Synthetic bioluminescent coelenterazine derivatives

Ryo Nishihara, Daniel Citterio, Koji Suzuki

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The development of coelenterazine (CTZ) derivatives resulting in superior optical characteristics is an efficient method to extend the range of its possible applications. Here, we describe the synthesis of three C-6 substituted CTZ derivatives retaining the recognition by Renilla luciferase (RLuc) and its derivatives. The novel derivatives are useful as bright blue-shifted CTZ derivatives, which can be used as an alternative to hitherto reported compound DeepBlueC™.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages19-31
Number of pages13
Volume1461
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1461
ISSN (Print)10643745

Fingerprint

Renilla Luciferases
coelenterazine

Keywords

  • Bioluminescence
  • Chemiluminescence
  • Coelenterazine (CTZ)
  • DeepBlueC
  • Luciferase
  • Luciferin
  • Renilla luciferase (RLuc)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Nishihara, R., Citterio, D., & Suzuki, K. (2016). Synthetic bioluminescent coelenterazine derivatives. In Methods in Molecular Biology (Vol. 1461, pp. 19-31). (Methods in Molecular Biology; Vol. 1461). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3813-1_2

Synthetic bioluminescent coelenterazine derivatives. / Nishihara, Ryo; Citterio, Daniel; Suzuki, Koji.

Methods in Molecular Biology. Vol. 1461 Humana Press Inc., 2016. p. 19-31 (Methods in Molecular Biology; Vol. 1461).

Research output: Chapter in Book/Report/Conference proceedingChapter

Nishihara, R, Citterio, D & Suzuki, K 2016, Synthetic bioluminescent coelenterazine derivatives. in Methods in Molecular Biology. vol. 1461, Methods in Molecular Biology, vol. 1461, Humana Press Inc., pp. 19-31. https://doi.org/10.1007/978-1-4939-3813-1_2
Nishihara R, Citterio D, Suzuki K. Synthetic bioluminescent coelenterazine derivatives. In Methods in Molecular Biology. Vol. 1461. Humana Press Inc. 2016. p. 19-31. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3813-1_2
Nishihara, Ryo ; Citterio, Daniel ; Suzuki, Koji. / Synthetic bioluminescent coelenterazine derivatives. Methods in Molecular Biology. Vol. 1461 Humana Press Inc., 2016. pp. 19-31 (Methods in Molecular Biology).
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