Taxonomic revision of oil-producing green algae, Chlorococcum oleofaciens (Volvocales, Chlorophyceae), and its relatives

Yuriko Kawasaki, Takashi Nakada, Masaru Tomita

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Historically, species in Volvocales were classified based primarily on morphology. Although the taxonomy of Chlamydomonas has been re-examined using a polyphasic approach including molecular phylogeny, that of Chlorococcum (Cc.), the largest coccoid genus in Volvocales, has yet to be reexamined. Six species thought to be synonymous with the oil-producing alga Cc. oleofaciens were previously not confirmed by molecular phylogeny. In this study, seven authentic strains of Cc. oleofaciens and its putative synonyms, along with 11 relatives, were examined based on the phylogeny of the 18S ribosomal RNA (rRNA) gene, comparisons of secondary structures of internal transcribed spacer 1 (ITS1) and ITS2 rDNA, and morphological observations by light microscopy. Seven 18S rRNA types were recognized among these strains and three were distantly related to Cc. oleofaciens. Comparisons of ITS rDNA structures suggested possible separation of the remaining four types into different species. Shapes of vegetative cells, thickness of the cell walls in old cultures, the size of cells in old cultures, and stigma morphology of zoospores also supported the 18S rRNA grouping. Based on these results, the 18 strains examined were reclassified into seven species. Among the putative synonyms, synonymy of Cc. oleofaciens, Cc. croceum, and Cc. granulosum was confirmed, and Cc. microstigmatum, Cc. rugosum, Cc. aquaticum, and Cc. nivale were distinguished from Cc. oleofaciens. Furthermore, another related strain is described as a new species, Macrochloris rubrioleum sp. nov.

Original languageEnglish
Pages (from-to)1000-1016
Number of pages17
JournalJournal of Phycology
Volume51
Issue number5
DOIs
Publication statusPublished - 2015 Oct 1

Fingerprint

Chlorococcum
Chlorophyceae
taxonomic revisions
green alga
Chlorophyta
oils
phylogeny
oil
RNA
secondary structure
microscopy
alga
new species
algae
Chlamydomonadales
gene
ribosomal RNA
Chlamydomonas
vegetative cells
zoospores

Keywords

  • Chlorococcum oleofaciens
  • ITS rDNA
  • 18S rRNA
  • Green algae
  • Molecular phylogeny
  • Morphology
  • Taxonomy

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

Taxonomic revision of oil-producing green algae, Chlorococcum oleofaciens (Volvocales, Chlorophyceae), and its relatives. / Kawasaki, Yuriko; Nakada, Takashi; Tomita, Masaru.

In: Journal of Phycology, Vol. 51, No. 5, 01.10.2015, p. 1000-1016.

Research output: Contribution to journalArticle

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