TAP/NXF1, the primary mRNA export receptor, specifically interacts with a neuronal RNA-binding protein HuD

Kuniaki Saito, Toshinobu Fujiwara, Jun Katahira, Kunio Inoue, Hiroshi Sakamoto

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Hu proteins are RNA-binding proteins that are implicated in the control of stabilization, nuclear export, and/or translation of specific mRNAs with AU-rich elements (AREs) in the 3′-untranslated region. Three neuron-specific Hu proteins (HuD, HuB, and HuC), but not a ubiquitously expressed Hu protein HuR, have an activity to induce neurite outgrowth when they are overexpressed in a rat neuronal cell line PC12. Here we show that TAP/NXF1, the primary export receptor for the bulk mRNA, is a specific binding partner for HuD. In vitro binding experiments using recombinant proteins revealed that the interaction between TAP and HuD is direct and that HuD can form a ternary complex together with both TAP and RNA. Interestingly, HuR does not interact with TAP. These results suggest that HuD acts as a novel adaptor protein to recruit TAP for efficient export of ARE-containing mRNAs in neuronal cells.

Original languageEnglish
Pages (from-to)291-297
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume321
Issue number2
DOIs
Publication statusPublished - 2004 Aug 20

Keywords

  • AU-rich element
  • HuD
  • Neuron
  • TAP
  • mRNA export

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'TAP/NXF1, the primary mRNA export receptor, specifically interacts with a neuronal RNA-binding protein HuD'. Together they form a unique fingerprint.

  • Cite this