Abstract
Tumor draining lymph nodes (TDLNs) are located in the routes of lymphatic drainage from a primary tumor and have the highest risk of metastasis in various types of solid tumors. TDLNs are also considered as a tissue to activate the antitumor immunity, where antigen-specific effector T cells are generated. However, T cell receptor (TCR) repertoires in TDLNs have not been well characterized. We collected 23 colorectal cancer tumors with 203 lymph nodes with/without metastatic cancer cells (67 were metastasis-positive and the remaining 136 were metastasis-negative) and performed TCR sequencing. Metastasis-positive TDLNs showed a significantly lower TCR diversity and shared TCR clonotypes more frequently with primary tumor tissues compared to metastasis-negative TDLNs. Principal component analysis indicated that TDLNs with metastasis showed similar TCR repertoires. These findings suggest that cancer-reactive T cell clones could be enriched in the metastasis-positive TDLNs.
| Original language | English |
|---|---|
| Article number | e1588085 |
| Journal | OncoImmunology |
| Volume | 8 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2019 Jun 3 |
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Keywords
- colorectal cancer
- immunogenomics
- immunotherapy
- lymph node
- T cell receptor
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Oncology
Cite this
TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients. / Matsuda, Tatsuo; Miyauchi, Eisaku; Hsu, Yu Wen; Nagayama, Satoshi; Kiyotani, Kazuma; Zewde, Makda; Park, Jae Hyun; Kato, Taigo; Harada, Makiko; Matsui, Shimpei; Ueno, Masashi; Fukuda, Kazumasa; Suzuki, Nobuaki; Hazama, Shoichi; Nagano, Hiroaki; Takeuchi, Hiroya; Vigneswaran, Wickii T.; Kitagawa, Yuukou; Nakamura, Yusuke.
In: OncoImmunology, Vol. 8, No. 6, e1588085, 03.06.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients
AU - Matsuda, Tatsuo
AU - Miyauchi, Eisaku
AU - Hsu, Yu Wen
AU - Nagayama, Satoshi
AU - Kiyotani, Kazuma
AU - Zewde, Makda
AU - Park, Jae Hyun
AU - Kato, Taigo
AU - Harada, Makiko
AU - Matsui, Shimpei
AU - Ueno, Masashi
AU - Fukuda, Kazumasa
AU - Suzuki, Nobuaki
AU - Hazama, Shoichi
AU - Nagano, Hiroaki
AU - Takeuchi, Hiroya
AU - Vigneswaran, Wickii T.
AU - Kitagawa, Yuukou
AU - Nakamura, Yusuke
PY - 2019/6/3
Y1 - 2019/6/3
N2 - Tumor draining lymph nodes (TDLNs) are located in the routes of lymphatic drainage from a primary tumor and have the highest risk of metastasis in various types of solid tumors. TDLNs are also considered as a tissue to activate the antitumor immunity, where antigen-specific effector T cells are generated. However, T cell receptor (TCR) repertoires in TDLNs have not been well characterized. We collected 23 colorectal cancer tumors with 203 lymph nodes with/without metastatic cancer cells (67 were metastasis-positive and the remaining 136 were metastasis-negative) and performed TCR sequencing. Metastasis-positive TDLNs showed a significantly lower TCR diversity and shared TCR clonotypes more frequently with primary tumor tissues compared to metastasis-negative TDLNs. Principal component analysis indicated that TDLNs with metastasis showed similar TCR repertoires. These findings suggest that cancer-reactive T cell clones could be enriched in the metastasis-positive TDLNs.
AB - Tumor draining lymph nodes (TDLNs) are located in the routes of lymphatic drainage from a primary tumor and have the highest risk of metastasis in various types of solid tumors. TDLNs are also considered as a tissue to activate the antitumor immunity, where antigen-specific effector T cells are generated. However, T cell receptor (TCR) repertoires in TDLNs have not been well characterized. We collected 23 colorectal cancer tumors with 203 lymph nodes with/without metastatic cancer cells (67 were metastasis-positive and the remaining 136 were metastasis-negative) and performed TCR sequencing. Metastasis-positive TDLNs showed a significantly lower TCR diversity and shared TCR clonotypes more frequently with primary tumor tissues compared to metastasis-negative TDLNs. Principal component analysis indicated that TDLNs with metastasis showed similar TCR repertoires. These findings suggest that cancer-reactive T cell clones could be enriched in the metastasis-positive TDLNs.
KW - colorectal cancer
KW - immunogenomics
KW - immunotherapy
KW - lymph node
KW - T cell receptor
UR - http://www.scopus.com/inward/record.url?scp=85063196215&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85063196215&partnerID=8YFLogxK
U2 - 10.1080/2162402X.2019.1588085
DO - 10.1080/2162402X.2019.1588085
M3 - Article
AN - SCOPUS:85063196215
VL - 8
JO - OncoImmunology
JF - OncoImmunology
SN - 2162-4011
IS - 6
M1 - e1588085
ER -