Tempo-spatial patterns of bacterial community composition in the western North Pacific Ocean

Takafumi Kataoka, Yoshikuni Hodoki, Koji Suzuki, Hiroaki Saito, Seigo Higashi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In the western North Pacific, where subarctic Oyashio waters encounter subtropical Kuroshio waters, phylotype composition of heterotrophic bacteria was estimated by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified bacterial 16S ribosomal DNA. Total bacterial abundance was also measured by flow cytometry. The study area was divided into four water masses: coastal, Oyashio, Kuroshio, and the Kuroshio-Oyashio transition. Abundances of heterotrophic bacteria in the Oyashio, Kuroshio, and Kuroshio-Oyashio transition regions ranged from 0.2 × 105 cells mL- 1 to 1.4 × 105 cells mL- 1 and were not significantly different, except in the Oyashio region during September. Bacterial compositions were distinct in each water mass. Furthermore, phylotype distributions differed between surface and subsurface waters in the Kuroshio-Oyashio transition region. Out of 61 DGGE bands obtained, 41 were successfully identified as 31 phylotypes: 22 Gammaproteobacteria, 4 Alphaproteobacteria, 2 unknown bacteria, 2 cyanobacteria, and 1 plastid. Although the Gammaproteobacteria OM60 clade was eurytopic in the study area, Psychrobacter glacincola and the uncultured Gammaproteobacteria SAR92 clade were often observed in the Oyashio region. Overall, our results indicated that Gammaproteobacteria were predominant in the bacterial community, which was influenced by the hydrographic properties of each water mass in the study area.

Original languageEnglish
Pages (from-to)197-207
Number of pages11
JournalJournal of Marine Systems
Volume77
Issue number1-2
DOIs
Publication statusPublished - 2009 Apr
Externally publishedYes

Fingerprint

gamma-Proteobacteria
bacterial communities
Pacific Ocean
community composition
water mass
denaturing gradient gel electrophoresis
Psychrobacter glacincola
bacterium
electrokinesis
bacteria
gel
water
alpha-Proteobacteria
plastid
flow cytometry
ribosomal DNA
plastids
Cyanobacteria
cyanobacterium
DNA

Keywords

  • DGGE
  • Kuroshio
  • Marine bacteria
  • Oyashio
  • Pacific Ocean
  • Phylotype

ASJC Scopus subject areas

  • Aquatic Science
  • Oceanography
  • Ecology, Evolution, Behavior and Systematics

Cite this

Tempo-spatial patterns of bacterial community composition in the western North Pacific Ocean. / Kataoka, Takafumi; Hodoki, Yoshikuni; Suzuki, Koji; Saito, Hiroaki; Higashi, Seigo.

In: Journal of Marine Systems, Vol. 77, No. 1-2, 04.2009, p. 197-207.

Research output: Contribution to journalArticle

Kataoka, Takafumi ; Hodoki, Yoshikuni ; Suzuki, Koji ; Saito, Hiroaki ; Higashi, Seigo. / Tempo-spatial patterns of bacterial community composition in the western North Pacific Ocean. In: Journal of Marine Systems. 2009 ; Vol. 77, No. 1-2. pp. 197-207.
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