Abstract
Nanosized porous hydroxyapatite (HA) granules are expected to be useful as DNA and drug carriers for cells. We attempted to synthesize nanosized HA granules in an agarose gel by electrophoresis of calcium and phosphate ions. Wells were prepared on an agarose gel, and a CaCl 2 solution was placed in the wells on the positive side and a Na 2HPO 4 solution was placed in the wells on the negative side. After a potential was applied, white precipitates appeared in the gel. The precipitates were calcium-deficient HA granules of approximately 300-600nm in diameter. These granules were composed of fiber-like particles and had a porous structure. The diameters of the granules were decreased by an increase in the gel concentration. Hence, we successfully synthesized nanosized porous HA granules to be used as DNA and drug carriers.
| Original language | English |
|---|---|
| Pages (from-to) | 236-239 |
| Number of pages | 4 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 97 |
| DOIs | |
| Publication status | Published - 2012 Sep 1 |
| Externally published | Yes |
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Keywords
- Carrier
- Electrophoresis
- Hydrogel
- Hydroxyapatite
- Nanosized granules
ASJC Scopus subject areas
- Biotechnology
- Colloid and Surface Chemistry
- Physical and Theoretical Chemistry
- Surfaces and Interfaces
Cite this
Synthesis of nanosized porous hydroxyapatite granules in hydrogel by electrophoresis. / Kamitakahara, Masanobu; Kimura, Kenshiro; Ioku, Koji.
In: Colloids and Surfaces B: Biointerfaces, Vol. 97, 01.09.2012, p. 236-239.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Synthesis of nanosized porous hydroxyapatite granules in hydrogel by electrophoresis
AU - Kamitakahara, Masanobu
AU - Kimura, Kenshiro
AU - Ioku, Koji
PY - 2012/9/1
Y1 - 2012/9/1
N2 - Nanosized porous hydroxyapatite (HA) granules are expected to be useful as DNA and drug carriers for cells. We attempted to synthesize nanosized HA granules in an agarose gel by electrophoresis of calcium and phosphate ions. Wells were prepared on an agarose gel, and a CaCl 2 solution was placed in the wells on the positive side and a Na 2HPO 4 solution was placed in the wells on the negative side. After a potential was applied, white precipitates appeared in the gel. The precipitates were calcium-deficient HA granules of approximately 300-600nm in diameter. These granules were composed of fiber-like particles and had a porous structure. The diameters of the granules were decreased by an increase in the gel concentration. Hence, we successfully synthesized nanosized porous HA granules to be used as DNA and drug carriers.
AB - Nanosized porous hydroxyapatite (HA) granules are expected to be useful as DNA and drug carriers for cells. We attempted to synthesize nanosized HA granules in an agarose gel by electrophoresis of calcium and phosphate ions. Wells were prepared on an agarose gel, and a CaCl 2 solution was placed in the wells on the positive side and a Na 2HPO 4 solution was placed in the wells on the negative side. After a potential was applied, white precipitates appeared in the gel. The precipitates were calcium-deficient HA granules of approximately 300-600nm in diameter. These granules were composed of fiber-like particles and had a porous structure. The diameters of the granules were decreased by an increase in the gel concentration. Hence, we successfully synthesized nanosized porous HA granules to be used as DNA and drug carriers.
KW - Carrier
KW - Electrophoresis
KW - Hydrogel
KW - Hydroxyapatite
KW - Nanosized granules
UR - http://www.scopus.com/inward/record.url?scp=84861082794&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84861082794&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2012.03.033
DO - 10.1016/j.colsurfb.2012.03.033
M3 - Article
C2 - 22609609
AN - SCOPUS:84861082794
VL - 97
SP - 236
EP - 239
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -