Target DNA detection by solid-state nanopores using gold nanoparticle probes

Rena Akahori, Keiko Esashika, Itaru Yanagi, Toshiharu Saiki

Research output: Contribution to journalArticlepeer-review

Abstract

For target DNA detection with nanopores, the labeling of target DNA with large probes and the detection of its passage through a nanopore were examined. We labeled the target DNA with two gold nanoparticles (AuNPs). The surface of each AuNP was modified with a different single-stranded DNA (ssDNA) molecule that contained a complementary sequence to a different part of the target DNA so that the two AuNPs could capture one target DNA. The observed ionic current blockade when the AuNPs-labeled target DNA passed through a nanopore was significantly larger than that when nonlabeled DNA or a single AuNP probe passed through a nanopore. This result indicated that the presence of the target DNA in solution can be detected through nanopore measurements with two AuNP probes.

Original languageEnglish
Article number107001
JournalJapanese journal of applied physics
Volume59
Issue number10
DOIs
Publication statusPublished - 2020 Oct 1

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

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