Target-selective photo-degradation of a sialyl Lewis a (sLea) conjugate and photo-cytotoxicity against sLea positive cancer cells using an anthraquinone-antibody hybrid

Daisuke Takahashi, Takashi Nagao, Shota Sotokawa, Kazunobu Toshima

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A purpose-designed anthraquinone-monoclonal antibody (anti-sialyl Lewis A (sLea) mAb) hybrid 6 selectively bound to and effectively degraded the target glycoprotein, HSA (human serum albumin)-sLea conjugate 4. Degradation was achieved using long wavelength UV light irradiation in the absence of any additives and under neutral conditions. Furthermore, the hybrid 6 exhibited selective photo-cytotoxicity against the sLea positive cancer cells A431 and WiDr cells only upon photo-irradiation.

Original languageEnglish
Pages (from-to)1224-1228
Number of pages5
JournalMedChemComm
Volume7
Issue number6
DOIs
Publication statusPublished - 2016

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Anthraquinones
Photodegradation
Cytotoxicity
Cells
Irradiation
Antibodies
Ultraviolet Rays
Serum Albumin
Ultraviolet radiation
Neoplasms
Glycoproteins
Monoclonal Antibodies
Degradation
Wavelength
5-acetylneuraminyl-2-3-galactosyl-1-3-(fucopyranosyl-1-4)-N-acetylglucosamine

ASJC Scopus subject areas

  • Biochemistry
  • Pharmaceutical Science

Cite this

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abstract = "A purpose-designed anthraquinone-monoclonal antibody (anti-sialyl Lewis A (sLea) mAb) hybrid 6 selectively bound to and effectively degraded the target glycoprotein, HSA (human serum albumin)-sLea conjugate 4. Degradation was achieved using long wavelength UV light irradiation in the absence of any additives and under neutral conditions. Furthermore, the hybrid 6 exhibited selective photo-cytotoxicity against the sLea positive cancer cells A431 and WiDr cells only upon photo-irradiation.",
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AU - Takahashi, Daisuke

AU - Nagao, Takashi

AU - Sotokawa, Shota

AU - Toshima, Kazunobu

PY - 2016

Y1 - 2016

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AB - A purpose-designed anthraquinone-monoclonal antibody (anti-sialyl Lewis A (sLea) mAb) hybrid 6 selectively bound to and effectively degraded the target glycoprotein, HSA (human serum albumin)-sLea conjugate 4. Degradation was achieved using long wavelength UV light irradiation in the absence of any additives and under neutral conditions. Furthermore, the hybrid 6 exhibited selective photo-cytotoxicity against the sLea positive cancer cells A431 and WiDr cells only upon photo-irradiation.

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