Synthesis of fluorine substituted oligosaccharide analogues of monoglucosylated glycan chain, a proposed ligand of lectin-chaperone calreticulin and calnexin

Yukishige Ito; Shinya Hagihara; Midori A. Arai; Ichiro Matsuo; Maki Takatani

doi:10.1023/B:GLYC.0000045109.60425.2e

Synthesis of fluorine substituted oligosaccharide analogues of monoglucosylated glycan chain, a proposed ligand of lectin-chaperone calreticulin and calnexin

Yukishige Ito, Shinya Hagihara, Midori A. Arai, Ichiro Matsuo, Maki Takatani

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

As a part of a exploring the N-glycan-mediated glycoprotein quality control in endoplasmic reticulum, 2-fluorinated derivative Glcα1 → 3Man(F) 1, Glcα1 → 3Man(F)α1 → 2Man2, and Glcα1 → 3Man(F)α1 → 2Manα1 → 2Man 3 were synthesized in a concise manner. These oligosaccharides were subjected to binding studies with calreticulin by using isothermal titration calorimetry. It was revealed that disaccharide 1 was a poor ligand, while tri- (2) and tetrasaccharide (3) had observable affinity.

Original language	English
Pages (from-to)	257-266
Number of pages	10
Journal	Glycoconjugate Journal
Volume	21
Issue number	5
DOIs	https://doi.org/10.1023/B:GLYC.0000045109.60425.2e
Publication status	Published - 2004 Nov 1
Externally published	Yes

Keywords

2-fluorinated oligosaccharide
calreticulin
glycoprotein quality control
synthesis

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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Synthesis of fluorine substituted oligosaccharide analogues of monoglucosylated glycan chain, a proposed ligand of lectin-chaperone calreticulin and calnexin. / Ito, Yukishige; Hagihara, Shinya; Arai, Midori A.; Matsuo, Ichiro; Takatani, Maki.

In: Glycoconjugate Journal, Vol. 21, No. 5, 01.11.2004, p. 257-266.

Research output: Contribution to journal › Article › peer-review

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