Synthesis of fluorescent substrates for protein tyrosine phosphatase assays

Takumi Watanabe, Masaya Imoto, Hiroki Taniguchi, Kazuhiko Kinoshita, Kazuo Umezawa

Research output: Contribution to journal › Article

Abstract

Two fluorescent substrates for protein tyrosine phosphatase (PTPase) reaction were prepared by conjugation of commercially available O- phosphotyrosine and dansyl chlorides. They were hydrolyzed by CD45 tyrosine phosphatase, and proved to be useful for PTPase assay.

Original languageEnglish
Pages (from-to)1301-1302
Number of pages2
JournalBioorganic and Medicinal Chemistry Letters
Volume8
Issue number11
DOIs
Publication statusPublished - 1998 Jun 2

Fingerprint

Protein Tyrosine Phosphatases
Assays
Phosphotyrosine
Substrates
Phosphoric Monoester Hydrolases
Tyrosine
dansyl chloride

Keywords

  • Amino acids and derivatives
  • Enzyme inhibitors
  • Fluorescence

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

Cite this

Synthesis of fluorescent substrates for protein tyrosine phosphatase assays. / Watanabe, Takumi; Imoto, Masaya; Taniguchi, Hiroki; Kinoshita, Kazuhiko; Umezawa, Kazuo.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 8, No. 11, 02.06.1998, p. 1301-1302.

Research output: Contribution to journal › Article

Watanabe, T, Imoto, M, Taniguchi, H, Kinoshita, K & Umezawa, K 1998, 'Synthesis of fluorescent substrates for protein tyrosine phosphatase assays', Bioorganic and Medicinal Chemistry Letters, vol. 8, no. 11, pp. 1301-1302. https://doi.org/10.1016/S0960-894X(98)00208-X
Watanabe T, Imoto M, Taniguchi H, Kinoshita K, Umezawa K. Synthesis of fluorescent substrates for protein tyrosine phosphatase assays. Bioorganic and Medicinal Chemistry Letters. 1998 Jun 2;8(11):1301-1302. https://doi.org/10.1016/S0960-894X(98)00208-X
Watanabe, Takumi ; Imoto, Masaya ; Taniguchi, Hiroki ; Kinoshita, Kazuhiko ; Umezawa, Kazuo. / Synthesis of fluorescent substrates for protein tyrosine phosphatase assays. In: Bioorganic and Medicinal Chemistry Letters. 1998 ; Vol. 8, No. 11. pp. 1301-1302.
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