Abstract
The contribution to atherosclerosis of two CX3CR1 single nucleotide polymorphisms, V249I and T280M has been recently reported. The atherosclerosis of intracranial vessels is thought to be the major pathological mechanism of ischemic stroke. In this study, we investigated the risk of ischemic stroke associated with fractalkine receptor CX3CR1 polymorphisms. We investigated the T280M and V249I mutations in the CX3CR1 gene in 235 Japanese patients with ischemic cerebrovascular disease (CVD) and 306 age- and sex-matched healthy controls. Polymerase chain reaction and restriction fragment length polymorphism were used for genotyping. There was no significant difference in both polymorphisms between patients with ischemic CVD and controls (VV versus II + VI, p = 0.83; TT versus MM + TM, p = 0.66). The I and M allele frequencies were not significantly different between CVD patients and controls: odds ratio (OR) = 0.89 (95% confidence interval (CI) = 0.50-1.60, p = 0.70) and OR = 1.19 (95% CI = 0.71-2.00, p = 0.51), respectively. We found eight of nine possible combined genotypes, including a new haplotype V249-M280, in Japanese. Our results show that these CX3CR1 gene polymorphisms are not associated with an increased risk for ischemic CVD in the Japanese population.
| Original language | English |
|---|---|
| Pages (from-to) | 132-135 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 374 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2005 Feb 10 |
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Keywords
- Atherosclerosis
- CX3CR1
- Fractalkine
- Ischemic stroke
- Polymorphism
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
T280M and V249I polymorphisms of fractalkine receptor CX3CR1 and ischemic cerebrovascular disease. / Hattori, Hidenori; Ito, Daisuke; Tanahashi, Norio; Murata, Mitsuru; Saito, Ikuo; Watanabe, Kiyoaki; Suzuki, Norihiro.
In: Neuroscience Letters, Vol. 374, No. 2, 10.02.2005, p. 132-135.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - T280M and V249I polymorphisms of fractalkine receptor CX3CR1 and ischemic cerebrovascular disease
AU - Hattori, Hidenori
AU - Ito, Daisuke
AU - Tanahashi, Norio
AU - Murata, Mitsuru
AU - Saito, Ikuo
AU - Watanabe, Kiyoaki
AU - Suzuki, Norihiro
PY - 2005/2/10
Y1 - 2005/2/10
N2 - The contribution to atherosclerosis of two CX3CR1 single nucleotide polymorphisms, V249I and T280M has been recently reported. The atherosclerosis of intracranial vessels is thought to be the major pathological mechanism of ischemic stroke. In this study, we investigated the risk of ischemic stroke associated with fractalkine receptor CX3CR1 polymorphisms. We investigated the T280M and V249I mutations in the CX3CR1 gene in 235 Japanese patients with ischemic cerebrovascular disease (CVD) and 306 age- and sex-matched healthy controls. Polymerase chain reaction and restriction fragment length polymorphism were used for genotyping. There was no significant difference in both polymorphisms between patients with ischemic CVD and controls (VV versus II + VI, p = 0.83; TT versus MM + TM, p = 0.66). The I and M allele frequencies were not significantly different between CVD patients and controls: odds ratio (OR) = 0.89 (95% confidence interval (CI) = 0.50-1.60, p = 0.70) and OR = 1.19 (95% CI = 0.71-2.00, p = 0.51), respectively. We found eight of nine possible combined genotypes, including a new haplotype V249-M280, in Japanese. Our results show that these CX3CR1 gene polymorphisms are not associated with an increased risk for ischemic CVD in the Japanese population.
AB - The contribution to atherosclerosis of two CX3CR1 single nucleotide polymorphisms, V249I and T280M has been recently reported. The atherosclerosis of intracranial vessels is thought to be the major pathological mechanism of ischemic stroke. In this study, we investigated the risk of ischemic stroke associated with fractalkine receptor CX3CR1 polymorphisms. We investigated the T280M and V249I mutations in the CX3CR1 gene in 235 Japanese patients with ischemic cerebrovascular disease (CVD) and 306 age- and sex-matched healthy controls. Polymerase chain reaction and restriction fragment length polymorphism were used for genotyping. There was no significant difference in both polymorphisms between patients with ischemic CVD and controls (VV versus II + VI, p = 0.83; TT versus MM + TM, p = 0.66). The I and M allele frequencies were not significantly different between CVD patients and controls: odds ratio (OR) = 0.89 (95% confidence interval (CI) = 0.50-1.60, p = 0.70) and OR = 1.19 (95% CI = 0.71-2.00, p = 0.51), respectively. We found eight of nine possible combined genotypes, including a new haplotype V249-M280, in Japanese. Our results show that these CX3CR1 gene polymorphisms are not associated with an increased risk for ischemic CVD in the Japanese population.
KW - Atherosclerosis
KW - CX3CR1
KW - Fractalkine
KW - Ischemic stroke
KW - Polymorphism
UR - http://www.scopus.com/inward/record.url?scp=11844282848&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=11844282848&partnerID=8YFLogxK
U2 - 10.1016/j.neulet.2004.10.042
DO - 10.1016/j.neulet.2004.10.042
M3 - Article
VL - 374
SP - 132
EP - 135
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 2
ER -