Taurocholate induces directional excretion of bilirubin into bile in perfused rat liver

Tokio Yamaguchi, Yoshiyuki Wakabayashi, Makiko Tanaka, Tsuyoshi Sano, Hiromi Ishikawa, Hiroshi Nakajima, Makoto Suematsu, Yuzuru Ishimura

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The role of taurocholate, one of the major conjugated bile acids present in portal blood, in excretion of bilirubin from liver parenchyma to biliary and vascular compartments was studied in isolated perfused rat liver. Contents of bilirubin and carbon monoxide (CO) in the bile or venous effluents were determined by enzyme-linked immunosorbent microassay with the use of antibilirubin monoclonal antibody 24G7 and myoglobin-assisted spectrophotometry, respectively. In the presence of taurocholate, bilirubin excreted into the biliary compartment constituted greater than 90% of the total bilirubin excreted from the liver (0.26 nmol · min-1 · g liver- 1), corresponding to 60% of the outflow of CO into the venous effluents. In its absence, however, the total amount of bilirubin excreted into extrahepatic compartments was reduced to 27% of CO flux, and more than 90% of the excreted bilirubin was in the venous effluent. Thus a choleretic bile acid such as taurocholate is necessary for directional transport of bilirubin into bile in the perfused liver preparation.

Original languageEnglish
Pages (from-to)G1028-G1032
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume270
Issue number6 33-6
Publication statusPublished - 1996 Jun 1
Externally publishedYes

Keywords

  • biliary transport
  • carbon monoxide
  • heme oxygenase
  • hyperbilirubinemia

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

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