Target-Selective Fluorescence Imaging and Photocytotoxicity against H2O2 High-Expressing Cancer Cells Using a Photoactivatable Theranostic Agent

Ryoma Takagi, Ayano Takeda, Daisuke Takahashi, Kazunobu Toshima

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A purpose-designed and synthesized H2O2-reactive and photoactivatable theranostic agent 1 consisting of 1)an arylboronic acid moiety, 2)pro-fluorophore moiety, and 3)photoactivatable moiety (photosensitizer), selectively and effectively reacted with H2O2 while simultaneously releasing resorufin for fluorescence detection under neutral aqueous conditions. In addition, 1 was cell-permeable, and exhibited effective photocytotoxicity against fluorescently visualized cells only upon photoirradiation. The results also showed that 1 produced a selective fluorescence response to H2O2, even in living cultured cells.

Original languageEnglish
JournalChemistry - An Asian Journal
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Optical Imaging
Fluorescence
Cells
Imaging techniques
Photosensitizing Agents
Fluorophores
Cultured Cells
Neoplasms
Acids
Theranostic Nanomedicine
resorufin

Keywords

  • Cytotoxicity
  • Fluorescent probes
  • Peroxides
  • Sensitizers
  • Theranostics

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

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title = "Target-Selective Fluorescence Imaging and Photocytotoxicity against H2O2 High-Expressing Cancer Cells Using a Photoactivatable Theranostic Agent",
abstract = "A purpose-designed and synthesized H2O2-reactive and photoactivatable theranostic agent 1 consisting of 1)an arylboronic acid moiety, 2)pro-fluorophore moiety, and 3)photoactivatable moiety (photosensitizer), selectively and effectively reacted with H2O2 while simultaneously releasing resorufin for fluorescence detection under neutral aqueous conditions. In addition, 1 was cell-permeable, and exhibited effective photocytotoxicity against fluorescently visualized cells only upon photoirradiation. The results also showed that 1 produced a selective fluorescence response to H2O2, even in living cultured cells.",
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T1 - Target-Selective Fluorescence Imaging and Photocytotoxicity against H2O2 High-Expressing Cancer Cells Using a Photoactivatable Theranostic Agent

AU - Takagi, Ryoma

AU - Takeda, Ayano

AU - Takahashi, Daisuke

AU - Toshima, Kazunobu

PY - 2017

Y1 - 2017

N2 - A purpose-designed and synthesized H2O2-reactive and photoactivatable theranostic agent 1 consisting of 1)an arylboronic acid moiety, 2)pro-fluorophore moiety, and 3)photoactivatable moiety (photosensitizer), selectively and effectively reacted with H2O2 while simultaneously releasing resorufin for fluorescence detection under neutral aqueous conditions. In addition, 1 was cell-permeable, and exhibited effective photocytotoxicity against fluorescently visualized cells only upon photoirradiation. The results also showed that 1 produced a selective fluorescence response to H2O2, even in living cultured cells.

AB - A purpose-designed and synthesized H2O2-reactive and photoactivatable theranostic agent 1 consisting of 1)an arylboronic acid moiety, 2)pro-fluorophore moiety, and 3)photoactivatable moiety (photosensitizer), selectively and effectively reacted with H2O2 while simultaneously releasing resorufin for fluorescence detection under neutral aqueous conditions. In addition, 1 was cell-permeable, and exhibited effective photocytotoxicity against fluorescently visualized cells only upon photoirradiation. The results also showed that 1 produced a selective fluorescence response to H2O2, even in living cultured cells.

KW - Cytotoxicity

KW - Fluorescent probes

KW - Peroxides

KW - Sensitizers

KW - Theranostics

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