Tagging of avidin immobilized beads with biotinylated YAG:Ce3+ nanocrystal phosphor

Ryo Asakura, Tetsuhiko Isobe, Kiyoshi Kurokawa, Hideki Aizawa, Michio Ohkubo

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

YAG:Ce3+ nanoparticles 9.5∈±∈1.2 nm in diameter have been synthesized from aluminium isopropoxide and acetates of yttrium and cerium in 1,4-butanediol (1,4-BD) by autoclave treatment at 300 °C for 2 h. After replacing 1,4-BD by ultrapure water, NH2 groups were introduced on the surface of YAG:Ce3+ nanoparticles by addition of 3-aminopropyltrimethoxysilane then biotinylation with sulfo-NHS-LC-biotin. We demonstrated that avidin immobilized beads are tagged by biotinylated YAG:Ce3+ nanoparticles by the selective avidin-biotin interaction, furnishing a green fluorescent image on excitation with blue light. This result indicates that YAG:Ce3+ nanoparticle phosphors have much potential in biological applications.

Original languageEnglish
Pages (from-to)1641-1647
Number of pages7
JournalAnalytical and bioanalytical chemistry
Volume386
Issue number6
DOIs
Publication statusPublished - 2006 Nov 1

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Keywords

  • 3-aminopropyltrimethoxysilane
  • Avidin-biotin interaction
  • Biological application
  • Fluorescent image
  • Nanoparticle
  • YAG:Ce

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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