Synthesis of fluorine substituted oligosaccharide analogues of monoglucosylated glycan chain, a proposed ligand of lectin-chaperone calreticulin and calnexin

Yukishige Ito, Shinya Hagihara, Midori A. Arai, Ichiro Matsuo, Maki Takatani

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

As a part of a exploring the N-glycan-mediated glycoprotein quality control in endoplasmic reticulum, 2-fluorinated derivative Glcα1 → 3Man(F) 1, Glcα1 → 3Man(F)α1 → 2Man2, and Glcα1 → 3Man(F)α1 → 2Manα1 → 2Man 3 were synthesized in a concise manner. These oligosaccharides were subjected to binding studies with calreticulin by using isothermal titration calorimetry. It was revealed that disaccharide 1 was a poor ligand, while tri- (2) and tetrasaccharide (3) had observable affinity.

Original languageEnglish
Pages (from-to)257-266
Number of pages10
JournalGlycoconjugate Journal
Volume21
Issue number5
DOIs
Publication statusPublished - 2004 Nov 1
Externally publishedYes

Keywords

  • 2-fluorinated oligosaccharide
  • calreticulin
  • glycoprotein quality control
  • synthesis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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