Tear cytokine and ocular surface alterations following brief passive cigarette smoke exposure

Van Tuan Rummenie, Yukihiro Matsumoto, Murat Dogru, Yan Wang, Yiqian Hu, Samantha K. Ward, Ayako Igarashi, Tais Wakamatsu, Osama Ibrahim, Eiki Goto, Gré Luyten, Hiroko Inoue, Ichiro Saito, Jun Shimazaki, Kazuo Tsubota

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Purpose: To prospectively investigate the effects of acute passive cigarette smoke exposure on the ocular surface and tear film in healthy non-smokers. Methods: Twelve right eyes of 12 subjects without any ocular diseases were examined before, 5 min, and 24 h after 5 min of passive cigarette smoke exposure in a controlled smoke chamber. Tear samples were obtained before, 5 min and 24 h after smoke exposure to detect tear hexanoyl-lysine (HEL), acrolein and inflammatory cytokine concentrations. Tear evaporation rate, DR-1 tear film lipid layer interferometry, tear film break-up time (TBUT), ocular surface fluorescein staining (FS) and Rose Bengal staining (RB), Schirmer I test were performed before, 5 min, and 24 h after smoke exposure. Conjunctival impression cytology (IC) and brush cytology (BC) were carried out before and 24 h after smoke exposure. Results: Tear evaporation rate, tear lipid spread time, tear film break-up time, and vital staining scores showed significant worsening with passive smoke exposure. Tear HEL and IL-6 concentrations increased significantly 24 h after smoke exposure. Tear acrolein level showed an insignificant increase at 5 min. IC and RT-PCR revealed a significant reduction in goblet cell density, a shift toward higher squamous metaplasia grades and a significant downregulation of MUC5AC mRNA expression at 24 h. Conclusion: Even brief passive exposure to cigarette smoke in healthy non-smoker subjects was associated with adverse effects on the ocular surface health as evidenced by an increase of tear inflammatory cytokines, tear lipid peroxidation products and decrease of mucosal defense resulting in tear instability and damage to the ocular surface epithelia.

Original languageEnglish
Pages (from-to)200-208
Number of pages9
JournalCytokine
Volume43
Issue number2
DOIs
Publication statusPublished - 2008 Aug

Fingerprint

Tears
Smoke
Tobacco Products
Cytokines
Cytology
Acrolein
Lipids
Lysine
Cell Biology
Evaporation
Staining and Labeling
Rose Bengal
Brushes
Fluorescein
Interferometry
Interleukin-6
Goblet Cells
Eye Diseases
Health
Metaplasia

Keywords

  • Cytokine
  • Dry eye
  • Hexanoyl-lysine
  • Mucin
  • Smoking

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Tear cytokine and ocular surface alterations following brief passive cigarette smoke exposure. / Rummenie, Van Tuan; Matsumoto, Yukihiro; Dogru, Murat; Wang, Yan; Hu, Yiqian; Ward, Samantha K.; Igarashi, Ayako; Wakamatsu, Tais; Ibrahim, Osama; Goto, Eiki; Luyten, Gré; Inoue, Hiroko; Saito, Ichiro; Shimazaki, Jun; Tsubota, Kazuo.

In: Cytokine, Vol. 43, No. 2, 08.2008, p. 200-208.

Research output: Contribution to journalArticle

Rummenie, VT, Matsumoto, Y, Dogru, M, Wang, Y, Hu, Y, Ward, SK, Igarashi, A, Wakamatsu, T, Ibrahim, O, Goto, E, Luyten, G, Inoue, H, Saito, I, Shimazaki, J & Tsubota, K 2008, 'Tear cytokine and ocular surface alterations following brief passive cigarette smoke exposure', Cytokine, vol. 43, no. 2, pp. 200-208. https://doi.org/10.1016/j.cyto.2008.05.011
Rummenie, Van Tuan ; Matsumoto, Yukihiro ; Dogru, Murat ; Wang, Yan ; Hu, Yiqian ; Ward, Samantha K. ; Igarashi, Ayako ; Wakamatsu, Tais ; Ibrahim, Osama ; Goto, Eiki ; Luyten, Gré ; Inoue, Hiroko ; Saito, Ichiro ; Shimazaki, Jun ; Tsubota, Kazuo. / Tear cytokine and ocular surface alterations following brief passive cigarette smoke exposure. In: Cytokine. 2008 ; Vol. 43, No. 2. pp. 200-208.
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AU - Rummenie, Van Tuan

AU - Matsumoto, Yukihiro

AU - Dogru, Murat

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AU - Hu, Yiqian

AU - Ward, Samantha K.

AU - Igarashi, Ayako

AU - Wakamatsu, Tais

AU - Ibrahim, Osama

AU - Goto, Eiki

AU - Luyten, Gré

AU - Inoue, Hiroko

AU - Saito, Ichiro

AU - Shimazaki, Jun

AU - Tsubota, Kazuo

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N2 - Purpose: To prospectively investigate the effects of acute passive cigarette smoke exposure on the ocular surface and tear film in healthy non-smokers. Methods: Twelve right eyes of 12 subjects without any ocular diseases were examined before, 5 min, and 24 h after 5 min of passive cigarette smoke exposure in a controlled smoke chamber. Tear samples were obtained before, 5 min and 24 h after smoke exposure to detect tear hexanoyl-lysine (HEL), acrolein and inflammatory cytokine concentrations. Tear evaporation rate, DR-1 tear film lipid layer interferometry, tear film break-up time (TBUT), ocular surface fluorescein staining (FS) and Rose Bengal staining (RB), Schirmer I test were performed before, 5 min, and 24 h after smoke exposure. Conjunctival impression cytology (IC) and brush cytology (BC) were carried out before and 24 h after smoke exposure. Results: Tear evaporation rate, tear lipid spread time, tear film break-up time, and vital staining scores showed significant worsening with passive smoke exposure. Tear HEL and IL-6 concentrations increased significantly 24 h after smoke exposure. Tear acrolein level showed an insignificant increase at 5 min. IC and RT-PCR revealed a significant reduction in goblet cell density, a shift toward higher squamous metaplasia grades and a significant downregulation of MUC5AC mRNA expression at 24 h. Conclusion: Even brief passive exposure to cigarette smoke in healthy non-smoker subjects was associated with adverse effects on the ocular surface health as evidenced by an increase of tear inflammatory cytokines, tear lipid peroxidation products and decrease of mucosal defense resulting in tear instability and damage to the ocular surface epithelia.

AB - Purpose: To prospectively investigate the effects of acute passive cigarette smoke exposure on the ocular surface and tear film in healthy non-smokers. Methods: Twelve right eyes of 12 subjects without any ocular diseases were examined before, 5 min, and 24 h after 5 min of passive cigarette smoke exposure in a controlled smoke chamber. Tear samples were obtained before, 5 min and 24 h after smoke exposure to detect tear hexanoyl-lysine (HEL), acrolein and inflammatory cytokine concentrations. Tear evaporation rate, DR-1 tear film lipid layer interferometry, tear film break-up time (TBUT), ocular surface fluorescein staining (FS) and Rose Bengal staining (RB), Schirmer I test were performed before, 5 min, and 24 h after smoke exposure. Conjunctival impression cytology (IC) and brush cytology (BC) were carried out before and 24 h after smoke exposure. Results: Tear evaporation rate, tear lipid spread time, tear film break-up time, and vital staining scores showed significant worsening with passive smoke exposure. Tear HEL and IL-6 concentrations increased significantly 24 h after smoke exposure. Tear acrolein level showed an insignificant increase at 5 min. IC and RT-PCR revealed a significant reduction in goblet cell density, a shift toward higher squamous metaplasia grades and a significant downregulation of MUC5AC mRNA expression at 24 h. Conclusion: Even brief passive exposure to cigarette smoke in healthy non-smoker subjects was associated with adverse effects on the ocular surface health as evidenced by an increase of tear inflammatory cytokines, tear lipid peroxidation products and decrease of mucosal defense resulting in tear instability and damage to the ocular surface epithelia.

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KW - Dry eye

KW - Hexanoyl-lysine

KW - Mucin

KW - Smoking

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