Temperature- and pH-responsive aminopropyl-silica ion-exchange columns grafted with copolymers of N-isopropylacrylamide

Chikako Sakamoto, Yuji Okada, Hideko Kanazawa, Eri Ayano, Tetsuji Nishimura, Masanori Ando, Akihiko Kikuchi, Teruo Okano

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

We have designed copolymers of N-isopropylacrylamide, environmentally- responsive polymers, which respond to temperature and other external stimuli. In this study, we designed and synthesized copolymers that introduced ion-exchange groups. These copolymers responded to the temperature and the pH, and the copolymer-grafted aminopropyl silica beads were used as HPLC packing materials. This stationary phase altered the properties from hydrophilic to hydrophobic and from charge to non-charge by temperature and pH changes. We studied the separations of organic acids and phenylthiohydantoin-amino acids using environmentally-responsive chromatography, and confirmed the effects of the ion-exchange groups. The elution behaviors of these samples were controlled by the temperature changes without organic solvents in the mobile phase. It was confirmed that the interactions between the solute and stationary phase could be freely controlled by the temperature and the pH. Environmentally-responsive chromatography is expected to be applicable to the separation of pharmaceuticals and biomolecules, such as peptides, proteins and nucleic acids.

Original languageEnglish
Pages (from-to)247-253
Number of pages7
JournalJournal of Chromatography A
Volume1030
Issue number1-2
DOIs
Publication statusPublished - 2004 Mar 19

Fingerprint

Ion Exchange
Silicon Dioxide
Ion exchange
Copolymers
Temperature
Chromatography
Phenylthiohydantoin
Peptide Nucleic Acids
Organic acids
Biomolecules
Organic solvents
Nucleic Acids
Polymers
High Pressure Liquid Chromatography
N-isopropylacrylamide
Amino Acids
Peptides
Acids
Pharmaceutical Preparations
Proteins

Keywords

  • Amino acids
  • pH effects
  • Poly(isopropylacrylamide)
  • PTH derivatives
  • Stationary phases LC
  • Temperature effects

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Temperature- and pH-responsive aminopropyl-silica ion-exchange columns grafted with copolymers of N-isopropylacrylamide. / Sakamoto, Chikako; Okada, Yuji; Kanazawa, Hideko; Ayano, Eri; Nishimura, Tetsuji; Ando, Masanori; Kikuchi, Akihiko; Okano, Teruo.

In: Journal of Chromatography A, Vol. 1030, No. 1-2, 19.03.2004, p. 247-253.

Research output: Contribution to journalArticle

Sakamoto, Chikako ; Okada, Yuji ; Kanazawa, Hideko ; Ayano, Eri ; Nishimura, Tetsuji ; Ando, Masanori ; Kikuchi, Akihiko ; Okano, Teruo. / Temperature- and pH-responsive aminopropyl-silica ion-exchange columns grafted with copolymers of N-isopropylacrylamide. In: Journal of Chromatography A. 2004 ; Vol. 1030, No. 1-2. pp. 247-253.
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AU - Nishimura, Tetsuji

AU - Ando, Masanori

AU - Kikuchi, Akihiko

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AB - We have designed copolymers of N-isopropylacrylamide, environmentally- responsive polymers, which respond to temperature and other external stimuli. In this study, we designed and synthesized copolymers that introduced ion-exchange groups. These copolymers responded to the temperature and the pH, and the copolymer-grafted aminopropyl silica beads were used as HPLC packing materials. This stationary phase altered the properties from hydrophilic to hydrophobic and from charge to non-charge by temperature and pH changes. We studied the separations of organic acids and phenylthiohydantoin-amino acids using environmentally-responsive chromatography, and confirmed the effects of the ion-exchange groups. The elution behaviors of these samples were controlled by the temperature changes without organic solvents in the mobile phase. It was confirmed that the interactions between the solute and stationary phase could be freely controlled by the temperature and the pH. Environmentally-responsive chromatography is expected to be applicable to the separation of pharmaceuticals and biomolecules, such as peptides, proteins and nucleic acids.

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