Abstract
Diethyl L-aspartate was polymerized by a bacterial protease from Bacillus subtilis (BS) in organic solvent at a temperature between 30 and 50 °C to yield α-linked poly(ethyl L-aspartate) having an Mw of up to 3700 and a maximum polymer yield of 85%. The best polymerization conditions were the 40 °C polymerization of diethyl L-aspartate using 30% protease BS containing 4.5 vol % water in acetonitrile for 2 days. Poly(ethyl L-aspartate) was readily depolymerized by the enzyme into the oligomeric and monomeric L-aspartate in aqueous acetonitrile. Poly(sodium aspartate) prepared by the saponification of poly(ethyl L-aspartate) was readily biodegradable by activated sludge obtained from the municipal sewage treatment plant. Also, poly(sodium aspartate) was depolymerized by the hydrolase enzyme into the monomeric aspartate. These results may indicate the sustainable chemical recycling and biorecycling of this polymer.
| Original language | English |
|---|---|
| Pages (from-to) | 196-203 |
| Number of pages | 8 |
| Journal | Biomacromolecules |
| Volume | 4 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2003 Mar |
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ASJC Scopus subject areas
- Organic Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Polymers and Plastics
- Materials Chemistry
Cite this
Sustainable enzymatic preparation of polyaspartate using a bacterial protease. / Soeda, Yasuyuki; Toshima, Kazunobu; Matsumura, Shuichi.
In: Biomacromolecules, Vol. 4, No. 2, 03.2003, p. 196-203.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sustainable enzymatic preparation of polyaspartate using a bacterial protease
AU - Soeda, Yasuyuki
AU - Toshima, Kazunobu
AU - Matsumura, Shuichi
PY - 2003/3
Y1 - 2003/3
N2 - Diethyl L-aspartate was polymerized by a bacterial protease from Bacillus subtilis (BS) in organic solvent at a temperature between 30 and 50 °C to yield α-linked poly(ethyl L-aspartate) having an Mw of up to 3700 and a maximum polymer yield of 85%. The best polymerization conditions were the 40 °C polymerization of diethyl L-aspartate using 30% protease BS containing 4.5 vol % water in acetonitrile for 2 days. Poly(ethyl L-aspartate) was readily depolymerized by the enzyme into the oligomeric and monomeric L-aspartate in aqueous acetonitrile. Poly(sodium aspartate) prepared by the saponification of poly(ethyl L-aspartate) was readily biodegradable by activated sludge obtained from the municipal sewage treatment plant. Also, poly(sodium aspartate) was depolymerized by the hydrolase enzyme into the monomeric aspartate. These results may indicate the sustainable chemical recycling and biorecycling of this polymer.
AB - Diethyl L-aspartate was polymerized by a bacterial protease from Bacillus subtilis (BS) in organic solvent at a temperature between 30 and 50 °C to yield α-linked poly(ethyl L-aspartate) having an Mw of up to 3700 and a maximum polymer yield of 85%. The best polymerization conditions were the 40 °C polymerization of diethyl L-aspartate using 30% protease BS containing 4.5 vol % water in acetonitrile for 2 days. Poly(ethyl L-aspartate) was readily depolymerized by the enzyme into the oligomeric and monomeric L-aspartate in aqueous acetonitrile. Poly(sodium aspartate) prepared by the saponification of poly(ethyl L-aspartate) was readily biodegradable by activated sludge obtained from the municipal sewage treatment plant. Also, poly(sodium aspartate) was depolymerized by the hydrolase enzyme into the monomeric aspartate. These results may indicate the sustainable chemical recycling and biorecycling of this polymer.
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UR - http://www.scopus.com/inward/citedby.url?scp=0037738602&partnerID=8YFLogxK
U2 - 10.1021/bm0200534
DO - 10.1021/bm0200534
M3 - Article
C2 - 12625712
AN - SCOPUS:0037738602
VL - 4
SP - 196
EP - 203
JO - Biomacromolecules
JF - Biomacromolecules
SN - 1525-7797
IS - 2
ER -