Synthesis of fluorescent substrates for protein tyrosine phosphatase assays

Takumi Watanabe; Masaya Imoto; Hiroki Taniguchi; Kazuhiko Kinoshita; Kazuo Umezawa

doi:10.1016/S0960-894X(98)00208-X

Synthesis of fluorescent substrates for protein tyrosine phosphatase assays

Takumi Watanabe, Masaya Imoto, Hiroki Taniguchi, Kazuhiko Kinoshita, Kazuo Umezawa

Department of Biosciences and Informatics

Research output: Contribution to journal › Article › peer-review

Abstract

Two fluorescent substrates for protein tyrosine phosphatase (PTPase) reaction were prepared by conjugation of commercially available O- phosphotyrosine and dansyl chlorides. They were hydrolyzed by CD45 tyrosine phosphatase, and proved to be useful for PTPase assay.

Original language	English
Pages (from-to)	1301-1302
Number of pages	2
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	8
Issue number	11
DOIs	https://doi.org/10.1016/S0960-894X(98)00208-X
Publication status	Published - 1998 Jun 2

Keywords

Amino acids and derivatives
Enzyme inhibitors
Fluorescence

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

Access to Document

10.1016/S0960-894X(98)00208-X

Fingerprint Dive into the research topics of 'Synthesis of fluorescent substrates for protein tyrosine phosphatase assays'. Together they form a unique fingerprint.

Cite this

@article{4d0ae4ab04154e298c50778de124b8c1,

title = "Synthesis of fluorescent substrates for protein tyrosine phosphatase assays",

abstract = "Two fluorescent substrates for protein tyrosine phosphatase (PTPase) reaction were prepared by conjugation of commercially available O- phosphotyrosine and dansyl chlorides. They were hydrolyzed by CD45 tyrosine phosphatase, and proved to be useful for PTPase assay.",

keywords = "Amino acids and derivatives, Enzyme inhibitors, Fluorescence",

author = "Takumi Watanabe and Masaya Imoto and Hiroki Taniguchi and Kazuhiko Kinoshita and Kazuo Umezawa",

year = "1998",

month = jun,

day = "2",

doi = "10.1016/S0960-894X(98)00208-X",

language = "English",

volume = "8",

pages = "1301--1302",

journal = "Bioorganic and Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier Limited",

number = "11",

}

TY - JOUR

T1 - Synthesis of fluorescent substrates for protein tyrosine phosphatase assays

AU - Watanabe, Takumi

AU - Imoto, Masaya

AU - Taniguchi, Hiroki

AU - Kinoshita, Kazuhiko

AU - Umezawa, Kazuo

PY - 1998/6/2

Y1 - 1998/6/2

N2 - Two fluorescent substrates for protein tyrosine phosphatase (PTPase) reaction were prepared by conjugation of commercially available O- phosphotyrosine and dansyl chlorides. They were hydrolyzed by CD45 tyrosine phosphatase, and proved to be useful for PTPase assay.

AB - Two fluorescent substrates for protein tyrosine phosphatase (PTPase) reaction were prepared by conjugation of commercially available O- phosphotyrosine and dansyl chlorides. They were hydrolyzed by CD45 tyrosine phosphatase, and proved to be useful for PTPase assay.

KW - Amino acids and derivatives

KW - Enzyme inhibitors

KW - Fluorescence

UR - http://www.scopus.com/inward/record.url?scp=0032474522&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032474522&partnerID=8YFLogxK

U2 - 10.1016/S0960-894X(98)00208-X

DO - 10.1016/S0960-894X(98)00208-X

M3 - Article

C2 - 9871754

AN - SCOPUS:0032474522

VL - 8

SP - 1301

EP - 1302

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

SN - 0960-894X

IS - 11

ER -

Synthesis of fluorescent substrates for protein tyrosine phosphatase assays

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this