Targeted gene delivery in the cricket brain, using in vivo electroporation

Chihiro Sato Matsumoto, Hisashi Shidara, Koji Matsuda, Taro Nakamura, Taro Mito, Yukihisa Matsumoto, Kotaro Oka, Hiroto Ogawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The cricket (Gryllus bimaculatus) is a hemimetabolous insect that is emerging as a model organism for the study of neural and molecular mechanisms of behavioral traits. However, research strategies have been limited by a lack of genetic manipulation techniques that target the nervous system of the cricket. The development of a new method for efficient gene delivery into cricket brains, using in vivo electroporation, is described here. Plasmid DNA, which contained an enhanced green fluorescent protein (eGFP) gene, under the control of a G. bimaculatus actin (Gb'-act) promoter, was injected into adult cricket brains. Injection was followed by electroporation at a sufficient voltage. Expression of eGFP was observed within the brain tissue. Localized gene expression, targeted to specific regions of the brain, was also achieved using a combination of local DNA injection and fine arrangement of the electroporation electrodes. Further studies using this technique will lead to a better understanding of the neural and molecular mechanisms that underlie cricket behaviors.

Original languageEnglish
Pages (from-to)1235-1241
Number of pages7
JournalJournal of Insect Physiology
Volume59
Issue number12
DOIs
Publication statusPublished - 2013 Dec

Fingerprint

Gryllidae
Electroporation
electroporation
gene transfer
brain
Brain
Gryllus bimaculatus
Genes
green fluorescent protein
research planning
injection
Genetic Techniques
Injections
DNA
genetic engineering
nervous system
electrodes
Nervous System
actin
Insects

Keywords

  • Brain
  • Cricket
  • Electroporation
  • Green fluorescent protein
  • Insect
  • Neuron

ASJC Scopus subject areas

  • Insect Science
  • Physiology

Cite this

Matsumoto, C. S., Shidara, H., Matsuda, K., Nakamura, T., Mito, T., Matsumoto, Y., ... Ogawa, H. (2013). Targeted gene delivery in the cricket brain, using in vivo electroporation. Journal of Insect Physiology, 59(12), 1235-1241. https://doi.org/10.1016/j.jinsphys.2013.10.001

Targeted gene delivery in the cricket brain, using in vivo electroporation. / Matsumoto, Chihiro Sato; Shidara, Hisashi; Matsuda, Koji; Nakamura, Taro; Mito, Taro; Matsumoto, Yukihisa; Oka, Kotaro; Ogawa, Hiroto.

In: Journal of Insect Physiology, Vol. 59, No. 12, 12.2013, p. 1235-1241.

Research output: Contribution to journalArticle

Matsumoto, CS, Shidara, H, Matsuda, K, Nakamura, T, Mito, T, Matsumoto, Y, Oka, K & Ogawa, H 2013, 'Targeted gene delivery in the cricket brain, using in vivo electroporation', Journal of Insect Physiology, vol. 59, no. 12, pp. 1235-1241. https://doi.org/10.1016/j.jinsphys.2013.10.001
Matsumoto CS, Shidara H, Matsuda K, Nakamura T, Mito T, Matsumoto Y et al. Targeted gene delivery in the cricket brain, using in vivo electroporation. Journal of Insect Physiology. 2013 Dec;59(12):1235-1241. https://doi.org/10.1016/j.jinsphys.2013.10.001
Matsumoto, Chihiro Sato ; Shidara, Hisashi ; Matsuda, Koji ; Nakamura, Taro ; Mito, Taro ; Matsumoto, Yukihisa ; Oka, Kotaro ; Ogawa, Hiroto. / Targeted gene delivery in the cricket brain, using in vivo electroporation. In: Journal of Insect Physiology. 2013 ; Vol. 59, No. 12. pp. 1235-1241.
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