Targeting delivery of therapeutic genes using monoclonal antibody; immunogene approach

A. Takayanagi, J. Chen, S. Gamou, N. Shimizu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We are developing the "immunogene" system for the targeted delivery of therapeutic genes. The immunogene system utilizes the EGF receptor-mediated endocytosis. The Fab fragment of monoclonal antibody B4G7 against human EGF receptor was conjugated with polylysine to form an "Fab immunoporter", which forms an affinity complex with DNA. The transfection efficiency of Fab immunogene was approximately 10-fold higher than the Lipofectin. Gene transfer of HSV-tk gene into A431 tumor cells with Fab immunoporter was successful and the subsequent treatment with ganciclovir induced remarkable suicide effects conferring 1000-fold higher drug sensitivity. Thus, the immunogene system could be useful as a gene transfer vehicle targeting the EGF receptor-hyperproducing tumor cells.

Original languageEnglish
Pages (from-to)731-736
Number of pages6
JournalNippon rinsho. Japanese journal of clinical medicine
Volume56
Issue number3
Publication statusPublished - 1998

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Monoclonal Antibodies
Epidermal Growth Factor Receptor
Genes
Immunoglobulin Fab Fragments
Ganciclovir
Polylysine
Therapeutics
Endocytosis
Suicide
Transfection
Neoplasms
DNA
Pharmaceutical Preparations

Cite this

Targeting delivery of therapeutic genes using monoclonal antibody; immunogene approach. / Takayanagi, A.; Chen, J.; Gamou, S.; Shimizu, N.

In: Nippon rinsho. Japanese journal of clinical medicine, Vol. 56, No. 3, 1998, p. 731-736.

Research output: Contribution to journalArticle

Takayanagi, A. ; Chen, J. ; Gamou, S. ; Shimizu, N. / Targeting delivery of therapeutic genes using monoclonal antibody; immunogene approach. In: Nippon rinsho. Japanese journal of clinical medicine. 1998 ; Vol. 56, No. 3. pp. 731-736.
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