Abstract
Objectives: To examine changes in red blood cell (RBC) velocity in intraparenchymal capillaries of rat cerebral cortex in response to KCl-induced cortical spreading depression (CSD). Methods: In isoflurane-anesthetized rats, the velocity of fluorescently labeled RBCs flowing in capillaries in layer I was measured with a high-speed camera laser-scanning confocal fluorescence microscope, with simultaneous monitoring of DC potential, the electroencephalogram (EEG), partial pressure of oxygen (PO 2), and cerebral blood flow (CBF). Results: After KCl application, a transient deflection of DC potential (i.e., CSD) repeatedly appeared concomitantly with depression of EEG, and was propagated in the distal direction. PO 2 transiently decreased and CBF was slowly elevated. The frequency distribution of RBC velocity was shifted downward during CSD and was still low after the passage of CSD. When we observed RBC velocity in 38 individual capillaries, 10 capillaries exhibited slowed-down RBC during CSD and RBC velocity remained low in 2 even after the passage of CSD. On the other hand, RBCs with moderately (<3mm/sec) or remarkably (>3mm/sec) increased velocities were seen in 10 and 5 capillaries, respectively. Conclusion: CSD-induced excitation of neurons may sustainably decrease or greatly increase RBC velocity in capillaries.
| Original language | English |
|---|---|
| Pages (from-to) | 166-174 |
| Number of pages | 9 |
| Journal | Microcirculation |
| Volume | 19 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2012 Feb |
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Keywords
- Confocal fluorescence microscopy
- Cortical spreading depression
- Neuro-capillary coupling
- Red blood cell velocity
- Thoroughfare channel
ASJC Scopus subject areas
- Physiology
- Physiology (medical)
- Molecular Biology
- Cardiology and Cardiovascular Medicine
Cite this
Sustained Decrease and Remarkable Increase in Red Blood Cell Velocity in Intraparenchymal Capillaries Associated With Potassium-Induced Cortical Spreading Depression. / Unekawa, Miyuki; Tomita, Minoru; Tomita, Yutaka; Toriumi, Haruki; Suzuki, Norihiro.
In: Microcirculation, Vol. 19, No. 2, 02.2012, p. 166-174.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sustained Decrease and Remarkable Increase in Red Blood Cell Velocity in Intraparenchymal Capillaries Associated With Potassium-Induced Cortical Spreading Depression
AU - Unekawa, Miyuki
AU - Tomita, Minoru
AU - Tomita, Yutaka
AU - Toriumi, Haruki
AU - Suzuki, Norihiro
PY - 2012/2
Y1 - 2012/2
N2 - Objectives: To examine changes in red blood cell (RBC) velocity in intraparenchymal capillaries of rat cerebral cortex in response to KCl-induced cortical spreading depression (CSD). Methods: In isoflurane-anesthetized rats, the velocity of fluorescently labeled RBCs flowing in capillaries in layer I was measured with a high-speed camera laser-scanning confocal fluorescence microscope, with simultaneous monitoring of DC potential, the electroencephalogram (EEG), partial pressure of oxygen (PO 2), and cerebral blood flow (CBF). Results: After KCl application, a transient deflection of DC potential (i.e., CSD) repeatedly appeared concomitantly with depression of EEG, and was propagated in the distal direction. PO 2 transiently decreased and CBF was slowly elevated. The frequency distribution of RBC velocity was shifted downward during CSD and was still low after the passage of CSD. When we observed RBC velocity in 38 individual capillaries, 10 capillaries exhibited slowed-down RBC during CSD and RBC velocity remained low in 2 even after the passage of CSD. On the other hand, RBCs with moderately (<3mm/sec) or remarkably (>3mm/sec) increased velocities were seen in 10 and 5 capillaries, respectively. Conclusion: CSD-induced excitation of neurons may sustainably decrease or greatly increase RBC velocity in capillaries.
AB - Objectives: To examine changes in red blood cell (RBC) velocity in intraparenchymal capillaries of rat cerebral cortex in response to KCl-induced cortical spreading depression (CSD). Methods: In isoflurane-anesthetized rats, the velocity of fluorescently labeled RBCs flowing in capillaries in layer I was measured with a high-speed camera laser-scanning confocal fluorescence microscope, with simultaneous monitoring of DC potential, the electroencephalogram (EEG), partial pressure of oxygen (PO 2), and cerebral blood flow (CBF). Results: After KCl application, a transient deflection of DC potential (i.e., CSD) repeatedly appeared concomitantly with depression of EEG, and was propagated in the distal direction. PO 2 transiently decreased and CBF was slowly elevated. The frequency distribution of RBC velocity was shifted downward during CSD and was still low after the passage of CSD. When we observed RBC velocity in 38 individual capillaries, 10 capillaries exhibited slowed-down RBC during CSD and RBC velocity remained low in 2 even after the passage of CSD. On the other hand, RBCs with moderately (<3mm/sec) or remarkably (>3mm/sec) increased velocities were seen in 10 and 5 capillaries, respectively. Conclusion: CSD-induced excitation of neurons may sustainably decrease or greatly increase RBC velocity in capillaries.
KW - Confocal fluorescence microscopy
KW - Cortical spreading depression
KW - Neuro-capillary coupling
KW - Red blood cell velocity
KW - Thoroughfare channel
UR - http://www.scopus.com/inward/record.url?scp=84856888505&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84856888505&partnerID=8YFLogxK
U2 - 10.1111/j.1549-8719.2011.00143.x
DO - 10.1111/j.1549-8719.2011.00143.x
M3 - Article
C2 - 21992739
AN - SCOPUS:84856888505
VL - 19
SP - 166
EP - 174
JO - Microcirculation
JF - Microcirculation
SN - 1073-9688
IS - 2
ER -