Temperature-responsive stationary phase utilizing a polymer of proline derivative for hydrophobic interaction chromatography using an aqueous mobile phase

Hideko Kanazawa, Eri Ayano, Chikako Sakamoto, Reiko Yoda, Akihiko Kikuchi, Teruo Okano

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A new method of chromatography is proposed, utilizing a thermo-responsive polymer carrying an amino acid ester residue for the stationary phase of high-performance liquid chromatography (HPLC). We have been investigating the new concept of chromatography, a temperature-responsive chromatography, using temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm)-modified surface for HPLC with a constant aqueous media as the mobile phase. In this study, we designed and synthesized thermo-responsive poly(acryloyl-l-proline methyl ester) and its copolymer with N-isopropylacrylamide (NIPAAm). Homopolymers of acryloyl-l-proline methyl ester and copolymer were prepared by the reaction of radical telomerization. These polymers underwent a reversible phase transition from water-soluble forms into aggregates by changing the temperature, similar to PNIPAAm. The surface properties and functions of stationary phases modified with poly(acryloyl-l-proline methyl ester) were controlled by the external temperature. In the chromatographic system, we separated steroids and amino acids with a variety of hydrophobicities using a sole aqueous mobile phase. In contrast to a PNIPAAm-modified surface, a poly(acryloyl-l-proline methyl ester)-modified surface showed a greater affinity for hydrophobic amino acids.

Original languageEnglish
Pages (from-to)152-158
Number of pages7
JournalJournal of Chromatography A
Volume1106
Issue number1-2
DOIs
Publication statusPublished - 2006 Feb 17

Fingerprint

Chromatography
Hydrophobic and Hydrophilic Interactions
Proline
Polymers
Esters
Derivatives
Temperature
High performance liquid chromatography
Amino Acids
Copolymers
High Pressure Liquid Chromatography
Surface Properties
Phase Transition
Hydrophobicity
Homopolymerization
Surface properties
Phase transitions
Steroids
Water
poly-N-isopropylacrylamide

Keywords

  • Amino acids
  • HPLC
  • Hydrophobic interaction chromatography
  • Poly(acryloyl-l-proline methyl ester)
  • Poly(N-isopropylacrylamide)
  • Separation
  • Steroids
  • Temperature-responsive polymer

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Temperature-responsive stationary phase utilizing a polymer of proline derivative for hydrophobic interaction chromatography using an aqueous mobile phase. / Kanazawa, Hideko; Ayano, Eri; Sakamoto, Chikako; Yoda, Reiko; Kikuchi, Akihiko; Okano, Teruo.

In: Journal of Chromatography A, Vol. 1106, No. 1-2, 17.02.2006, p. 152-158.

Research output: Contribution to journalArticle

Kanazawa, Hideko ; Ayano, Eri ; Sakamoto, Chikako ; Yoda, Reiko ; Kikuchi, Akihiko ; Okano, Teruo. / Temperature-responsive stationary phase utilizing a polymer of proline derivative for hydrophobic interaction chromatography using an aqueous mobile phase. In: Journal of Chromatography A. 2006 ; Vol. 1106, No. 1-2. pp. 152-158.
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