Synthesis and secretion of human nerve growth factor by Saccharomyces cerevisiae

Kanaya Eiko, Higashizaki Takako, Ozawa Fumiko, Hirai Keiko, Nishizawa Masafumi, Tokiinaga Masao, Tsukui Hiroko, Hatanaka Hiroshi, Hishinuma Fumio

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The DNA coding for human nerve growth factor (hNGF) was chemically synthesized and introduced into Saccharomyces cerevisiae. Expression and secretion of hNGF was obtained by use of the yeast phosphoglycerate kinase-encoding gene promoter and the pre-pro sequence of the yeast α-mating factor. Immunoblotting with antiserum raised against a protein A-hNGF fusion protein, allowed the detection of an immunoreactive material secreted into the culture medium. A preparation from the culture medium, partially purified by ion-exchange column chromatography, stimulated neurite outgrowth from rat pheochromocytoma PC12h cells.

Original languageEnglish
Pages (from-to)65-74
Number of pages10
JournalGene
Volume83
Issue number1
DOIs
Publication statusPublished - 1989 Nov 15

Keywords

  • (Recombinant DNA
  • expression in yeast
  • neurite outgrowth)
  • pheochromocytoma cell
  • pre-pro sequence
  • processing of precursor protein

ASJC Scopus subject areas

  • Genetics

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  • Cite this

    Eiko, K., Takako, H., Fumiko, O., Keiko, H., Masafumi, N., Masao, T., Hiroko, T., Hiroshi, H., & Fumio, H. (1989). Synthesis and secretion of human nerve growth factor by Saccharomyces cerevisiae. Gene, 83(1), 65-74. https://doi.org/10.1016/0378-1119(89)90404-6