Synaptotagmin 2 Is the Fast Ca2+ Sensor at a Central Inhibitory Synapse

Chong Chen, Itaru Arai, Rachel Satterfield, Samuel M. Young, Peter Jonas

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

GABAergic synapses in brain circuits generate inhibitory output signals with submillisecond latency and temporal precision. Whether the molecular identity of the release sensor contributes to these signaling properties remains unclear. Here, we examined the Ca2+ sensor of exocytosis at GABAergic basket cell (BC) to Purkinje cell (PC) synapses in cerebellum. Immunolabeling suggested that BC terminals selectively expressed synaptotagmin 2 (Syt2), whereas synaptotagmin 1 (Syt1) was enriched in excitatory terminals. Genetic elimination of Syt2 reduced action potential-evoked release to ∼10%, identifying Syt2 as the major Ca2+ sensor at BC-PC synapses. Differential adenovirus-mediated rescue revealed that Syt2 triggered release with shorter latency and higher temporal precision and mediated faster vesicle pool replenishment than Syt1. Furthermore, deletion of Syt2 severely reduced and delayed disynaptic inhibition following parallel fiber stimulation. Thus, the selective use of Syt2 as release sensor at BC-PC synapses ensures fast and efficient feedforward inhibition in cerebellar microcircuits.

Original languageEnglish
Pages (from-to)723-736
Number of pages14
JournalCell Reports
Volume18
Issue number3
DOIs
Publication statusPublished - 2017 Jan 17
Externally publishedYes

Fingerprint

Synaptotagmin II
Synapses
Purkinje Cells
Sensors
Synaptotagmin I
Bioelectric potentials
Exocytosis
Adenoviridae
Cerebellum
Action Potentials
Brain
Networks (circuits)
Fibers

Keywords

  • basket cells
  • Ca sensor
  • cerebellum
  • endocytosis
  • exocytosis
  • feedforward inhibition
  • GABAergic synapses
  • pool replenishment
  • synaptotagmin
  • transmitter release

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Synaptotagmin 2 Is the Fast Ca2+ Sensor at a Central Inhibitory Synapse. / Chen, Chong; Arai, Itaru; Satterfield, Rachel; Young, Samuel M.; Jonas, Peter.

In: Cell Reports, Vol. 18, No. 3, 17.01.2017, p. 723-736.

Research output: Contribution to journalArticle

Chen, Chong ; Arai, Itaru ; Satterfield, Rachel ; Young, Samuel M. ; Jonas, Peter. / Synaptotagmin 2 Is the Fast Ca2+ Sensor at a Central Inhibitory Synapse. In: Cell Reports. 2017 ; Vol. 18, No. 3. pp. 723-736.
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