TY - JOUR
T1 - Targeted gene delivery in the cricket brain, using in vivo electroporation
AU - Matsumoto, Chihiro Sato
AU - Shidara, Hisashi
AU - Matsuda, Koji
AU - Nakamura, Taro
AU - Mito, Taro
AU - Matsumoto, Yukihisa
AU - Oka, Kotaro
AU - Ogawa, Hiroto
N1 - Funding Information:
We are grateful to Dr. N. Tanaka for helpful comments. This work was supported by JST, PRESTO to H.O.; JSPS and MEXT KAKENHI [ 24120502 to H.O.; 23687033 , 22124003 , and 22370080 to T.M.; #23111521 to T.N.]; MEXT Program for the Strategic Research Foundation at Private Universities [ S0801008 to K.O.].
PY - 2013/12
Y1 - 2013/12
N2 - The cricket (Gryllus bimaculatus) is a hemimetabolous insect that is emerging as a model organism for the study of neural and molecular mechanisms of behavioral traits. However, research strategies have been limited by a lack of genetic manipulation techniques that target the nervous system of the cricket. The development of a new method for efficient gene delivery into cricket brains, using in vivo electroporation, is described here. Plasmid DNA, which contained an enhanced green fluorescent protein (eGFP) gene, under the control of a G. bimaculatus actin (Gb'-act) promoter, was injected into adult cricket brains. Injection was followed by electroporation at a sufficient voltage. Expression of eGFP was observed within the brain tissue. Localized gene expression, targeted to specific regions of the brain, was also achieved using a combination of local DNA injection and fine arrangement of the electroporation electrodes. Further studies using this technique will lead to a better understanding of the neural and molecular mechanisms that underlie cricket behaviors.
AB - The cricket (Gryllus bimaculatus) is a hemimetabolous insect that is emerging as a model organism for the study of neural and molecular mechanisms of behavioral traits. However, research strategies have been limited by a lack of genetic manipulation techniques that target the nervous system of the cricket. The development of a new method for efficient gene delivery into cricket brains, using in vivo electroporation, is described here. Plasmid DNA, which contained an enhanced green fluorescent protein (eGFP) gene, under the control of a G. bimaculatus actin (Gb'-act) promoter, was injected into adult cricket brains. Injection was followed by electroporation at a sufficient voltage. Expression of eGFP was observed within the brain tissue. Localized gene expression, targeted to specific regions of the brain, was also achieved using a combination of local DNA injection and fine arrangement of the electroporation electrodes. Further studies using this technique will lead to a better understanding of the neural and molecular mechanisms that underlie cricket behaviors.
KW - Brain
KW - Cricket
KW - Electroporation
KW - Green fluorescent protein
KW - Insect
KW - Neuron
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U2 - 10.1016/j.jinsphys.2013.10.001
DO - 10.1016/j.jinsphys.2013.10.001
M3 - Article
C2 - 24161373
AN - SCOPUS:84887238778
VL - 59
SP - 1235
EP - 1241
JO - Journal of Insect Physiology
JF - Journal of Insect Physiology
SN - 0022-1910
IS - 12
ER -