TY - JOUR
T1 - Synthesis of fluorine substituted oligosaccharide analogues of monoglucosylated glycan chain, a proposed ligand of lectin-chaperone calreticulin and calnexin
AU - Ito, Yukishige
AU - Hagihara, Shinya
AU - Arai, Midori A.
AU - Matsuo, Ichiro
AU - Takatani, Maki
PY - 2004/11/1
Y1 - 2004/11/1
N2 - As a part of a exploring the N-glycan-mediated glycoprotein quality control in endoplasmic reticulum, 2-fluorinated derivative Glcα1 → 3Man(F) 1, Glcα1 → 3Man(F)α1 → 2Man2, and Glcα1 → 3Man(F)α1 → 2Manα1 → 2Man 3 were synthesized in a concise manner. These oligosaccharides were subjected to binding studies with calreticulin by using isothermal titration calorimetry. It was revealed that disaccharide 1 was a poor ligand, while tri- (2) and tetrasaccharide (3) had observable affinity.
AB - As a part of a exploring the N-glycan-mediated glycoprotein quality control in endoplasmic reticulum, 2-fluorinated derivative Glcα1 → 3Man(F) 1, Glcα1 → 3Man(F)α1 → 2Man2, and Glcα1 → 3Man(F)α1 → 2Manα1 → 2Man 3 were synthesized in a concise manner. These oligosaccharides were subjected to binding studies with calreticulin by using isothermal titration calorimetry. It was revealed that disaccharide 1 was a poor ligand, while tri- (2) and tetrasaccharide (3) had observable affinity.
KW - 2-fluorinated oligosaccharide
KW - calreticulin
KW - glycoprotein quality control
KW - synthesis
UR - http://www.scopus.com/inward/record.url?scp=6344241117&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=6344241117&partnerID=8YFLogxK
U2 - 10.1023/B:GLYC.0000045109.60425.2e
DO - 10.1023/B:GLYC.0000045109.60425.2e
M3 - Article
C2 - 15486458
AN - SCOPUS:6344241117
VL - 21
SP - 257
EP - 266
JO - Glycoconjugate Journal
JF - Glycoconjugate Journal
SN - 0282-0080
IS - 5
ER -