Telomere length of normal leukocytes is affected by a functional polymorphism of hTERT

Yumiko Matsubara, Mitsuru Murata, Tadashi Yoshida, Kiyoaki Watanabe, Ikuo Saito, Koichi Miyaki, Kazuyuki Omae, Yasuo Ikeda

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Abstract

Transcriptional regulation of human telomerase reverse transcriptase (hTERT), a catalytic subunit of telomerase, is essential for telomerase activity associated with telomere length. In this study, we investigated the effects of a -1327T/C polymorphism within the hTERT promoter region on the hTERT promoter activity and leukocyte telomere length in normal individuals. The promoter activity in the -1327T-sequence was significantly higher than that in the -1327C-sequence (p = 0.0004). For leukocyte telomere length, the -1327T-allele carriers had significantly longer than the -1327T-allele non-carriers (p = 0.0007). Also, there was no age-related shortening in leukocyte telomere length in the -1327T/T (p = 0.6633) and -1327T/C subjects (p = 0.1691), whereas there was clear age-related telomere shortening in the -1327C/C subjects (p = 0.0117). These findings suggest that the functional -1327T/C polymorphism of hTERT is associated with leukocyte telomere length in normal individuals.

Original languageEnglish
Pages (from-to)128-131
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume341
Issue number1
DOIs
Publication statusPublished - 2006 Mar 3

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Telomere
Polymorphism
Leukocytes
Telomerase
Alleles
Telomere Shortening
Genetic Promoter Regions
human TERT protein

Keywords

  • Human telomerase reverse transcriptase
  • Polymorphism
  • Telomere length

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Telomere length of normal leukocytes is affected by a functional polymorphism of hTERT. / Matsubara, Yumiko; Murata, Mitsuru; Yoshida, Tadashi; Watanabe, Kiyoaki; Saito, Ikuo; Miyaki, Koichi; Omae, Kazuyuki; Ikeda, Yasuo.

In: Biochemical and Biophysical Research Communications, Vol. 341, No. 1, 03.03.2006, p. 128-131.

Research output: Contribution to journalArticle

Matsubara, Yumiko ; Murata, Mitsuru ; Yoshida, Tadashi ; Watanabe, Kiyoaki ; Saito, Ikuo ; Miyaki, Koichi ; Omae, Kazuyuki ; Ikeda, Yasuo. / Telomere length of normal leukocytes is affected by a functional polymorphism of hTERT. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 341, No. 1. pp. 128-131.
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