaki ; Kisu, Iori ; Kato, Yojiro ; Yamada, Yohei ; Matsubara, Kentaro ; Emoto, Katsura ; Adachi, Masataka ; Matoba, Yusuke ; Umene, Kiyoko ; Nogami, Yuya ; Banno, Kouji ; Tsuchiya, Hideaki ; Itagaki, Iori ; Kawamoto, Ikuo ; Nakagawa, Takahiro ; Ishigaki, Hirohito ; Itoh, Yasushi ; Ogasawara, Kazumasa ; Saiki, Yoko ; Sato, Shin Ichi ; Nakagawa, Kenshi ; Shiina, Takashi ; Aoki, Daisuke ; Kitagawa, Yuukou. / Surgical technique for allogeneic uterus transplantation in macaques. In: Scientific Reports. 2016 ; Vol. 6.
@article{31d936da7eb44600be130736647d3e4d,
title = "Surgical technique for allogeneic uterus transplantation in macaques",
abstract = "No study has reported an animal model of uterus transplantation (UTx) using cynomolgus macaques. We aimed to establish a surgical technique of allogeneic UTx assuming the recovery of a uterus from a deceased donor in cynomolgus macaques. Four allogeneic UTxs were performed in female cynomolgus macaques. Donor surgeries comprised en bloc recovery of organs with iliac vessels on both sides, and/or abdominal aorta/vena cava after sufficient perfusion from one femoral artery or external iliac artery. Before perfusion, 150 mL of whole blood was obtained from the donor for subsequent blood transfusion to the recipient. Four uterine grafts were orthotopically transplanted to recipients. End-to-side anastomosis was performed to the iliac vessels on one side in case 1 and iliac vessels on both sides in case 2; aorto-aorto/cavo-caval anastomosis was performed in cases 3 and 4. Arterial blood flow of the uterine grafts was determined by intraoperative indocyanine green (ICG) angiography. ICG angiography results showed sufficient blood flow to all uterine grafts, and anaemia did not progress. Under appropriate immune suppression, all recipients survived for more than 90 days post-transplantation, without any surgical complications. We describe a surgical technique for allogeneic UTx in cynomolgus macaques.",
author = "Hideaki Obara and Iori Kisu and Yojiro Kato and Yohei Yamada and Kentaro Matsubara and Katsura Emoto and Masataka Adachi and Yusuke Matoba and Kiyoko Umene and Yuya Nogami and Kouji Banno and Hideaki Tsuchiya and Iori Itagaki and Ikuo Kawamoto and Takahiro Nakagawa and Hirohito Ishigaki and Yasushi Itoh and Kazumasa Ogasawara and Yoko Saiki and Sato, {Shin Ichi} and Kenshi Nakagawa and Takashi Shiina and Daisuke Aoki and Yuukou Kitagawa",
year = "2016",
month = "10",
day = "27",
doi = "10.1038/srep35989",
language = "English",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Surgical technique for allogeneic uterus transplantation in macaques

AU - Obara, Hideaki

AU - Kisu, Iori

AU - Kato, Yojiro

AU - Yamada, Yohei

AU - Matsubara, Kentaro

AU - Emoto, Katsura

AU - Adachi, Masataka

AU - Matoba, Yusuke

AU - Umene, Kiyoko

AU - Nogami, Yuya

AU - Banno, Kouji

AU - Tsuchiya, Hideaki

AU - Itagaki, Iori

AU - Kawamoto, Ikuo

AU - Nakagawa, Takahiro

AU - Ishigaki, Hirohito

AU - Itoh, Yasushi

AU - Ogasawara, Kazumasa

AU - Saiki, Yoko

AU - Sato, Shin Ichi

AU - Nakagawa, Kenshi

AU - Shiina, Takashi

AU - Aoki, Daisuke

AU - Kitagawa, Yuukou

PY - 2016/10/27

Y1 - 2016/10/27

N2 - No study has reported an animal model of uterus transplantation (UTx) using cynomolgus macaques. We aimed to establish a surgical technique of allogeneic UTx assuming the recovery of a uterus from a deceased donor in cynomolgus macaques. Four allogeneic UTxs were performed in female cynomolgus macaques. Donor surgeries comprised en bloc recovery of organs with iliac vessels on both sides, and/or abdominal aorta/vena cava after sufficient perfusion from one femoral artery or external iliac artery. Before perfusion, 150 mL of whole blood was obtained from the donor for subsequent blood transfusion to the recipient. Four uterine grafts were orthotopically transplanted to recipients. End-to-side anastomosis was performed to the iliac vessels on one side in case 1 and iliac vessels on both sides in case 2; aorto-aorto/cavo-caval anastomosis was performed in cases 3 and 4. Arterial blood flow of the uterine grafts was determined by intraoperative indocyanine green (ICG) angiography. ICG angiography results showed sufficient blood flow to all uterine grafts, and anaemia did not progress. Under appropriate immune suppression, all recipients survived for more than 90 days post-transplantation, without any surgical complications. We describe a surgical technique for allogeneic UTx in cynomolgus macaques.

AB - No study has reported an animal model of uterus transplantation (UTx) using cynomolgus macaques. We aimed to establish a surgical technique of allogeneic UTx assuming the recovery of a uterus from a deceased donor in cynomolgus macaques. Four allogeneic UTxs were performed in female cynomolgus macaques. Donor surgeries comprised en bloc recovery of organs with iliac vessels on both sides, and/or abdominal aorta/vena cava after sufficient perfusion from one femoral artery or external iliac artery. Before perfusion, 150 mL of whole blood was obtained from the donor for subsequent blood transfusion to the recipient. Four uterine grafts were orthotopically transplanted to recipients. End-to-side anastomosis was performed to the iliac vessels on one side in case 1 and iliac vessels on both sides in case 2; aorto-aorto/cavo-caval anastomosis was performed in cases 3 and 4. Arterial blood flow of the uterine grafts was determined by intraoperative indocyanine green (ICG) angiography. ICG angiography results showed sufficient blood flow to all uterine grafts, and anaemia did not progress. Under appropriate immune suppression, all recipients survived for more than 90 days post-transplantation, without any surgical complications. We describe a surgical technique for allogeneic UTx in cynomolgus macaques.

UR - http://www.scopus.com/inward/record.url?scp=84992536728&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84992536728&partnerID=8YFLogxK

U2 - 10.1038/srep35989

DO - 10.1038/srep35989

M3 - Article

C2 - 27786258

AN - SCOPUS:84992536728

VL - 6

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 35989

ER -