Sustained Decrease and Remarkable Increase in Red Blood Cell Velocity in Intraparenchymal Capillaries Associated With Potassium-Induced Cortical Spreading Depression

Miyuki Unekawa, Minoru Tomita, Yutaka Tomita, Haruki Toriumi, Norihiro Suzuki

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objectives: To examine changes in red blood cell (RBC) velocity in intraparenchymal capillaries of rat cerebral cortex in response to KCl-induced cortical spreading depression (CSD). Methods: In isoflurane-anesthetized rats, the velocity of fluorescently labeled RBCs flowing in capillaries in layer I was measured with a high-speed camera laser-scanning confocal fluorescence microscope, with simultaneous monitoring of DC potential, the electroencephalogram (EEG), partial pressure of oxygen (PO 2), and cerebral blood flow (CBF). Results: After KCl application, a transient deflection of DC potential (i.e., CSD) repeatedly appeared concomitantly with depression of EEG, and was propagated in the distal direction. PO 2 transiently decreased and CBF was slowly elevated. The frequency distribution of RBC velocity was shifted downward during CSD and was still low after the passage of CSD. When we observed RBC velocity in 38 individual capillaries, 10 capillaries exhibited slowed-down RBC during CSD and RBC velocity remained low in 2 even after the passage of CSD. On the other hand, RBCs with moderately (<3mm/sec) or remarkably (>3mm/sec) increased velocities were seen in 10 and 5 capillaries, respectively. Conclusion: CSD-induced excitation of neurons may sustainably decrease or greatly increase RBC velocity in capillaries.

Original languageEnglish
Pages (from-to)166-174
Number of pages9
JournalMicrocirculation
Volume19
Issue number2
DOIs
Publication statusPublished - 2012 Feb

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Cortical Spreading Depression
Potassium
Erythrocytes
Cerebrovascular Circulation
Electroencephalography
Partial Pressure
Isoflurane
Cerebral Cortex
Lasers
Fluorescence
Oxygen
Neurons

Keywords

  • Confocal fluorescence microscopy
  • Cortical spreading depression
  • Neuro-capillary coupling
  • Red blood cell velocity
  • Thoroughfare channel

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Sustained Decrease and Remarkable Increase in Red Blood Cell Velocity in Intraparenchymal Capillaries Associated With Potassium-Induced Cortical Spreading Depression. / Unekawa, Miyuki; Tomita, Minoru; Tomita, Yutaka; Toriumi, Haruki; Suzuki, Norihiro.

In: Microcirculation, Vol. 19, No. 2, 02.2012, p. 166-174.

Research output: Contribution to journalArticle

Unekawa, Miyuki ; Tomita, Minoru ; Tomita, Yutaka ; Toriumi, Haruki ; Suzuki, Norihiro. / Sustained Decrease and Remarkable Increase in Red Blood Cell Velocity in Intraparenchymal Capillaries Associated With Potassium-Induced Cortical Spreading Depression. In: Microcirculation. 2012 ; Vol. 19, No. 2. pp. 166-174.
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abstract = "Objectives: To examine changes in red blood cell (RBC) velocity in intraparenchymal capillaries of rat cerebral cortex in response to KCl-induced cortical spreading depression (CSD). Methods: In isoflurane-anesthetized rats, the velocity of fluorescently labeled RBCs flowing in capillaries in layer I was measured with a high-speed camera laser-scanning confocal fluorescence microscope, with simultaneous monitoring of DC potential, the electroencephalogram (EEG), partial pressure of oxygen (PO 2), and cerebral blood flow (CBF). Results: After KCl application, a transient deflection of DC potential (i.e., CSD) repeatedly appeared concomitantly with depression of EEG, and was propagated in the distal direction. PO 2 transiently decreased and CBF was slowly elevated. The frequency distribution of RBC velocity was shifted downward during CSD and was still low after the passage of CSD. When we observed RBC velocity in 38 individual capillaries, 10 capillaries exhibited slowed-down RBC during CSD and RBC velocity remained low in 2 even after the passage of CSD. On the other hand, RBCs with moderately (<3mm/sec) or remarkably (>3mm/sec) increased velocities were seen in 10 and 5 capillaries, respectively. Conclusion: CSD-induced excitation of neurons may sustainably decrease or greatly increase RBC velocity in capillaries.",
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