Synthesis of nanosized porous hydroxyapatite granules in hydrogel by electrophoresis

Masanobu Kamitakahara; Kenshiro Kimura; Koji Ioku

doi:10.1016/j.colsurfb.2012.03.033

Synthesis of nanosized porous hydroxyapatite granules in hydrogel by electrophoresis

Masanobu Kamitakahara, Kenshiro Kimura, Koji Ioku

Research output: Contribution to journal › Article

12 Citations (Scopus)

Abstract

Nanosized porous hydroxyapatite (HA) granules are expected to be useful as DNA and drug carriers for cells. We attempted to synthesize nanosized HA granules in an agarose gel by electrophoresis of calcium and phosphate ions. Wells were prepared on an agarose gel, and a CaCl ₂ solution was placed in the wells on the positive side and a Na ₂HPO ₄ solution was placed in the wells on the negative side. After a potential was applied, white precipitates appeared in the gel. The precipitates were calcium-deficient HA granules of approximately 300-600nm in diameter. These granules were composed of fiber-like particles and had a porous structure. The diameters of the granules were decreased by an increase in the gel concentration. Hence, we successfully synthesized nanosized porous HA granules to be used as DNA and drug carriers.

Original language	English
Pages (from-to)	236-239
Number of pages	4
Journal	Colloids and Surfaces B: Biointerfaces
Volume	97
DOIs	https://doi.org/10.1016/j.colsurfb.2012.03.033
Publication status	Published - 2012 Sep 1
Externally published	Yes

Keywords

Carrier
Electrophoresis
Hydrogel
Hydroxyapatite
Nanosized granules

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

Access to Document

10.1016/j.colsurfb.2012.03.033

Fingerprint Dive into the research topics of 'Synthesis of nanosized porous hydroxyapatite granules in hydrogel by electrophoresis'. Together they form a unique fingerprint.

Cite this

Kamitakahara, M., Kimura, K., & Ioku, K. (2012). Synthesis of nanosized porous hydroxyapatite granules in hydrogel by electrophoresis. Colloids and Surfaces B: Biointerfaces, 97, 236-239. https://doi.org/10.1016/j.colsurfb.2012.03.033

@article{44f1a0be188046b6a78223c3c74fa06e,

title = "Synthesis of nanosized porous hydroxyapatite granules in hydrogel by electrophoresis",

abstract = "Nanosized porous hydroxyapatite (HA) granules are expected to be useful as DNA and drug carriers for cells. We attempted to synthesize nanosized HA granules in an agarose gel by electrophoresis of calcium and phosphate ions. Wells were prepared on an agarose gel, and a CaCl 2 solution was placed in the wells on the positive side and a Na 2HPO 4 solution was placed in the wells on the negative side. After a potential was applied, white precipitates appeared in the gel. The precipitates were calcium-deficient HA granules of approximately 300-600nm in diameter. These granules were composed of fiber-like particles and had a porous structure. The diameters of the granules were decreased by an increase in the gel concentration. Hence, we successfully synthesized nanosized porous HA granules to be used as DNA and drug carriers.",

keywords = "Carrier, Electrophoresis, Hydrogel, Hydroxyapatite, Nanosized granules",

author = "Masanobu Kamitakahara and Kenshiro Kimura and Koji Ioku",

year = "2012",

month = sep,

day = "1",

doi = "10.1016/j.colsurfb.2012.03.033",

language = "English",

volume = "97",

pages = "236--239",

journal = "Colloids and Surfaces B: Biointerfaces",

issn = "0927-7765",

publisher = "Elsevier",

}

TY - JOUR

T1 - Synthesis of nanosized porous hydroxyapatite granules in hydrogel by electrophoresis

AU - Kamitakahara, Masanobu

AU - Kimura, Kenshiro

AU - Ioku, Koji

PY - 2012/9/1

Y1 - 2012/9/1

N2 - Nanosized porous hydroxyapatite (HA) granules are expected to be useful as DNA and drug carriers for cells. We attempted to synthesize nanosized HA granules in an agarose gel by electrophoresis of calcium and phosphate ions. Wells were prepared on an agarose gel, and a CaCl 2 solution was placed in the wells on the positive side and a Na 2HPO 4 solution was placed in the wells on the negative side. After a potential was applied, white precipitates appeared in the gel. The precipitates were calcium-deficient HA granules of approximately 300-600nm in diameter. These granules were composed of fiber-like particles and had a porous structure. The diameters of the granules were decreased by an increase in the gel concentration. Hence, we successfully synthesized nanosized porous HA granules to be used as DNA and drug carriers.

AB - Nanosized porous hydroxyapatite (HA) granules are expected to be useful as DNA and drug carriers for cells. We attempted to synthesize nanosized HA granules in an agarose gel by electrophoresis of calcium and phosphate ions. Wells were prepared on an agarose gel, and a CaCl 2 solution was placed in the wells on the positive side and a Na 2HPO 4 solution was placed in the wells on the negative side. After a potential was applied, white precipitates appeared in the gel. The precipitates were calcium-deficient HA granules of approximately 300-600nm in diameter. These granules were composed of fiber-like particles and had a porous structure. The diameters of the granules were decreased by an increase in the gel concentration. Hence, we successfully synthesized nanosized porous HA granules to be used as DNA and drug carriers.

KW - Carrier

KW - Electrophoresis

KW - Hydrogel

KW - Hydroxyapatite

KW - Nanosized granules

UR - http://www.scopus.com/inward/record.url?scp=84861082794&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861082794&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2012.03.033

DO - 10.1016/j.colsurfb.2012.03.033

M3 - Article

C2 - 22609609

AN - SCOPUS:84861082794

VL - 97

SP - 236

EP - 239

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

ER -