Temperature-responsive intelligent interfaces for biomolecular separation and cell sheet engineering

Kenichi Nagase, Jun Kobayashi, Teruo Okano

Research output: Contribution to journalReview articlepeer-review

219 Citations (Scopus)


Temperature-responsive intelligent surfaces, prepared by the modification of an interface with poly(N-isopropylacrylamide) and its derivatives, have been used for biomedical applications. Such surfaces exhibit temperature-responsive hydrophilic/hydrophobic alterations with external temperature changes, which, in turn, result in thermally modulated interactions with biomolecules and cells. In this review, we focus on the application of these intelligent surfaces to chromatographic separation and cell cultures. Chromatographic separations using several types of intelligent surfaces are mentioned briefly, and various effects related to the separation of bioactive compounds are discussed, including wettability, copolymer composition and graft polymer architecture. Similarly, we also summarize temperature-responsive cell culture substrates that allow the recovery of confluent cell monolayers as contiguous living cell sheets for tissue-engineering applications. The key factors in temperature-dependent cell adhesion/detachment control are discussed from the viewpoint of grafting temperature-responsive polymers, and new methodologies for effective cell sheet culturing and the construction of thick tissues are summarized.

Original languageEnglish
Pages (from-to)S293-S309
JournalJournal of the Royal Society Interface
Issue numberSUPPL. 3
Publication statusPublished - 2009 Jun 6
Externally publishedYes


  • Cell sheet engineering
  • Intelligent materials
  • Living radical polymerization
  • Poly(N- isopropylacrylamide)
  • Temperature-responsive polymer
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering


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