TY - JOUR
T1 - Synergistic induction of astrocytic differentiation by factors secreted from meninges in the mouse developing brain
AU - Kawamura, Yoichiro
AU - Katada, Sayako
AU - Noguchi, Hirofumi
AU - Yamamoto, Hiroyuki
AU - Sanosaka, Tsukasa
AU - Iihara, Koji
AU - Nakashima, Kinichi
N1 - Funding Information:
We thank Dr T. Imamura for valuable discussions and helpful comments. We are very grateful to Y. Nakagawa for her excellent secretarial assistance and to A. Sakai, M. Honda, H. Nakashima, K. Irie, and S. Kimura for technical help. We thank Dr T. Kitamura (Tokyo University) for pMY vector and Plat-E cells. This work was supported by grants from JSPS KAKENHI (15K14452) and MEXT KAKENHI (16H06527) to KN and MEXT KAKENHI (17H05647) to SK.
Funding Information:
We thank Dr T. Imamura for valuable discussions and helpful comments. We are very grateful to Y. Naka-gawa for her excellent secretarial assistance and to A. Sakai, M. Honda, H. Nakashima, K. Irie, and S. Kimura for technical help. We thank Dr T. Kitamura (Tokyo University) for pMY vector and Plat-E cells. This work was supported by grants from JSPS KAKENHI (15K14452) and MEXT KAKENHI (16H06527) to KN and MEXT KAKENHI (17H05647) to SK.
Publisher Copyright:
© 2017 Federation of European Biochemical Societies
PY - 2017/11
Y1 - 2017/11
N2 - Astrocytes, which support diverse neuronal functions, are generated from multipotent neural stem/precursor cells (NS/PCs) during brain development. Although many astrocyte-inducing factors have been identified and studied in vitro, the regions and/or cells that produce these factors in the developing brain remain elusive. Here, we show that meninges-produced factors induce astrocytic differentiation of NS/PCs. Consistent with the timing when astrocytic differentiation of NS/PCs increases, expression of astrocyte-inducing factors is upregulated. Meningeal secretion-mimicking combinatorial treatment of NS/PCs with bone morphogenetic protein 4, retinoic acid and leukemia inhibitory factor synergistically activate the promoter of a typical astrocytic marker, glial fibrillary acidic protein. Taken together, our data suggest that meninges play an important role in astrocytic differentiation of NS/PCs in the developing brain.
AB - Astrocytes, which support diverse neuronal functions, are generated from multipotent neural stem/precursor cells (NS/PCs) during brain development. Although many astrocyte-inducing factors have been identified and studied in vitro, the regions and/or cells that produce these factors in the developing brain remain elusive. Here, we show that meninges-produced factors induce astrocytic differentiation of NS/PCs. Consistent with the timing when astrocytic differentiation of NS/PCs increases, expression of astrocyte-inducing factors is upregulated. Meningeal secretion-mimicking combinatorial treatment of NS/PCs with bone morphogenetic protein 4, retinoic acid and leukemia inhibitory factor synergistically activate the promoter of a typical astrocytic marker, glial fibrillary acidic protein. Taken together, our data suggest that meninges play an important role in astrocytic differentiation of NS/PCs in the developing brain.
KW - astrocyte differentiation
KW - meninges
KW - neural stem/precursor cells
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U2 - 10.1002/1873-3468.12881
DO - 10.1002/1873-3468.12881
M3 - Article
C2 - 29029363
AN - SCOPUS:85033217630
SN - 0014-5793
VL - 591
SP - 3709
EP - 3720
JO - FEBS Letters
JF - FEBS Letters
IS - 22
ER -