Temperature-responsive fluorescence polymer probes with accurate thermally controlled cellular uptakes

Yuki Hiruta; Mirai Shimamura; Minami Matsuura; Yutaro Maekawa; Takaaki Funatsu; Yuichi Suzuki; Eri Ayano; Teruo Okano; Hideko Kanazawa

doi:10.1021/mz5000569

Temperature-responsive fluorescence polymer probes with accurate thermally controlled cellular uptakes

Yuki Hiruta, Mirai Shimamura, Minami Matsuura, Yutaro Maekawa, Takaaki Funatsu, Yuichi Suzuki, Eri Ayano, Teruo Okano, Hideko Kanazawa

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46 Citations (Scopus)

Abstract

Poly(N-isopropylacrylamide) (PNIPAAm)-based temperature-responsive fluorescence polymer probes were developed using radical polymerization, with 3-mercaptopropionic acid as the chain-transfer agent, followed by activation of terminal carboxyl groups with N-hydroxysuccinimide and reaction with 5-aminofluorescein (FL). The lower critical solution temperatures (LCSTs) of the resulting fluorescent polymer probes differed depending on the copolymer composition, and had a sharp phase-transition (hydrophilic/hydrophobic) boundary at the LCST. The cellular uptakes of the fluorescent polymer probes were effectively suppressed below the LCST, and increased greatly above the LCST. In particular, the cellular uptake of a copolymer with N,N- dimethylaminopropylacrylamide, P(NIPAAm-co-DMAPAAm2%)-FL (LCST: 37.4 C), can be controlled within only 1 C near body temperature, which is suitable for biological applications. These results indicated that the cellular uptakes of thermoresponsive polymers could be accurately controlled by the temperature, and such polymers have potential applications in discriminating between normal and pathological cells, and in intracellular drug delivery systems with local hyperthermia.

Original language	English
Pages (from-to)	281-285
Number of pages	5
Journal	ACS Macro Letters
Volume	3
Issue number	3
DOIs	https://doi.org/10.1021/mz5000569
Publication status	Published - 2014 Mar 18

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ASJC Scopus subject areas

Organic Chemistry
Polymers and Plastics
Inorganic Chemistry
Materials Chemistry

Cite this

Hiruta, Y., Shimamura, M., Matsuura, M., Maekawa, Y., Funatsu, T., Suzuki, Y., Ayano, E., Okano, T., & Kanazawa, H. (2014). Temperature-responsive fluorescence polymer probes with accurate thermally controlled cellular uptakes. ACS Macro Letters, 3(3), 281-285. https://doi.org/10.1021/mz5000569

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abstract = "Poly(N-isopropylacrylamide) (PNIPAAm)-based temperature-responsive fluorescence polymer probes were developed using radical polymerization, with 3-mercaptopropionic acid as the chain-transfer agent, followed by activation of terminal carboxyl groups with N-hydroxysuccinimide and reaction with 5-aminofluorescein (FL). The lower critical solution temperatures (LCSTs) of the resulting fluorescent polymer probes differed depending on the copolymer composition, and had a sharp phase-transition (hydrophilic/hydrophobic) boundary at the LCST. The cellular uptakes of the fluorescent polymer probes were effectively suppressed below the LCST, and increased greatly above the LCST. In particular, the cellular uptake of a copolymer with N,N- dimethylaminopropylacrylamide, P(NIPAAm-co-DMAPAAm2%)-FL (LCST: 37.4 C), can be controlled within only 1 C near body temperature, which is suitable for biological applications. These results indicated that the cellular uptakes of thermoresponsive polymers could be accurately controlled by the temperature, and such polymers have potential applications in discriminating between normal and pathological cells, and in intracellular drug delivery systems with local hyperthermia.",

author = "Yuki Hiruta and Mirai Shimamura and Minami Matsuura and Yutaro Maekawa and Takaaki Funatsu and Yuichi Suzuki and Eri Ayano and Teruo Okano and Hideko Kanazawa",

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AU - Hiruta, Yuki

AU - Shimamura, Mirai

AU - Matsuura, Minami

AU - Maekawa, Yutaro

AU - Funatsu, Takaaki

AU - Suzuki, Yuichi

AU - Ayano, Eri

AU - Okano, Teruo

AU - Kanazawa, Hideko

PY - 2014/3/18

Y1 - 2014/3/18

N2 - Poly(N-isopropylacrylamide) (PNIPAAm)-based temperature-responsive fluorescence polymer probes were developed using radical polymerization, with 3-mercaptopropionic acid as the chain-transfer agent, followed by activation of terminal carboxyl groups with N-hydroxysuccinimide and reaction with 5-aminofluorescein (FL). The lower critical solution temperatures (LCSTs) of the resulting fluorescent polymer probes differed depending on the copolymer composition, and had a sharp phase-transition (hydrophilic/hydrophobic) boundary at the LCST. The cellular uptakes of the fluorescent polymer probes were effectively suppressed below the LCST, and increased greatly above the LCST. In particular, the cellular uptake of a copolymer with N,N- dimethylaminopropylacrylamide, P(NIPAAm-co-DMAPAAm2%)-FL (LCST: 37.4 C), can be controlled within only 1 C near body temperature, which is suitable for biological applications. These results indicated that the cellular uptakes of thermoresponsive polymers could be accurately controlled by the temperature, and such polymers have potential applications in discriminating between normal and pathological cells, and in intracellular drug delivery systems with local hyperthermia.

AB - Poly(N-isopropylacrylamide) (PNIPAAm)-based temperature-responsive fluorescence polymer probes were developed using radical polymerization, with 3-mercaptopropionic acid as the chain-transfer agent, followed by activation of terminal carboxyl groups with N-hydroxysuccinimide and reaction with 5-aminofluorescein (FL). The lower critical solution temperatures (LCSTs) of the resulting fluorescent polymer probes differed depending on the copolymer composition, and had a sharp phase-transition (hydrophilic/hydrophobic) boundary at the LCST. The cellular uptakes of the fluorescent polymer probes were effectively suppressed below the LCST, and increased greatly above the LCST. In particular, the cellular uptake of a copolymer with N,N- dimethylaminopropylacrylamide, P(NIPAAm-co-DMAPAAm2%)-FL (LCST: 37.4 C), can be controlled within only 1 C near body temperature, which is suitable for biological applications. These results indicated that the cellular uptakes of thermoresponsive polymers could be accurately controlled by the temperature, and such polymers have potential applications in discriminating between normal and pathological cells, and in intracellular drug delivery systems with local hyperthermia.

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10.1021/mz5000569