TY - JOUR
T1 - Temperature-responsive chromatography for bioseparations
T2 - A review
AU - Nagase, Kenichi
AU - Kanazawa, Hideko
N1 - Funding Information:
This work was partly financially supported by a SENTAN grant ( JPMJSN16B3 ) from the Japan Science and Technology Agency (JST) and a Grant-in-Aid for Scientific Research (grant nos. 16H05083 and 19H02447 ) from the Japan Society for the Promotion of Science (JSPS) .
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/11/22
Y1 - 2020/11/22
N2 - In recent decades, in addition to existing small-molecule drug therapies, biomedical technology has also rapidly progressed, leading to the development of various therapies based on biopharmaceuticals and therapeutic cells. However, these materials require effective separation methods for their analysis and production. A representative separation method, which has been extensively studied, is the temperature-responsive chromatography system using poly(N-isopropylacrylamide) and its copolymers. Over the last 20 years, various temperature-responsive chromatographic techniques have been developed for the separation of different types of analytes by changing the copolymer composition, the polymer graft configuration, and the base materials of the stationary phase. The developed methods have been successfully applied for the separation of small-molecule drugs, peptides, and proteins, without affecting their biological activity, simply by changing the column temperature. Furthermore, temperature-modulated cell separation columns have been investigated for the separation of cells without changing their properties. Therefore, the developed methods can serve as effective tools for the current and future bioseparation of various biological compounds, biopharmaceutical proteins, and therapeutic cells that are currently used in therapies.
AB - In recent decades, in addition to existing small-molecule drug therapies, biomedical technology has also rapidly progressed, leading to the development of various therapies based on biopharmaceuticals and therapeutic cells. However, these materials require effective separation methods for their analysis and production. A representative separation method, which has been extensively studied, is the temperature-responsive chromatography system using poly(N-isopropylacrylamide) and its copolymers. Over the last 20 years, various temperature-responsive chromatographic techniques have been developed for the separation of different types of analytes by changing the copolymer composition, the polymer graft configuration, and the base materials of the stationary phase. The developed methods have been successfully applied for the separation of small-molecule drugs, peptides, and proteins, without affecting their biological activity, simply by changing the column temperature. Furthermore, temperature-modulated cell separation columns have been investigated for the separation of cells without changing their properties. Therefore, the developed methods can serve as effective tools for the current and future bioseparation of various biological compounds, biopharmaceutical proteins, and therapeutic cells that are currently used in therapies.
KW - Biopharmaceuticals
KW - Bioseparation
KW - Cell separation
KW - Temperature-responsive chromatography
KW - Thermoresponsive polymers
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U2 - 10.1016/j.aca.2020.07.075
DO - 10.1016/j.aca.2020.07.075
M3 - Review article
C2 - 33161981
AN - SCOPUS:85089859790
SN - 0003-2670
VL - 1138
SP - 191
EP - 212
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
ER -