T-helper 17 and interleukin-17-producing lymphoid tissue inducer-like cells make different contributions to colitis in mice

Yuichi Ono, Takanori Kanai, Tomohisa Sujino, Yasuhiro Nemoto, Yasumasa Kanai, Yohei Mikami, Atsushi Hayashi, Atsuhiro Matsumoto, Hiromasa Takaishi, Haruhiko Ogata, Katsuyoshi Matsuoka, Tadakazu Hisamatsu, Mamoru Watanabe, Toshifumi Hibi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background & Aims: T helper (Th) 17 cells that express the retinoid-related orphan receptor (ROR) γt contribute to the development of colitis in mice, yet are found in normal and inflamed intestine. We investigated their development and functions in intestines of mice. Methods: We analyzed intestinal Th17 cells in healthy and inflamed intestinal tissues of mice. We analyzed expression of lymphotoxin (LT)α by Th17 cells and lymphoid tissue inducer-like cells. Results: LTα-/- and RORγt-/- mice had significantly lower percentages of naturally occurring Th17 cells in the small intestine than wild-type mice. Numbers of CD3-CD4+/-interleukin-7Rα+c-kit +CCR6+NKp46- lymphoid tissue inducer-like cells that produce interleukin-17A were increased in LTα-/- and LTα-/-×recombination activating gene (RAG)-2 -/- mice, compared with wild-type mice, but were absent from RORγt-/- mice. Parabiosis of wild-type and LTα -/- mice and bone marrow transplant experiments revealed that LTα-dependent gut-associated lymphoid tissue structures are required for generation of naturally occurring Th17 cells. However, when wild-type or LTα-/- CD4+CD45RBhigh T cells were transferred to RAG-2-/- or LTα-/-×RAG-2 -/- mice, all groups, irrespective of the presence or absence of LTα on the donor or recipient cells, developed colitis and generated Th1, Th17, and Th17/Th1 cells. RAG-2-/- mice that received a second round of transplantation, with colitogenic but not naturally occurring Th17 cells, developed intestinal inflammation. The presence of naturally occurring Th17 cells in the colons of mice inhibited development of colitis after transfer of CD4+CD45RBhigh T cells and increased the numbers of Foxp3+ cells derived from CD4+CD45RBhigh T cells. Conclusions: Gut-associated lymphoid tissue structures are required to generate naturally occurring Th17 cells that have regulatory activities in normal intestines of mice, but not for colitogenic Th17 and Th17/Th1 cells during inflammation.

Original languageEnglish
Pages (from-to)1288-1297
Number of pages10
JournalGastroenterology
Volume143
Issue number5
DOIs
Publication statusPublished - 2012 Nov

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Interleukin-17
Lymphoid Tissue
Colitis
Helper-Inducer T-Lymphocytes
Th17 Cells
Lymphotoxin-alpha
Genetic Recombination
Intestines
Th1 Cells
T-Lymphocytes
Genes
Parabiosis
Inflammation
Interleukins
Retinoids
Small Intestine
Colon
Cell Count
Transplantation

Keywords

  • Immune Regulation
  • Inflammatory Bowel Disease
  • Mouse Model
  • T-Cell Development

ASJC Scopus subject areas

  • Gastroenterology

Cite this

T-helper 17 and interleukin-17-producing lymphoid tissue inducer-like cells make different contributions to colitis in mice. / Ono, Yuichi; Kanai, Takanori; Sujino, Tomohisa; Nemoto, Yasuhiro; Kanai, Yasumasa; Mikami, Yohei; Hayashi, Atsushi; Matsumoto, Atsuhiro; Takaishi, Hiromasa; Ogata, Haruhiko; Matsuoka, Katsuyoshi; Hisamatsu, Tadakazu; Watanabe, Mamoru; Hibi, Toshifumi.

In: Gastroenterology, Vol. 143, No. 5, 11.2012, p. 1288-1297.

Research output: Contribution to journalArticle

Ono, Y, Kanai, T, Sujino, T, Nemoto, Y, Kanai, Y, Mikami, Y, Hayashi, A, Matsumoto, A, Takaishi, H, Ogata, H, Matsuoka, K, Hisamatsu, T, Watanabe, M & Hibi, T 2012, 'T-helper 17 and interleukin-17-producing lymphoid tissue inducer-like cells make different contributions to colitis in mice', Gastroenterology, vol. 143, no. 5, pp. 1288-1297. https://doi.org/10.1053/j.gastro.2012.07.108
Ono, Yuichi ; Kanai, Takanori ; Sujino, Tomohisa ; Nemoto, Yasuhiro ; Kanai, Yasumasa ; Mikami, Yohei ; Hayashi, Atsushi ; Matsumoto, Atsuhiro ; Takaishi, Hiromasa ; Ogata, Haruhiko ; Matsuoka, Katsuyoshi ; Hisamatsu, Tadakazu ; Watanabe, Mamoru ; Hibi, Toshifumi. / T-helper 17 and interleukin-17-producing lymphoid tissue inducer-like cells make different contributions to colitis in mice. In: Gastroenterology. 2012 ; Vol. 143, No. 5. pp. 1288-1297.
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AU - Ono, Yuichi

AU - Kanai, Takanori

AU - Sujino, Tomohisa

AU - Nemoto, Yasuhiro

AU - Kanai, Yasumasa

AU - Mikami, Yohei

AU - Hayashi, Atsushi

AU - Matsumoto, Atsuhiro

AU - Takaishi, Hiromasa

AU - Ogata, Haruhiko

AU - Matsuoka, Katsuyoshi

AU - Hisamatsu, Tadakazu

AU - Watanabe, Mamoru

AU - Hibi, Toshifumi

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N2 - Background & Aims: T helper (Th) 17 cells that express the retinoid-related orphan receptor (ROR) γt contribute to the development of colitis in mice, yet are found in normal and inflamed intestine. We investigated their development and functions in intestines of mice. Methods: We analyzed intestinal Th17 cells in healthy and inflamed intestinal tissues of mice. We analyzed expression of lymphotoxin (LT)α by Th17 cells and lymphoid tissue inducer-like cells. Results: LTα-/- and RORγt-/- mice had significantly lower percentages of naturally occurring Th17 cells in the small intestine than wild-type mice. Numbers of CD3-CD4+/-interleukin-7Rα+c-kit +CCR6+NKp46- lymphoid tissue inducer-like cells that produce interleukin-17A were increased in LTα-/- and LTα-/-×recombination activating gene (RAG)-2 -/- mice, compared with wild-type mice, but were absent from RORγt-/- mice. Parabiosis of wild-type and LTα -/- mice and bone marrow transplant experiments revealed that LTα-dependent gut-associated lymphoid tissue structures are required for generation of naturally occurring Th17 cells. However, when wild-type or LTα-/- CD4+CD45RBhigh T cells were transferred to RAG-2-/- or LTα-/-×RAG-2 -/- mice, all groups, irrespective of the presence or absence of LTα on the donor or recipient cells, developed colitis and generated Th1, Th17, and Th17/Th1 cells. RAG-2-/- mice that received a second round of transplantation, with colitogenic but not naturally occurring Th17 cells, developed intestinal inflammation. The presence of naturally occurring Th17 cells in the colons of mice inhibited development of colitis after transfer of CD4+CD45RBhigh T cells and increased the numbers of Foxp3+ cells derived from CD4+CD45RBhigh T cells. Conclusions: Gut-associated lymphoid tissue structures are required to generate naturally occurring Th17 cells that have regulatory activities in normal intestines of mice, but not for colitogenic Th17 and Th17/Th1 cells during inflammation.

AB - Background & Aims: T helper (Th) 17 cells that express the retinoid-related orphan receptor (ROR) γt contribute to the development of colitis in mice, yet are found in normal and inflamed intestine. We investigated their development and functions in intestines of mice. Methods: We analyzed intestinal Th17 cells in healthy and inflamed intestinal tissues of mice. We analyzed expression of lymphotoxin (LT)α by Th17 cells and lymphoid tissue inducer-like cells. Results: LTα-/- and RORγt-/- mice had significantly lower percentages of naturally occurring Th17 cells in the small intestine than wild-type mice. Numbers of CD3-CD4+/-interleukin-7Rα+c-kit +CCR6+NKp46- lymphoid tissue inducer-like cells that produce interleukin-17A were increased in LTα-/- and LTα-/-×recombination activating gene (RAG)-2 -/- mice, compared with wild-type mice, but were absent from RORγt-/- mice. Parabiosis of wild-type and LTα -/- mice and bone marrow transplant experiments revealed that LTα-dependent gut-associated lymphoid tissue structures are required for generation of naturally occurring Th17 cells. However, when wild-type or LTα-/- CD4+CD45RBhigh T cells were transferred to RAG-2-/- or LTα-/-×RAG-2 -/- mice, all groups, irrespective of the presence or absence of LTα on the donor or recipient cells, developed colitis and generated Th1, Th17, and Th17/Th1 cells. RAG-2-/- mice that received a second round of transplantation, with colitogenic but not naturally occurring Th17 cells, developed intestinal inflammation. The presence of naturally occurring Th17 cells in the colons of mice inhibited development of colitis after transfer of CD4+CD45RBhigh T cells and increased the numbers of Foxp3+ cells derived from CD4+CD45RBhigh T cells. Conclusions: Gut-associated lymphoid tissue structures are required to generate naturally occurring Th17 cells that have regulatory activities in normal intestines of mice, but not for colitogenic Th17 and Th17/Th1 cells during inflammation.

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