TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients

Tatsuo Matsuda; Eisaku Miyauchi; Yu Wen Hsu; Satoshi Nagayama; Kazuma Kiyotani; Makda Zewde; Jae Hyun Park; Taigo Kato; Makiko Harada; Shimpei Matsui; Masashi Ueno; Kazumasa Fukuda; Nobuaki Suzuki; Shoichi Hazama; Hiroaki Nagano; Hiroya Takeuchi; Wickii T. Vigneswaran; Yuukou Kitagawa; Yusuke Nakamura

doi:10.1080/2162402X.2019.1588085

TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients

Tatsuo Matsuda, Eisaku Miyauchi, Yu Wen Hsu, Satoshi Nagayama, Kazuma Kiyotani, Makda Zewde, Jae Hyun Park, Taigo Kato, Makiko Harada, Shimpei Matsui, Masashi Ueno, Kazumasa Fukuda, Nobuaki Suzuki, Shoichi Hazama, Hiroaki Nagano, Hiroya Takeuchi, Wickii T. Vigneswaran, Yuukou Kitagawa, Yusuke Nakamura

Department of Surgery (General and Gastroenterological Surgery)

Research output: Contribution to journal › Article

Abstract

Tumor draining lymph nodes (TDLNs) are located in the routes of lymphatic drainage from a primary tumor and have the highest risk of metastasis in various types of solid tumors. TDLNs are also considered as a tissue to activate the antitumor immunity, where antigen-specific effector T cells are generated. However, T cell receptor (TCR) repertoires in TDLNs have not been well characterized. We collected 23 colorectal cancer tumors with 203 lymph nodes with/without metastatic cancer cells (67 were metastasis-positive and the remaining 136 were metastasis-negative) and performed TCR sequencing. Metastasis-positive TDLNs showed a significantly lower TCR diversity and shared TCR clonotypes more frequently with primary tumor tissues compared to metastasis-negative TDLNs. Principal component analysis indicated that TDLNs with metastasis showed similar TCR repertoires. These findings suggest that cancer-reactive T cell clones could be enriched in the metastasis-positive TDLNs.

Original language	English
Article number	e1588085
Journal	OncoImmunology
Volume	8
Issue number	6
DOIs	https://doi.org/10.1080/2162402X.2019.1588085
Publication status	Published - 2019 Jun 3

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Keywords

colorectal cancer
immunogenomics
immunotherapy
lymph node
T cell receptor

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Oncology

Cite this

Matsuda, T., Miyauchi, E., Hsu, Y. W., Nagayama, S., Kiyotani, K., Zewde, M., Park, J. H., Kato, T., Harada, M., Matsui, S., Ueno, M., Fukuda, K., Suzuki, N., Hazama, S., Nagano, H., Takeuchi, H., Vigneswaran, W. T., Kitagawa, Y., & Nakamura, Y. (2019). TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients. OncoImmunology, 8(6), [e1588085]. https://doi.org/10.1080/2162402X.2019.1588085

TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients. / Matsuda, Tatsuo; Miyauchi, Eisaku; Hsu, Yu Wen; Nagayama, Satoshi; Kiyotani, Kazuma; Zewde, Makda; Park, Jae Hyun; Kato, Taigo; Harada, Makiko; Matsui, Shimpei; Ueno, Masashi; Fukuda, Kazumasa; Suzuki, Nobuaki; Hazama, Shoichi; Nagano, Hiroaki; Takeuchi, Hiroya; Vigneswaran, Wickii T.; Kitagawa, Yuukou; Nakamura, Yusuke.

In: OncoImmunology, Vol. 8, No. 6, e1588085, 03.06.2019.

Research output: Contribution to journal › Article

Matsuda, T, Miyauchi, E, Hsu, YW, Nagayama, S, Kiyotani, K, Zewde, M, Park, JH, Kato, T, Harada, M, Matsui, S, Ueno, M, Fukuda, K, Suzuki, N, Hazama, S, Nagano, H, Takeuchi, H, Vigneswaran, WT, Kitagawa, Y & Nakamura, Y 2019, 'TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients', OncoImmunology, vol. 8, no. 6, e1588085. https://doi.org/10.1080/2162402X.2019.1588085

Matsuda, Tatsuo ; Miyauchi, Eisaku ; Hsu, Yu Wen ; Nagayama, Satoshi ; Kiyotani, Kazuma ; Zewde, Makda ; Park, Jae Hyun ; Kato, Taigo ; Harada, Makiko ; Matsui, Shimpei ; Ueno, Masashi ; Fukuda, Kazumasa ; Suzuki, Nobuaki ; Hazama, Shoichi ; Nagano, Hiroaki ; Takeuchi, Hiroya ; Vigneswaran, Wickii T. ; Kitagawa, Yuukou ; Nakamura, Yusuke. / TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients. In: OncoImmunology. 2019 ; Vol. 8, No. 6.

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AU - Nagano, Hiroaki

AU - Takeuchi, Hiroya

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10.1080/2162402X.2019.1588085