TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients

Tatsuo Matsuda, Eisaku Miyauchi, Yu Wen Hsu, Satoshi Nagayama, Kazuma Kiyotani, Makda Zewde, Jae Hyun Park, Taigo Kato, Makiko Harada, Shimpei Matsui, Masashi Ueno, Kazumasa Fukuda, Nobuaki Suzuki, Shoichi Hazama, Hiroaki Nagano, Hiroya Takeuchi, Wickii T. Vigneswaran, Yuukou Kitagawa, Yusuke Nakamura

Research output: Contribution to journalArticle

Abstract

Tumor draining lymph nodes (TDLNs) are located in the routes of lymphatic drainage from a primary tumor and have the highest risk of metastasis in various types of solid tumors. TDLNs are also considered as a tissue to activate the antitumor immunity, where antigen-specific effector T cells are generated. However, T cell receptor (TCR) repertoires in TDLNs have not been well characterized. We collected 23 colorectal cancer tumors with 203 lymph nodes with/without metastatic cancer cells (67 were metastasis-positive and the remaining 136 were metastasis-negative) and performed TCR sequencing. Metastasis-positive TDLNs showed a significantly lower TCR diversity and shared TCR clonotypes more frequently with primary tumor tissues compared to metastasis-negative TDLNs. Principal component analysis indicated that TDLNs with metastasis showed similar TCR repertoires. These findings suggest that cancer-reactive T cell clones could be enriched in the metastasis-positive TDLNs.

Original languageEnglish
Article numbere1588085
JournalOncoImmunology
Volume8
Issue number6
DOIs
Publication statusPublished - 2019 Jun 3

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T-Cell Antigen Receptor
Colorectal Neoplasms
Lymph Nodes
Neoplasms
Neoplasm Metastasis
T-Lymphocytes
Principal Component Analysis
Drainage
Immunity
Clone Cells
Antigens

Keywords

  • colorectal cancer
  • immunogenomics
  • immunotherapy
  • lymph node
  • T cell receptor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology

Cite this

Matsuda, T., Miyauchi, E., Hsu, Y. W., Nagayama, S., Kiyotani, K., Zewde, M., ... Nakamura, Y. (2019). TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients. OncoImmunology, 8(6), [e1588085]. https://doi.org/10.1080/2162402X.2019.1588085

TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients. / Matsuda, Tatsuo; Miyauchi, Eisaku; Hsu, Yu Wen; Nagayama, Satoshi; Kiyotani, Kazuma; Zewde, Makda; Park, Jae Hyun; Kato, Taigo; Harada, Makiko; Matsui, Shimpei; Ueno, Masashi; Fukuda, Kazumasa; Suzuki, Nobuaki; Hazama, Shoichi; Nagano, Hiroaki; Takeuchi, Hiroya; Vigneswaran, Wickii T.; Kitagawa, Yuukou; Nakamura, Yusuke.

In: OncoImmunology, Vol. 8, No. 6, e1588085, 03.06.2019.

Research output: Contribution to journalArticle

Matsuda, T, Miyauchi, E, Hsu, YW, Nagayama, S, Kiyotani, K, Zewde, M, Park, JH, Kato, T, Harada, M, Matsui, S, Ueno, M, Fukuda, K, Suzuki, N, Hazama, S, Nagano, H, Takeuchi, H, Vigneswaran, WT, Kitagawa, Y & Nakamura, Y 2019, 'TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients', OncoImmunology, vol. 8, no. 6, e1588085. https://doi.org/10.1080/2162402X.2019.1588085
Matsuda, Tatsuo ; Miyauchi, Eisaku ; Hsu, Yu Wen ; Nagayama, Satoshi ; Kiyotani, Kazuma ; Zewde, Makda ; Park, Jae Hyun ; Kato, Taigo ; Harada, Makiko ; Matsui, Shimpei ; Ueno, Masashi ; Fukuda, Kazumasa ; Suzuki, Nobuaki ; Hazama, Shoichi ; Nagano, Hiroaki ; Takeuchi, Hiroya ; Vigneswaran, Wickii T. ; Kitagawa, Yuukou ; Nakamura, Yusuke. / TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients. In: OncoImmunology. 2019 ; Vol. 8, No. 6.
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