Synaptotagmin IV is present at the Golgi and distal parts of neurites

Keiji Ibata, Mitsunori Fukuda, Tomohiro Hamada, Hiroyuki Kabayama, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Synaptotagmin IV (SytIV) is an immediate early gene induced by membrane depolarization in PC12 cells and in rat brain. However, little is known about the function of SytlV or the functional relationship between SytlV and SytI, because SytlV has yet to be localized. Here we show that SytlV was localized at the Golgi and distal part of neurites in nerve growth factor- differentiated PC12 cells and cultured hippocampal neurons by immunocytochemistry using an isoform-specific antibody (anti-SytIV). These SytlV signals were not colocalized well with SytI signals. Upon membrane depolarization, SytlV signals were increased at both the Golgi and distal part of neurites within several hours in both types of cells. We further show that the increase of SytlV protein levels results from protein kinase A- dependent gene up-regulation. In hippocampal neurons, SytlV was developmentally regulated. These results suggest that SytlV may play a role at the Golgi and tips of neurites during development and synaptic plasticity.

Original languageEnglish
Pages (from-to)518-526
Number of pages9
JournalJournal of Neurochemistry
Volume74
Issue number2
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Synaptotagmins
Depolarization
Neurites
Neurons
Genes
PC12 Cells
Membranes
Nerve Growth Factor
Cyclic AMP-Dependent Protein Kinases
Plasticity
Rats
Anti-Idiotypic Antibodies
Brain
Protein Isoforms
Neuronal Plasticity
Immediate-Early Genes
Up-Regulation
Immunohistochemistry
Proteins

Keywords

  • Depolarization
  • Forskolin
  • Immediate early genes
  • Neuronal development
  • Synaptic plasticity
  • Synaptotagmin

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Synaptotagmin IV is present at the Golgi and distal parts of neurites. / Ibata, Keiji; Fukuda, Mitsunori; Hamada, Tomohiro; Kabayama, Hiroyuki; Mikoshiba, Katsuhiko.

In: Journal of Neurochemistry, Vol. 74, No. 2, 2000, p. 518-526.

Research output: Contribution to journalArticle

Ibata, K, Fukuda, M, Hamada, T, Kabayama, H & Mikoshiba, K 2000, 'Synaptotagmin IV is present at the Golgi and distal parts of neurites', Journal of Neurochemistry, vol. 74, no. 2, pp. 518-526. https://doi.org/10.1046/j.1471-4159.2000.740518.x
Ibata, Keiji ; Fukuda, Mitsunori ; Hamada, Tomohiro ; Kabayama, Hiroyuki ; Mikoshiba, Katsuhiko. / Synaptotagmin IV is present at the Golgi and distal parts of neurites. In: Journal of Neurochemistry. 2000 ; Vol. 74, No. 2. pp. 518-526.
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