Temperature-Responsive Chromatography Using Poly-(N-isopropylacrylamide) Hydrogel-Modified Silica

Hideko Kanazawa, Tatsuo Sunamoto, Eri Ayano, Yoshikazu Matsushima, Akihiko Kikuchi, Teruo Okano

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Poly(N-isopropylacrylamide) (PNIPAAm) has the sharpest phase transition of the class of thermo-sensitive N-alkyl acrylamide polymers. We developed a new method of HPLC using packing materials modified with cross-linked poly(N-isopropylacrylamide) (PNIPAAm) hydrogel. A temperature-responsive surface was prepared by polymerization of NIPAAm in the presence of a cross-linker on the silica support. The surface properties and functions of the stationary phases change in response to the external temperature. Therefore it easily changes the interaction of a solute with the surface with a constant aqueous mobile phase. A temperature-responsive elution behavior was observed on the separation of steroids and PTH-amino acids. The method is expected to be applicable to separation in the pharmaceutical and biomedical fields.

Original languageEnglish
Pages (from-to)45-48
Number of pages4
JournalAnalytical Sciences
Volume18
Issue number1
DOIs
Publication statusPublished - 2002

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Hydrogel
Chromatography
Silicon Dioxide
Acrylamide
Temperature
Surface properties
Polymers
Phase transitions
Steroids
Polymerization
Amino Acids
Pharmaceutical Preparations
poly-N-isopropylacrylamide

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Temperature-Responsive Chromatography Using Poly-(N-isopropylacrylamide) Hydrogel-Modified Silica. / Kanazawa, Hideko; Sunamoto, Tatsuo; Ayano, Eri; Matsushima, Yoshikazu; Kikuchi, Akihiko; Okano, Teruo.

In: Analytical Sciences, Vol. 18, No. 1, 2002, p. 45-48.

Research output: Contribution to journalArticle

Kanazawa, Hideko ; Sunamoto, Tatsuo ; Ayano, Eri ; Matsushima, Yoshikazu ; Kikuchi, Akihiko ; Okano, Teruo. / Temperature-Responsive Chromatography Using Poly-(N-isopropylacrylamide) Hydrogel-Modified Silica. In: Analytical Sciences. 2002 ; Vol. 18, No. 1. pp. 45-48.
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