Targeted DNA transfection into the mouse central nervous system using laser-induced stress waves

Yasushi Satoh, Yasunari Kanda, Mitsuhiro Terakawa, Minoru Obara, Katsushige Mizuno, Yasuhiro Watanabe, Shogo Endo, Hidetoshi Ooigawa, Hiroshi Nawashiro, Shunichi Sato, Kunio Takishima

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Abstract

We investigated the feasibility of gene transfer into the mouse central nervous system (CNS) by applying nanosecond pulsed laser-induced stress waves (LISWs). Intraventricular or hippocampal injection of a reporter gene [enhanced green fluorescent protein (EGFP)] followed by application of LISWs showed this method to be efficient in the CNS of newborn and adult mice. Cells expressing EGFP reside at least 3.5 mm from the surface of the tissue, while no apparent damage was detected. Additionally, expression of EGFP was limited to the area that was exposed to LISWs. Using this method, the formulation of plasmid DNA by cationic transfer reagent polyethylen-imine proved to be effective for improving transfer efficiency into the CNS.

Original languageEnglish
Article number060501
JournalJournal of Biomedical Optics
Volume10
Issue number6
DOIs
Publication statusPublished - 2005 Nov 1

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Keywords

  • Central nervous system
  • In vivo transfection
  • Laser-induced stress wave
  • Polyethylenimine
  • Stereo-taxic injection

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

Satoh, Y., Kanda, Y., Terakawa, M., Obara, M., Mizuno, K., Watanabe, Y., Endo, S., Ooigawa, H., Nawashiro, H., Sato, S., & Takishima, K. (2005). Targeted DNA transfection into the mouse central nervous system using laser-induced stress waves. Journal of Biomedical Optics, 10(6), [060501]. https://doi.org/10.1117/1.2128432