Abstract
Poly(N-isopropylacrylamide) (PNIPAAm)-based temperature-responsive fluorescence polymer probes were developed using radical polymerization, with 3-mercaptopropionic acid as the chain-transfer agent, followed by activation of terminal carboxyl groups with N-hydroxysuccinimide and reaction with 5-aminofluorescein (FL). The lower critical solution temperatures (LCSTs) of the resulting fluorescent polymer probes differed depending on the copolymer composition, and had a sharp phase-transition (hydrophilic/hydrophobic) boundary at the LCST. The cellular uptakes of the fluorescent polymer probes were effectively suppressed below the LCST, and increased greatly above the LCST. In particular, the cellular uptake of a copolymer with N,N- dimethylaminopropylacrylamide, P(NIPAAm-co-DMAPAAm2%)-FL (LCST: 37.4 C), can be controlled within only 1 C near body temperature, which is suitable for biological applications. These results indicated that the cellular uptakes of thermoresponsive polymers could be accurately controlled by the temperature, and such polymers have potential applications in discriminating between normal and pathological cells, and in intracellular drug delivery systems with local hyperthermia.
| Original language | English |
|---|---|
| Pages (from-to) | 281-285 |
| Number of pages | 5 |
| Journal | ACS Macro Letters |
| Volume | 3 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2014 Mar 18 |
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ASJC Scopus subject areas
- Organic Chemistry
- Materials Chemistry
- Polymers and Plastics
- Inorganic Chemistry
Cite this
Temperature-responsive fluorescence polymer probes with accurate thermally controlled cellular uptakes. / Hiruta, Yuki; Shimamura, Mirai; Matsuura, Minami; Maekawa, Yutaro; Funatsu, Takaaki; Suzuki, Yuichi; Ayano, Eri; Okano, Teruo; Kanazawa, Hideko.
In: ACS Macro Letters, Vol. 3, No. 3, 18.03.2014, p. 281-285.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Temperature-responsive fluorescence polymer probes with accurate thermally controlled cellular uptakes
AU - Hiruta, Yuki
AU - Shimamura, Mirai
AU - Matsuura, Minami
AU - Maekawa, Yutaro
AU - Funatsu, Takaaki
AU - Suzuki, Yuichi
AU - Ayano, Eri
AU - Okano, Teruo
AU - Kanazawa, Hideko
PY - 2014/3/18
Y1 - 2014/3/18
N2 - Poly(N-isopropylacrylamide) (PNIPAAm)-based temperature-responsive fluorescence polymer probes were developed using radical polymerization, with 3-mercaptopropionic acid as the chain-transfer agent, followed by activation of terminal carboxyl groups with N-hydroxysuccinimide and reaction with 5-aminofluorescein (FL). The lower critical solution temperatures (LCSTs) of the resulting fluorescent polymer probes differed depending on the copolymer composition, and had a sharp phase-transition (hydrophilic/hydrophobic) boundary at the LCST. The cellular uptakes of the fluorescent polymer probes were effectively suppressed below the LCST, and increased greatly above the LCST. In particular, the cellular uptake of a copolymer with N,N- dimethylaminopropylacrylamide, P(NIPAAm-co-DMAPAAm2%)-FL (LCST: 37.4 C), can be controlled within only 1 C near body temperature, which is suitable for biological applications. These results indicated that the cellular uptakes of thermoresponsive polymers could be accurately controlled by the temperature, and such polymers have potential applications in discriminating between normal and pathological cells, and in intracellular drug delivery systems with local hyperthermia.
AB - Poly(N-isopropylacrylamide) (PNIPAAm)-based temperature-responsive fluorescence polymer probes were developed using radical polymerization, with 3-mercaptopropionic acid as the chain-transfer agent, followed by activation of terminal carboxyl groups with N-hydroxysuccinimide and reaction with 5-aminofluorescein (FL). The lower critical solution temperatures (LCSTs) of the resulting fluorescent polymer probes differed depending on the copolymer composition, and had a sharp phase-transition (hydrophilic/hydrophobic) boundary at the LCST. The cellular uptakes of the fluorescent polymer probes were effectively suppressed below the LCST, and increased greatly above the LCST. In particular, the cellular uptake of a copolymer with N,N- dimethylaminopropylacrylamide, P(NIPAAm-co-DMAPAAm2%)-FL (LCST: 37.4 C), can be controlled within only 1 C near body temperature, which is suitable for biological applications. These results indicated that the cellular uptakes of thermoresponsive polymers could be accurately controlled by the temperature, and such polymers have potential applications in discriminating between normal and pathological cells, and in intracellular drug delivery systems with local hyperthermia.
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U2 - 10.1021/mz5000569
DO - 10.1021/mz5000569
M3 - Article
AN - SCOPUS:84896446629
VL - 3
SP - 281
EP - 285
JO - ACS Macro Letters
JF - ACS Macro Letters
SN - 2161-1653
IS - 3
ER -