Synthesis of nanosized porous hydroxyapatite granules in hydrogel by electrophoresis

Masanobu Kamitakahara; Kenshiro Kimura; Koji Ioku

doi:10.1016/j.colsurfb.2012.03.033

Synthesis of nanosized porous hydroxyapatite granules in hydrogel by electrophoresis

Masanobu Kamitakahara, Kenshiro Kimura, Koji Ioku

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Nanosized porous hydroxyapatite (HA) granules are expected to be useful as DNA and drug carriers for cells. We attempted to synthesize nanosized HA granules in an agarose gel by electrophoresis of calcium and phosphate ions. Wells were prepared on an agarose gel, and a CaCl ₂ solution was placed in the wells on the positive side and a Na ₂HPO ₄ solution was placed in the wells on the negative side. After a potential was applied, white precipitates appeared in the gel. The precipitates were calcium-deficient HA granules of approximately 300-600nm in diameter. These granules were composed of fiber-like particles and had a porous structure. The diameters of the granules were decreased by an increase in the gel concentration. Hence, we successfully synthesized nanosized porous HA granules to be used as DNA and drug carriers.

Original language	English
Pages (from-to)	236-239
Number of pages	4
Journal	Colloids and Surfaces B: Biointerfaces
Volume	97
DOIs	https://doi.org/10.1016/j.colsurfb.2012.03.033
Publication status	Published - 2012 Sep 1
Externally published	Yes

Keywords

Carrier
Electrophoresis
Hydrogel
Hydroxyapatite
Nanosized granules

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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abstract = "Nanosized porous hydroxyapatite (HA) granules are expected to be useful as DNA and drug carriers for cells. We attempted to synthesize nanosized HA granules in an agarose gel by electrophoresis of calcium and phosphate ions. Wells were prepared on an agarose gel, and a CaCl 2 solution was placed in the wells on the positive side and a Na 2HPO 4 solution was placed in the wells on the negative side. After a potential was applied, white precipitates appeared in the gel. The precipitates were calcium-deficient HA granules of approximately 300-600nm in diameter. These granules were composed of fiber-like particles and had a porous structure. The diameters of the granules were decreased by an increase in the gel concentration. Hence, we successfully synthesized nanosized porous HA granules to be used as DNA and drug carriers.",

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AU - Kamitakahara, Masanobu

AU - Kimura, Kenshiro

AU - Ioku, Koji

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Y1 - 2012/9/1

N2 - Nanosized porous hydroxyapatite (HA) granules are expected to be useful as DNA and drug carriers for cells. We attempted to synthesize nanosized HA granules in an agarose gel by electrophoresis of calcium and phosphate ions. Wells were prepared on an agarose gel, and a CaCl 2 solution was placed in the wells on the positive side and a Na 2HPO 4 solution was placed in the wells on the negative side. After a potential was applied, white precipitates appeared in the gel. The precipitates were calcium-deficient HA granules of approximately 300-600nm in diameter. These granules were composed of fiber-like particles and had a porous structure. The diameters of the granules were decreased by an increase in the gel concentration. Hence, we successfully synthesized nanosized porous HA granules to be used as DNA and drug carriers.

AB - Nanosized porous hydroxyapatite (HA) granules are expected to be useful as DNA and drug carriers for cells. We attempted to synthesize nanosized HA granules in an agarose gel by electrophoresis of calcium and phosphate ions. Wells were prepared on an agarose gel, and a CaCl 2 solution was placed in the wells on the positive side and a Na 2HPO 4 solution was placed in the wells on the negative side. After a potential was applied, white precipitates appeared in the gel. The precipitates were calcium-deficient HA granules of approximately 300-600nm in diameter. These granules were composed of fiber-like particles and had a porous structure. The diameters of the granules were decreased by an increase in the gel concentration. Hence, we successfully synthesized nanosized porous HA granules to be used as DNA and drug carriers.

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