TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients

Tatsuo Matsuda; Eisaku Miyauchi; Yu Wen Hsu; Satoshi Nagayama; Kazuma Kiyotani; Makda Zewde; Jae Hyun Park; Taigo Kato; Makiko Harada; Shimpei Matsui; Masashi Ueno; Kazumasa Fukuda; Nobuaki Suzuki; Shoichi Hazama; Hiroaki Nagano; Hiroya Takeuchi; Wickii T. Vigneswaran; Yuko Kitagawa; Yusuke Nakamura

doi:10.1080/2162402X.2019.1588085

TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients

Tatsuo Matsuda, Eisaku Miyauchi, Yu Wen Hsu, Satoshi Nagayama, Kazuma Kiyotani, Makda Zewde, Jae Hyun Park, Taigo Kato, Makiko Harada, Shimpei Matsui, Masashi Ueno, Kazumasa Fukuda, Nobuaki Suzuki, Shoichi Hazama, Hiroaki Nagano, Hiroya Takeuchi, Wickii T. Vigneswaran, Yuko Kitagawa, Yusuke Nakamura

Department of Surgery (General and Gastroenterological Surgery)

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Tumor draining lymph nodes (TDLNs) are located in the routes of lymphatic drainage from a primary tumor and have the highest risk of metastasis in various types of solid tumors. TDLNs are also considered as a tissue to activate the antitumor immunity, where antigen-specific effector T cells are generated. However, T cell receptor (TCR) repertoires in TDLNs have not been well characterized. We collected 23 colorectal cancer tumors with 203 lymph nodes with/without metastatic cancer cells (67 were metastasis-positive and the remaining 136 were metastasis-negative) and performed TCR sequencing. Metastasis-positive TDLNs showed a significantly lower TCR diversity and shared TCR clonotypes more frequently with primary tumor tissues compared to metastasis-negative TDLNs. Principal component analysis indicated that TDLNs with metastasis showed similar TCR repertoires. These findings suggest that cancer-reactive T cell clones could be enriched in the metastasis-positive TDLNs.

Original language	English
Article number	e1588085
Journal	OncoImmunology
Volume	8
Issue number	6
DOIs	https://doi.org/10.1080/2162402X.2019.1588085
Publication status	Published - 2019 Jun 3

Keywords

T cell receptor
colorectal cancer
immunogenomics
immunotherapy
lymph node

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Oncology

Access to Document

10.1080/2162402X.2019.1588085

Fingerprint Dive into the research topics of 'TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients'. Together they form a unique fingerprint.

Cite this

Matsuda, T., Miyauchi, E., Hsu, Y. W., Nagayama, S., Kiyotani, K., Zewde, M., Park, J. H., Kato, T., Harada, M., Matsui, S., Ueno, M., Fukuda, K., Suzuki, N., Hazama, S., Nagano, H., Takeuchi, H., Vigneswaran, W. T., Kitagawa, Y., & Nakamura, Y. (2019). TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients. OncoImmunology, 8(6), [e1588085]. https://doi.org/10.1080/2162402X.2019.1588085

TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients. / Matsuda, Tatsuo; Miyauchi, Eisaku; Hsu, Yu Wen; Nagayama, Satoshi; Kiyotani, Kazuma; Zewde, Makda; Park, Jae Hyun; Kato, Taigo; Harada, Makiko; Matsui, Shimpei; Ueno, Masashi; Fukuda, Kazumasa; Suzuki, Nobuaki; Hazama, Shoichi; Nagano, Hiroaki; Takeuchi, Hiroya; Vigneswaran, Wickii T.; Kitagawa, Yuko; Nakamura, Yusuke.

In: OncoImmunology, Vol. 8, No. 6, e1588085, 03.06.2019.

Research output: Contribution to journal › Article › peer-review

Matsuda, T, Miyauchi, E, Hsu, YW, Nagayama, S, Kiyotani, K, Zewde, M, Park, JH, Kato, T, Harada, M, Matsui, S, Ueno, M, Fukuda, K, Suzuki, N, Hazama, S, Nagano, H, Takeuchi, H, Vigneswaran, WT, Kitagawa, Y & Nakamura, Y 2019, 'TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients', OncoImmunology, vol. 8, no. 6, e1588085. https://doi.org/10.1080/2162402X.2019.1588085

Matsuda, Tatsuo ; Miyauchi, Eisaku ; Hsu, Yu Wen ; Nagayama, Satoshi ; Kiyotani, Kazuma ; Zewde, Makda ; Park, Jae Hyun ; Kato, Taigo ; Harada, Makiko ; Matsui, Shimpei ; Ueno, Masashi ; Fukuda, Kazumasa ; Suzuki, Nobuaki ; Hazama, Shoichi ; Nagano, Hiroaki ; Takeuchi, Hiroya ; Vigneswaran, Wickii T. ; Kitagawa, Yuko ; Nakamura, Yusuke. / TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients. In: OncoImmunology. 2019 ; Vol. 8, No. 6.

@article{7bbd1ff2a1b8418598f4a83c1f4bc2f7,

title = "TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients",

abstract = "Tumor draining lymph nodes (TDLNs) are located in the routes of lymphatic drainage from a primary tumor and have the highest risk of metastasis in various types of solid tumors. TDLNs are also considered as a tissue to activate the antitumor immunity, where antigen-specific effector T cells are generated. However, T cell receptor (TCR) repertoires in TDLNs have not been well characterized. We collected 23 colorectal cancer tumors with 203 lymph nodes with/without metastatic cancer cells (67 were metastasis-positive and the remaining 136 were metastasis-negative) and performed TCR sequencing. Metastasis-positive TDLNs showed a significantly lower TCR diversity and shared TCR clonotypes more frequently with primary tumor tissues compared to metastasis-negative TDLNs. Principal component analysis indicated that TDLNs with metastasis showed similar TCR repertoires. These findings suggest that cancer-reactive T cell clones could be enriched in the metastasis-positive TDLNs.",

keywords = "T cell receptor, colorectal cancer, immunogenomics, immunotherapy, lymph node",

author = "Tatsuo Matsuda and Eisaku Miyauchi and Hsu, {Yu Wen} and Satoshi Nagayama and Kazuma Kiyotani and Makda Zewde and Park, {Jae Hyun} and Taigo Kato and Makiko Harada and Shimpei Matsui and Masashi Ueno and Kazumasa Fukuda and Nobuaki Suzuki and Shoichi Hazama and Hiroaki Nagano and Hiroya Takeuchi and Vigneswaran, {Wickii T.} and Yuko Kitagawa and Yusuke Nakamura",

note = "Funding Information: aDepartment of Medicine, The University of Chicago, Chicago, IL, USA; bDepartment of Surgery, Keio University School of Medicine, Tokyo, Japan; cDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan; dThe PhD Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University and Academia Sinica, Taipei, Taiwan; eDepartment of Gastroenterological Surgery, Gastroenterological Center, Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, Japan; fCancer Precision Medicine Center, Japanese Foundation for Cancer Research, Tokyo, Japan; gDepartment of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan; hDepartment of Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan; iDepartment of Thoracic and Cardiovascular Surgery, Loyola University Medical Center, Maywood, IL, USA Funding Information: This work was supported by the OncoTherapy Science Inc.",

year = "2019",

month = jun,

day = "3",

doi = "10.1080/2162402X.2019.1588085",

language = "English",

volume = "8",

journal = "OncoImmunology",

issn = "2162-4011",

publisher = "Landes Bioscience",

number = "6",

}

TY - JOUR

T1 - TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients

AU - Matsuda, Tatsuo

AU - Miyauchi, Eisaku

AU - Hsu, Yu Wen

AU - Nagayama, Satoshi

AU - Kiyotani, Kazuma

AU - Zewde, Makda

AU - Park, Jae Hyun

AU - Kato, Taigo

AU - Harada, Makiko

AU - Matsui, Shimpei

AU - Ueno, Masashi

AU - Fukuda, Kazumasa

AU - Suzuki, Nobuaki

AU - Hazama, Shoichi

AU - Nagano, Hiroaki

AU - Takeuchi, Hiroya

AU - Vigneswaran, Wickii T.

AU - Kitagawa, Yuko

AU - Nakamura, Yusuke

N1 - Funding Information: aDepartment of Medicine, The University of Chicago, Chicago, IL, USA; bDepartment of Surgery, Keio University School of Medicine, Tokyo, Japan; cDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan; dThe PhD Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University and Academia Sinica, Taipei, Taiwan; eDepartment of Gastroenterological Surgery, Gastroenterological Center, Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, Japan; fCancer Precision Medicine Center, Japanese Foundation for Cancer Research, Tokyo, Japan; gDepartment of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan; hDepartment of Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan; iDepartment of Thoracic and Cardiovascular Surgery, Loyola University Medical Center, Maywood, IL, USA Funding Information: This work was supported by the OncoTherapy Science Inc.

PY - 2019/6/3

Y1 - 2019/6/3

N2 - Tumor draining lymph nodes (TDLNs) are located in the routes of lymphatic drainage from a primary tumor and have the highest risk of metastasis in various types of solid tumors. TDLNs are also considered as a tissue to activate the antitumor immunity, where antigen-specific effector T cells are generated. However, T cell receptor (TCR) repertoires in TDLNs have not been well characterized. We collected 23 colorectal cancer tumors with 203 lymph nodes with/without metastatic cancer cells (67 were metastasis-positive and the remaining 136 were metastasis-negative) and performed TCR sequencing. Metastasis-positive TDLNs showed a significantly lower TCR diversity and shared TCR clonotypes more frequently with primary tumor tissues compared to metastasis-negative TDLNs. Principal component analysis indicated that TDLNs with metastasis showed similar TCR repertoires. These findings suggest that cancer-reactive T cell clones could be enriched in the metastasis-positive TDLNs.

AB - Tumor draining lymph nodes (TDLNs) are located in the routes of lymphatic drainage from a primary tumor and have the highest risk of metastasis in various types of solid tumors. TDLNs are also considered as a tissue to activate the antitumor immunity, where antigen-specific effector T cells are generated. However, T cell receptor (TCR) repertoires in TDLNs have not been well characterized. We collected 23 colorectal cancer tumors with 203 lymph nodes with/without metastatic cancer cells (67 were metastasis-positive and the remaining 136 were metastasis-negative) and performed TCR sequencing. Metastasis-positive TDLNs showed a significantly lower TCR diversity and shared TCR clonotypes more frequently with primary tumor tissues compared to metastasis-negative TDLNs. Principal component analysis indicated that TDLNs with metastasis showed similar TCR repertoires. These findings suggest that cancer-reactive T cell clones could be enriched in the metastasis-positive TDLNs.

KW - T cell receptor

KW - colorectal cancer

KW - immunogenomics

KW - immunotherapy

KW - lymph node

UR - http://www.scopus.com/inward/record.url?scp=85063196215&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063196215&partnerID=8YFLogxK

U2 - 10.1080/2162402X.2019.1588085

DO - 10.1080/2162402X.2019.1588085

M3 - Article

AN - SCOPUS:85063196215

VL - 8

JO - OncoImmunology

JF - OncoImmunology

SN - 2162-4011

IS - 6

M1 - e1588085

ER -