Systemic, but not intestinal, IL-7 is essential for the persistence of chronic colitis

Takayuki Tomita, Takanori Kanai, Yasuhiro Nemoto, Teruji Totsuka, Ryuichi Okamoto, Kiichiro Tsuchiya, Naoya Sakamoto, Mamoru Watanabe

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We previously demonstrated that IL-7 is produced by intestinal goblet cells and is essential for the persistence of colitis. It is well known, however, that goblet cells are decreased or depleted in the chronically inflamed mucosa of animal colitis models or human inflammatory bowel diseases. Thus, in this study, we assess whether intestinal IL-7 is surely required for the persistence of colitis using a RAG-1/2-/- colitis model induced by the adoptive transfer of CD4+CD45RBhigh T cells in combination with parabiosis system. Surprisingly, both IL-7-/- x RAG-1-/- and IL-7+/+ x RAG-1-/- host mice developed colitis 4 wk after parabiosis to a similar extent of colitic IL-7+/+ x RAG-1 -/- donor mice that were previously transferred with CD4 +CD45RBhigh T cells. Of note, although the number of CD4+ T cells recovered from the spleen or the bone marrow of IL-7-/- x RAG-1-/- host mice was significantly decreased compared with that of IL-7+/+ x RAG-1-/- host mice, an equivalent number of CD4+ T cells was recovered from the lamina propria of both mice, indicating that the expansion of CD4+ T cells in the spleen or in the bone marrow is dependent on IL-7, but not in the lamina propria. Development of colitis was never observed in parabionts between IL-7+/+ x RAG-1-/- host and noncolitic IL-7-/- x RAG-1-/- donor mice that were transferred with CD4 +CD45RBhigh T cells. Collectively, systemic, but not intestinal, IL-7 is essential for the persistence of colitis, suggesting that therapeutic approaches targeting the systemic IL-7/IL-7R signaling pathway may be feasible in the treatment of inflammatory bowel diseases.

Original languageEnglish
Pages (from-to)383-390
Number of pages8
JournalJournal of Immunology
Volume180
Issue number1
Publication statusPublished - 2008 Jan 1
Externally publishedYes

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Interleukin-7
Colitis
T-Lymphocytes
Parabiosis
Mucous Membrane
Goblet Cells
Inflammatory Bowel Diseases
Spleen
Bone Marrow
Adoptive Transfer
Animal Models

ASJC Scopus subject areas

  • Immunology

Cite this

Tomita, T., Kanai, T., Nemoto, Y., Totsuka, T., Okamoto, R., Tsuchiya, K., ... Watanabe, M. (2008). Systemic, but not intestinal, IL-7 is essential for the persistence of chronic colitis. Journal of Immunology, 180(1), 383-390.

Systemic, but not intestinal, IL-7 is essential for the persistence of chronic colitis. / Tomita, Takayuki; Kanai, Takanori; Nemoto, Yasuhiro; Totsuka, Teruji; Okamoto, Ryuichi; Tsuchiya, Kiichiro; Sakamoto, Naoya; Watanabe, Mamoru.

In: Journal of Immunology, Vol. 180, No. 1, 01.01.2008, p. 383-390.

Research output: Contribution to journalArticle

Tomita, T, Kanai, T, Nemoto, Y, Totsuka, T, Okamoto, R, Tsuchiya, K, Sakamoto, N & Watanabe, M 2008, 'Systemic, but not intestinal, IL-7 is essential for the persistence of chronic colitis', Journal of Immunology, vol. 180, no. 1, pp. 383-390.
Tomita T, Kanai T, Nemoto Y, Totsuka T, Okamoto R, Tsuchiya K et al. Systemic, but not intestinal, IL-7 is essential for the persistence of chronic colitis. Journal of Immunology. 2008 Jan 1;180(1):383-390.
Tomita, Takayuki ; Kanai, Takanori ; Nemoto, Yasuhiro ; Totsuka, Teruji ; Okamoto, Ryuichi ; Tsuchiya, Kiichiro ; Sakamoto, Naoya ; Watanabe, Mamoru. / Systemic, but not intestinal, IL-7 is essential for the persistence of chronic colitis. In: Journal of Immunology. 2008 ; Vol. 180, No. 1. pp. 383-390.
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