Synthetic Biomarker Design by Using Analyte-Responsive Acetaminophen

Tatsuya Nishihara, Joe Inoue, Sho Tabata, Shinnosuke Murakami, Takamasa Ishikawa, Natsumi Saito, Shinji Fukuda, Masaru Tomita, Tomoyoshi Soga

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The use of synthetic biomarkers is an emerging technique to improve disease diagnosis. Here, we report a novel design strategy that uses analyte-responsive acetaminophen (APAP) to expand the catalogue of analytes available for synthetic biomarker development. As proof-of-concept, we designed hydrogen peroxide (H2O2)-responsive APAP (HR-APAP) and succeeded in H2O2 detection with cellular and animal experiments. In fact, for blood samples following HR-APAP injection, we demonstrated that the plasma concentration ratio [APAP+APAP conjugates]/[HR-APAP] accurately reflects in vivo differences in H2O2 levels. We anticipate that our practical methodology will be broadly useful for the preparation of various synthetic biomarkers.

Original languageEnglish
Pages (from-to)910-913
Number of pages4
JournalChemBioChem
Volume18
Issue number10
DOIs
Publication statusPublished - 2017 May 18

Keywords

  • acetaminophen
  • hydrogen peroxide
  • liquid chromatography
  • mass spectrometry
  • synthetic biomarker

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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  • Cite this

    Nishihara, T., Inoue, J., Tabata, S., Murakami, S., Ishikawa, T., Saito, N., Fukuda, S., Tomita, M., & Soga, T. (2017). Synthetic Biomarker Design by Using Analyte-Responsive Acetaminophen. ChemBioChem, 18(10), 910-913. https://doi.org/10.1002/cbic.201700023