Synthesis of a Pentasaccharide Repeating Unit of Lipopolysaccharide Derived from Virulent E. coli O1 and Identification of a Glycotope Candidate of Avian Pathogenic E. coli O1

Nobuya Nishi, Katsunori Seki, Daisuke Takahashi, Kazunobu Toshima

Research output: Contribution to journalArticlepeer-review

Abstract

Avian pathogenic Escherichia coli (APEC) is a common bacterial pathogen infecting chickens, resulting in economic losses to the poultry industry worldwide. In particular, APEC O1, one of the most common serotypes of APEC, is considered problematic due to its zoonotic potential. Therefore, many attempts have been made to develop an effective vaccine against APEC O1. In fact, the lipopolysaccharide (LPS) O-antigen of APEC O1 has been shown to be a potent antigen for inducing specific protective immune responses. However, the detailed structure of the O-antigen of APEC O1 is not clear. The present study demonstrates the first synthesis of a pentasaccharide repeating unit of LPS derived from virulent E. coli O1 and its conjugate with BSA. ELISA tests using the semi-synthetic glycoconjugate and the APEC O1 immune chicken serum revealed that the pentasaccharide is a glycotope candidate of APEC O1, with great potential as an antigen for vaccine development.

Original languageEnglish
Pages (from-to)1789-1796
Number of pages8
JournalAngewandte Chemie - International Edition
Volume60
Issue number4
DOIs
Publication statusPublished - 2021 Jan 25

Keywords

  • avian pathogenic Escherichia coli
  • boron-mediated aglycon delivery
  • glycoconjugate
  • glycotope
  • β-rhamnoside

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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