Abstract
The cricket (Gryllus bimaculatus) is a hemimetabolous insect that is emerging as a model organism for the study of neural and molecular mechanisms of behavioral traits. However, research strategies have been limited by a lack of genetic manipulation techniques that target the nervous system of the cricket. The development of a new method for efficient gene delivery into cricket brains, using in vivo electroporation, is described here. Plasmid DNA, which contained an enhanced green fluorescent protein (eGFP) gene, under the control of a G. bimaculatus actin (Gb'-act) promoter, was injected into adult cricket brains. Injection was followed by electroporation at a sufficient voltage. Expression of eGFP was observed within the brain tissue. Localized gene expression, targeted to specific regions of the brain, was also achieved using a combination of local DNA injection and fine arrangement of the electroporation electrodes. Further studies using this technique will lead to a better understanding of the neural and molecular mechanisms that underlie cricket behaviors.
| Original language | English |
|---|---|
| Pages (from-to) | 1235-1241 |
| Number of pages | 7 |
| Journal | Journal of Insect Physiology |
| Volume | 59 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2013 Dec |
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Keywords
- Brain
- Cricket
- Electroporation
- Green fluorescent protein
- Insect
- Neuron
ASJC Scopus subject areas
- Insect Science
- Physiology
Cite this
Targeted gene delivery in the cricket brain, using in vivo electroporation. / Matsumoto, Chihiro Sato; Shidara, Hisashi; Matsuda, Koji; Nakamura, Taro; Mito, Taro; Matsumoto, Yukihisa; Oka, Kotaro; Ogawa, Hiroto.
In: Journal of Insect Physiology, Vol. 59, No. 12, 12.2013, p. 1235-1241.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Targeted gene delivery in the cricket brain, using in vivo electroporation
AU - Matsumoto, Chihiro Sato
AU - Shidara, Hisashi
AU - Matsuda, Koji
AU - Nakamura, Taro
AU - Mito, Taro
AU - Matsumoto, Yukihisa
AU - Oka, Kotaro
AU - Ogawa, Hiroto
PY - 2013/12
Y1 - 2013/12
N2 - The cricket (Gryllus bimaculatus) is a hemimetabolous insect that is emerging as a model organism for the study of neural and molecular mechanisms of behavioral traits. However, research strategies have been limited by a lack of genetic manipulation techniques that target the nervous system of the cricket. The development of a new method for efficient gene delivery into cricket brains, using in vivo electroporation, is described here. Plasmid DNA, which contained an enhanced green fluorescent protein (eGFP) gene, under the control of a G. bimaculatus actin (Gb'-act) promoter, was injected into adult cricket brains. Injection was followed by electroporation at a sufficient voltage. Expression of eGFP was observed within the brain tissue. Localized gene expression, targeted to specific regions of the brain, was also achieved using a combination of local DNA injection and fine arrangement of the electroporation electrodes. Further studies using this technique will lead to a better understanding of the neural and molecular mechanisms that underlie cricket behaviors.
AB - The cricket (Gryllus bimaculatus) is a hemimetabolous insect that is emerging as a model organism for the study of neural and molecular mechanisms of behavioral traits. However, research strategies have been limited by a lack of genetic manipulation techniques that target the nervous system of the cricket. The development of a new method for efficient gene delivery into cricket brains, using in vivo electroporation, is described here. Plasmid DNA, which contained an enhanced green fluorescent protein (eGFP) gene, under the control of a G. bimaculatus actin (Gb'-act) promoter, was injected into adult cricket brains. Injection was followed by electroporation at a sufficient voltage. Expression of eGFP was observed within the brain tissue. Localized gene expression, targeted to specific regions of the brain, was also achieved using a combination of local DNA injection and fine arrangement of the electroporation electrodes. Further studies using this technique will lead to a better understanding of the neural and molecular mechanisms that underlie cricket behaviors.
KW - Brain
KW - Cricket
KW - Electroporation
KW - Green fluorescent protein
KW - Insect
KW - Neuron
UR - http://www.scopus.com/inward/record.url?scp=84887238778&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84887238778&partnerID=8YFLogxK
U2 - 10.1016/j.jinsphys.2013.10.001
DO - 10.1016/j.jinsphys.2013.10.001
M3 - Article
VL - 59
SP - 1235
EP - 1241
JO - Journal of Insect Physiology
JF - Journal of Insect Physiology
SN - 0022-1910
IS - 12
ER -