Tbx1 is regulated by forkhead proteins in the secondary heart field

Jun Maeda, Hiroyuki Yamagishi, John McAnally, Chihiro Yamagishi, Deepak Srivastava

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Transcriptional regulation in a tissue-specific and quantitative manner is essential for developmental events, including those involved in cardiovascular morphogenesis. Tbx1 is a T-box-containing transcription factor that is responsible for many of the defects observed in 22q11 deletion syndrome in humans. Tbx1 is expressed in the secondary heart field (SHF) and is essential for cardiac outflow tract (OFT) development. We previously reported that Tbx1 is regulated by sonic hedgehog by means of forkhead (Fox) transcription factors in the head mesenchyme and pharyngeal endoderm, but how it is regulated in the SHF is unknown. Here, we show that Tbx1 expression in the SHF is regulated by Fox proteins through a combination of two evolutionarily conserved Fox binding sites in a dose-dependent manner. Cell fate analysis using the Tbx1 enhancer suggests that SHF-derived Tbx1-expressing cells contribute extensively to the right ventricular myocardium as well as the OFT during early development and ultimately give rise to the right ventricular infundibulum, pulmonary trunk, and pulmonary valves. These results suggest that Fox proteins are involved in most, if not all, Tbx1 expression domains and that Tbx1 marks a subset of SHF-derived cells, particularly those that uniquely contribute to the right-sided outflow tract and proximal pulmonary artery.

Original languageEnglish
Pages (from-to)701-710
Number of pages10
JournalDevelopmental Dynamics
Volume235
Issue number3
DOIs
Publication statusPublished - 2006 Mar

Fingerprint

Forkhead Transcription Factors
22q11 Deletion Syndrome
Pulmonary Valve
Endoderm
Mesoderm
Pituitary Gland
Morphogenesis
Pulmonary Artery
Myocardium
Proteins
Transcription Factors
Binding Sites
Head
Lung

Keywords

  • Cardiac outflow tract
  • Fox proteins
  • Secondary heart field
  • Tbx1

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Tbx1 is regulated by forkhead proteins in the secondary heart field. / Maeda, Jun; Yamagishi, Hiroyuki; McAnally, John; Yamagishi, Chihiro; Srivastava, Deepak.

In: Developmental Dynamics, Vol. 235, No. 3, 03.2006, p. 701-710.

Research output: Contribution to journalArticle

Maeda, J, Yamagishi, H, McAnally, J, Yamagishi, C & Srivastava, D 2006, 'Tbx1 is regulated by forkhead proteins in the secondary heart field', Developmental Dynamics, vol. 235, no. 3, pp. 701-710. https://doi.org/10.1002/dvdy.20686
Maeda, Jun ; Yamagishi, Hiroyuki ; McAnally, John ; Yamagishi, Chihiro ; Srivastava, Deepak. / Tbx1 is regulated by forkhead proteins in the secondary heart field. In: Developmental Dynamics. 2006 ; Vol. 235, No. 3. pp. 701-710.
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