@article{7bbd1ff2a1b8418598f4a83c1f4bc2f7,
title = "TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients",
abstract = "Tumor draining lymph nodes (TDLNs) are located in the routes of lymphatic drainage from a primary tumor and have the highest risk of metastasis in various types of solid tumors. TDLNs are also considered as a tissue to activate the antitumor immunity, where antigen-specific effector T cells are generated. However, T cell receptor (TCR) repertoires in TDLNs have not been well characterized. We collected 23 colorectal cancer tumors with 203 lymph nodes with/without metastatic cancer cells (67 were metastasis-positive and the remaining 136 were metastasis-negative) and performed TCR sequencing. Metastasis-positive TDLNs showed a significantly lower TCR diversity and shared TCR clonotypes more frequently with primary tumor tissues compared to metastasis-negative TDLNs. Principal component analysis indicated that TDLNs with metastasis showed similar TCR repertoires. These findings suggest that cancer-reactive T cell clones could be enriched in the metastasis-positive TDLNs.",
keywords = "T cell receptor, colorectal cancer, immunogenomics, immunotherapy, lymph node",
author = "Tatsuo Matsuda and Eisaku Miyauchi and Hsu, {Yu Wen} and Satoshi Nagayama and Kazuma Kiyotani and Makda Zewde and Park, {Jae Hyun} and Taigo Kato and Makiko Harada and Shimpei Matsui and Masashi Ueno and Kazumasa Fukuda and Nobuaki Suzuki and Shoichi Hazama and Hiroaki Nagano and Hiroya Takeuchi and Vigneswaran, {Wickii T.} and Yuko Kitagawa and Yusuke Nakamura",
note = "Funding Information: aDepartment of Medicine, The University of Chicago, Chicago, IL, USA; bDepartment of Surgery, Keio University School of Medicine, Tokyo, Japan; cDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan; dThe PhD Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University and Academia Sinica, Taipei, Taiwan; eDepartment of Gastroenterological Surgery, Gastroenterological Center, Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, Japan; fCancer Precision Medicine Center, Japanese Foundation for Cancer Research, Tokyo, Japan; gDepartment of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan; hDepartment of Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan; iDepartment of Thoracic and Cardiovascular Surgery, Loyola University Medical Center, Maywood, IL, USA Funding Information: This work was supported by the OncoTherapy Science Inc.",
year = "2019",
month = jun,
day = "3",
doi = "10.1080/2162402X.2019.1588085",
language = "English",
volume = "8",
journal = "OncoImmunology",
issn = "2162-4011",
publisher = "Landes Bioscience",
number = "6",
}