Survey for Trypanosoma cruzi infection in a municipality in Northeast Brazil

Hiroshi Tachibana, Kilma C. Paz, W. Hugo W Landivar, Seiki Kobayashi, Luciano T. Montenegro, José Figuerêdo-Silva, Seiki Tateno

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

An epidemiological survey of Trypanosoma cruzi infection was carried out in Bodocó, located in the western part of the State of Pernambuco, Brazil. Two hundred and forty-one individuals were parasitologically and immunologically screened. Although hemoculture did not reveal the presence of parasites in the blood, the sera of 5 individuals were scored as positive by the indirect fluorescence antibody test and the enzyme-linked immunosorbent assay. Seropositivity in individuals above and below the age of 40 was 14.8 and 0.5%, respectively. These results indicate that recent infections with T. cruzi are rare in this area. However, since a T. cruzi-infected triatomid (Triatoma brasiliensis) was captured in a school classroom, this area must be considered endemic. When triatomid feces containing parasites were inoculated into a jird (mongolian gerbil), parasitemia appeared 10 days later. Immunohistochemical staining, using monoclonal antibody specific for T. cruzi, labeled organisms in jird tissues. These observations demonstrate that the jird is a suitable host for experimental T. cruzi infections and that monoclonal antibody is effective for detection of the parasite in host tissues.

Original languageEnglish
Pages (from-to)131-136
Number of pages6
JournalTokai Journal of Experimental and Clinical Medicine
Volume24
Issue number3
Publication statusPublished - 1999 Oct

Fingerprint

Trypanosoma cruzi
Gerbillinae
Brazil
Infection
Parasites
Monoclonal Antibodies
Triatoma
Parasitemia
Feces
Fluorescence
Enzyme-Linked Immunosorbent Assay
Surveys and Questionnaires
Staining and Labeling
Antibodies
Serum

Keywords

  • Immunohistochemistry
  • Jird
  • Monoclonal antibody
  • Serology
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Tachibana, H., Paz, K. C., Landivar, W. H. W., Kobayashi, S., Montenegro, L. T., Figuerêdo-Silva, J., & Tateno, S. (1999). Survey for Trypanosoma cruzi infection in a municipality in Northeast Brazil. Tokai Journal of Experimental and Clinical Medicine, 24(3), 131-136.

Survey for Trypanosoma cruzi infection in a municipality in Northeast Brazil. / Tachibana, Hiroshi; Paz, Kilma C.; Landivar, W. Hugo W; Kobayashi, Seiki; Montenegro, Luciano T.; Figuerêdo-Silva, José; Tateno, Seiki.

In: Tokai Journal of Experimental and Clinical Medicine, Vol. 24, No. 3, 10.1999, p. 131-136.

Research output: Contribution to journalArticle

Tachibana, H, Paz, KC, Landivar, WHW, Kobayashi, S, Montenegro, LT, Figuerêdo-Silva, J & Tateno, S 1999, 'Survey for Trypanosoma cruzi infection in a municipality in Northeast Brazil', Tokai Journal of Experimental and Clinical Medicine, vol. 24, no. 3, pp. 131-136.
Tachibana H, Paz KC, Landivar WHW, Kobayashi S, Montenegro LT, Figuerêdo-Silva J et al. Survey for Trypanosoma cruzi infection in a municipality in Northeast Brazil. Tokai Journal of Experimental and Clinical Medicine. 1999 Oct;24(3):131-136.
Tachibana, Hiroshi ; Paz, Kilma C. ; Landivar, W. Hugo W ; Kobayashi, Seiki ; Montenegro, Luciano T. ; Figuerêdo-Silva, José ; Tateno, Seiki. / Survey for Trypanosoma cruzi infection in a municipality in Northeast Brazil. In: Tokai Journal of Experimental and Clinical Medicine. 1999 ; Vol. 24, No. 3. pp. 131-136.
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