Tagging of avidin immobilized beads with biotinylated YAG:Ce3+ nanocrystal phosphor

Ryo Asakura; Tetsuhiko Isobe; Kiyoshi Kurokawa; Hideki Aizawa; Michio Ohkubo

doi:10.1007/s00216-006-0814-6

Tagging of avidin immobilized beads with biotinylated YAG:Ce³⁺ nanocrystal phosphor

Ryo Asakura, Tetsuhiko Isobe, Kiyoshi Kurokawa, Hideki Aizawa, Michio Ohkubo

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

YAG:Ce³⁺ nanoparticles 9.5∈±∈1.2 nm in diameter have been synthesized from aluminium isopropoxide and acetates of yttrium and cerium in 1,4-butanediol (1,4-BD) by autoclave treatment at 300 °C for 2 h. After replacing 1,4-BD by ultrapure water, NH₂ groups were introduced on the surface of YAG:Ce³⁺ nanoparticles by addition of 3-aminopropyltrimethoxysilane then biotinylation with sulfo-NHS-LC-biotin. We demonstrated that avidin immobilized beads are tagged by biotinylated YAG:Ce³⁺ nanoparticles by the selective avidin-biotin interaction, furnishing a green fluorescent image on excitation with blue light. This result indicates that YAG:Ce³⁺ nanoparticle phosphors have much potential in biological applications.

Original language	English
Pages (from-to)	1641-1647
Number of pages	7
Journal	Analytical and bioanalytical chemistry
Volume	386
Issue number	6
DOIs	https://doi.org/10.1007/s00216-006-0814-6
Publication status	Published - 2006 Nov

Keywords

3-aminopropyltrimethoxysilane
Avidin-biotin interaction
Biological application
Fluorescent image
Nanoparticle
YAG:Ce

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry

Access to Document

10.1007/s00216-006-0814-6

Cite this

@article{84176d329abd408ca8743895b780916a,

title = "Tagging of avidin immobilized beads with biotinylated YAG:Ce3+ nanocrystal phosphor",

abstract = "YAG:Ce3+ nanoparticles 9.5∈±∈1.2 nm in diameter have been synthesized from aluminium isopropoxide and acetates of yttrium and cerium in 1,4-butanediol (1,4-BD) by autoclave treatment at 300 °C for 2 h. After replacing 1,4-BD by ultrapure water, NH2 groups were introduced on the surface of YAG:Ce3+ nanoparticles by addition of 3-aminopropyltrimethoxysilane then biotinylation with sulfo-NHS-LC-biotin. We demonstrated that avidin immobilized beads are tagged by biotinylated YAG:Ce3+ nanoparticles by the selective avidin-biotin interaction, furnishing a green fluorescent image on excitation with blue light. This result indicates that YAG:Ce3+ nanoparticle phosphors have much potential in biological applications.",

keywords = "3-aminopropyltrimethoxysilane, Avidin-biotin interaction, Biological application, Fluorescent image, Nanoparticle, YAG:Ce",

author = "Ryo Asakura and Tetsuhiko Isobe and Kiyoshi Kurokawa and Hideki Aizawa and Michio Ohkubo",

note = "Funding Information: Acknowledgments This work was supported in part by the grant-in-aid from the New Energy and Industrial Technology Development Organization (NEDO). The authors thank Kasei Optonix Ltd for donating the reference sample.",

year = "2006",

month = nov,

doi = "10.1007/s00216-006-0814-6",

language = "English",

volume = "386",

pages = "1641--1647",

journal = "Fresenius Zeitschrift fur Analytische Chemie",

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AU - Asakura, Ryo

AU - Isobe, Tetsuhiko

AU - Kurokawa, Kiyoshi

AU - Aizawa, Hideki

AU - Ohkubo, Michio

N1 - Funding Information: Acknowledgments This work was supported in part by the grant-in-aid from the New Energy and Industrial Technology Development Organization (NEDO). The authors thank Kasei Optonix Ltd for donating the reference sample.

PY - 2006/11

Y1 - 2006/11

N2 - YAG:Ce3+ nanoparticles 9.5∈±∈1.2 nm in diameter have been synthesized from aluminium isopropoxide and acetates of yttrium and cerium in 1,4-butanediol (1,4-BD) by autoclave treatment at 300 °C for 2 h. After replacing 1,4-BD by ultrapure water, NH2 groups were introduced on the surface of YAG:Ce3+ nanoparticles by addition of 3-aminopropyltrimethoxysilane then biotinylation with sulfo-NHS-LC-biotin. We demonstrated that avidin immobilized beads are tagged by biotinylated YAG:Ce3+ nanoparticles by the selective avidin-biotin interaction, furnishing a green fluorescent image on excitation with blue light. This result indicates that YAG:Ce3+ nanoparticle phosphors have much potential in biological applications.

AB - YAG:Ce3+ nanoparticles 9.5∈±∈1.2 nm in diameter have been synthesized from aluminium isopropoxide and acetates of yttrium and cerium in 1,4-butanediol (1,4-BD) by autoclave treatment at 300 °C for 2 h. After replacing 1,4-BD by ultrapure water, NH2 groups were introduced on the surface of YAG:Ce3+ nanoparticles by addition of 3-aminopropyltrimethoxysilane then biotinylation with sulfo-NHS-LC-biotin. We demonstrated that avidin immobilized beads are tagged by biotinylated YAG:Ce3+ nanoparticles by the selective avidin-biotin interaction, furnishing a green fluorescent image on excitation with blue light. This result indicates that YAG:Ce3+ nanoparticle phosphors have much potential in biological applications.

KW - 3-aminopropyltrimethoxysilane

KW - Avidin-biotin interaction

KW - Biological application

KW - Fluorescent image

KW - Nanoparticle

KW - YAG:Ce

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