@article{cee0d50707f24805a2fe2fb59779ecf4,
title = "Systematics of the widely distributed Japanese clouded salamander, hynobius nebulosus (Amphibia: Caudata: Hynobiidae), and its closest relatives",
abstract = "The abundant clouded salamander from western Japan, Hynobius nebulosus, has long been considered a single widespread species, although some authors have suggested the inclusion of several cryptic species. This led to a molecular and morphological analysis of populations from all parts of the known range. Phylogenetic relationships were inferred from complete sequences of the mitochondrial cytochrome b gene, and nuclear genome differentiations were estimated by multiplexed inter simple sequence repeat genotyping by sequencing (MIG-seq). The results suggest that H. nebulosus hitherto recognized consists of at least nine species. We applied existing names to two of them, H. nebulosus (Temminck et Schlegel) and H. vandenburghi Dunn, and described seven others as new species.",
keywords = "Cryptic species, Japan, New species, Phylogeny, Taxonomy",
author = "Masafumi Matsui and Hiroshi Okawa and Kanto Nishikawa and Gen Aoki and Koshiro Eto and Natsuhiko Yoshikawa and Shingo Tanabe and Yasuchika Misawa and Atsushi Tominaga",
note = "Funding Information: We thank K. Shimizu for providing valuable reprints of old literature, S. Akiyama, K. Araya, T. Hayashi, E. Honda, S. Ichihara, I. Ide, S. Ikeda, T. Iwata, T. Izumi, K. Kasugai, K. Kikusui, S. Kuzumi, T. Matsuo, I. Moriwaki, Y. Nakano, K. Niwa, H. Noda, S. Okada, T. Okayama, I. Okochi, T. Okuno, S. Saito, K. Sakakibara, S. Sato, T. Seto, N. Shinohara, M. Suda, T. Sugahara, M. Takagi, K. Tatewaki, T. Tochimoto, Y. Tomida, T. Watabe, and Y. Yuasa for providing locality information and helping to collect specimens. We would also like to thank an anonymous reviewer and K. Adler for their help in greatly improving an earlier version of the manuscript and reviewing English text. This work was supported by Grants-in-Aid to M. Matsui from the Ministry of Education, Science and Culture, Japan, and a grant from the Ministry of the Environment of Japan (nos. 01304001, 11640697, 63540599). This work was also partly supported by grants from the Showa Seitoku Memorial Foundation (2006) and the Fujiwara Natural History Foundation (2007) to K. Nishikawa, and the National Museum of Nature and Science, Tokyo (20172151, 20186211) to N. Yoshikawa. Funding Information: We thank K. Shimizu for providing valuable reprints of old literature, S. Akiyama, K. Araya, T. Hayashi, E. Honda, S. Ichihara, I. Ide, S. Ikeda, T. Iwata, T. Izumi, K. Kasugai, K. Kikusui, S. Kuzumi, T. Matsuo, I. Mori-waki, Y. Nakano, K. Niwa, H. Noda, S. Okada, T. Okayama, I. Okochi, T. Okuno, S. Saito, K. Sakakibara, S. Sato, T. Seto, N. Shinohara, M. Suda, T. Sugahara, M. Takagi, K. Tatewaki, T. Tochimoto, Y. Tomida, T. Watabe, and Y. Yuasa for providing locality information and helping to collect specimens. We would also like to thank an anonymous reviewer and K. Adler for their help in greatly improving an earlier version of the manuscript and reviewing English text. This work was supported by Grants-in-Aid to M. Matsui from the Ministry of Education, Science and Culture, Japan, and a grant from the Ministry of the Environment of Japan (nos. 01304001, 11640697, 63540599). This work was also partly supported by grants from the Showa Seitoku Memorial Foundation (2006) and the Fujiwara Natural History Foundation (2007) to K. Nishikawa, and the National Museum of Nature and Science, Tokyo (20172151, 20186211) to N. Yoshikawa. Publisher Copyright: {\textcopyright} 2019 by The Herpetological Society of Japan.",
year = "2019",
month = feb,
doi = "10.5358/hsj.38.32",
language = "English",
volume = "38",
pages = "32--90",
journal = "Current Herpetology",
issn = "1345-5834",
publisher = "Herpetological Society of Japan",
number = "1",
}