Temporal alterations of endothelial-vasodilator functions in lung injury induced by monocrotaline

Kazuhiro Yamaguchi, Yae Kanai, Koichiro Asano, Tomoaki Takasugi, Tsuyoshi Tanaka, Megumi Yasuoka, Yasuhiro Hosoda

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

To assess the time course of alterations in pulmonary endothelial vasodilator functions during pathological development of pulmonary hypertension (PH) and right ventricular hypertrophy (RVH), we examined changes in serotonin (5-HT) removal rates and the production of prostacyclin (PGI2) and nitric oxide (NO) in isolated rat lungs harvested at various times after single exposure to monocrotaline (MCT). We assessed the generation of vasodilator substances under conditions of both the absence and the presence of 5-HT in lungs perfused with blood-free solution. Major findings included: (i) remodeling of the pulmonary vasculature associated with RVH evident 14 days after MCT injection; (ii) the capacity for 5-HT removal was suppressed at day 1 and 7 but had been restored by day 14 after MCT exposure; (iii) basal PGI2 production in the absence of 5-HT was augmented at day 1 but had returned to control levels in lungs harvested 7 or 14 days postinjection of MCT; (iv) PGI2 production evoked by 5-HT was suppressed in MCT lungs obtained at all time points examined; (v) basal NO production was suppressed at day 1 but enhanced at day 7 and 14 in MCT lungs; (vi) NO production elicited by 5-HT stimulation in 1-day-MCT lungs was obviously suppressed while that in 7- and 14-day-MCT lungs had been restored to the control level. These findings suggest that transitional changes in endothelial functions including 5-HT removal and production of vasodilators in MCT lungs do not follow the same time course.

Original languageEnglish
Pages (from-to)47-58
Number of pages12
JournalRespiration Physiology
Volume107
Issue number1
DOIs
Publication statusPublished - 1997 Jan

Fingerprint

Monocrotaline
Lung Injury
Vasodilator Agents
Serotonin
Lung
Epoprostenol
Right Ventricular Hypertrophy
Nitric Oxide
Pulmonary Hypertension

Keywords

  • hypertension
  • mammals
  • mediators
  • monocrataline
  • NO
  • PGI
  • pharmacological agents
  • pulmonary circulation
  • rat
  • serotonin

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

Temporal alterations of endothelial-vasodilator functions in lung injury induced by monocrotaline. / Yamaguchi, Kazuhiro; Kanai, Yae; Asano, Koichiro; Takasugi, Tomoaki; Tanaka, Tsuyoshi; Yasuoka, Megumi; Hosoda, Yasuhiro.

In: Respiration Physiology, Vol. 107, No. 1, 01.1997, p. 47-58.

Research output: Contribution to journalArticle

Yamaguchi, Kazuhiro ; Kanai, Yae ; Asano, Koichiro ; Takasugi, Tomoaki ; Tanaka, Tsuyoshi ; Yasuoka, Megumi ; Hosoda, Yasuhiro. / Temporal alterations of endothelial-vasodilator functions in lung injury induced by monocrotaline. In: Respiration Physiology. 1997 ; Vol. 107, No. 1. pp. 47-58.
@article{fbfc0ee3abaa40d38cb8ce09c09c73e5,
title = "Temporal alterations of endothelial-vasodilator functions in lung injury induced by monocrotaline",
abstract = "To assess the time course of alterations in pulmonary endothelial vasodilator functions during pathological development of pulmonary hypertension (PH) and right ventricular hypertrophy (RVH), we examined changes in serotonin (5-HT) removal rates and the production of prostacyclin (PGI2) and nitric oxide (NO) in isolated rat lungs harvested at various times after single exposure to monocrotaline (MCT). We assessed the generation of vasodilator substances under conditions of both the absence and the presence of 5-HT in lungs perfused with blood-free solution. Major findings included: (i) remodeling of the pulmonary vasculature associated with RVH evident 14 days after MCT injection; (ii) the capacity for 5-HT removal was suppressed at day 1 and 7 but had been restored by day 14 after MCT exposure; (iii) basal PGI2 production in the absence of 5-HT was augmented at day 1 but had returned to control levels in lungs harvested 7 or 14 days postinjection of MCT; (iv) PGI2 production evoked by 5-HT was suppressed in MCT lungs obtained at all time points examined; (v) basal NO production was suppressed at day 1 but enhanced at day 7 and 14 in MCT lungs; (vi) NO production elicited by 5-HT stimulation in 1-day-MCT lungs was obviously suppressed while that in 7- and 14-day-MCT lungs had been restored to the control level. These findings suggest that transitional changes in endothelial functions including 5-HT removal and production of vasodilators in MCT lungs do not follow the same time course.",
keywords = "hypertension, mammals, mediators, monocrataline, NO, PGI, pharmacological agents, pulmonary circulation, rat, serotonin",
author = "Kazuhiro Yamaguchi and Yae Kanai and Koichiro Asano and Tomoaki Takasugi and Tsuyoshi Tanaka and Megumi Yasuoka and Yasuhiro Hosoda",
year = "1997",
month = "1",
doi = "10.1016/S0034-5687(96)02501-7",
language = "English",
volume = "107",
pages = "47--58",
journal = "Respiratory Physiology and Neurobiology",
issn = "1569-9048",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Temporal alterations of endothelial-vasodilator functions in lung injury induced by monocrotaline

AU - Yamaguchi, Kazuhiro

AU - Kanai, Yae

AU - Asano, Koichiro

AU - Takasugi, Tomoaki

AU - Tanaka, Tsuyoshi

AU - Yasuoka, Megumi

AU - Hosoda, Yasuhiro

PY - 1997/1

Y1 - 1997/1

N2 - To assess the time course of alterations in pulmonary endothelial vasodilator functions during pathological development of pulmonary hypertension (PH) and right ventricular hypertrophy (RVH), we examined changes in serotonin (5-HT) removal rates and the production of prostacyclin (PGI2) and nitric oxide (NO) in isolated rat lungs harvested at various times after single exposure to monocrotaline (MCT). We assessed the generation of vasodilator substances under conditions of both the absence and the presence of 5-HT in lungs perfused with blood-free solution. Major findings included: (i) remodeling of the pulmonary vasculature associated with RVH evident 14 days after MCT injection; (ii) the capacity for 5-HT removal was suppressed at day 1 and 7 but had been restored by day 14 after MCT exposure; (iii) basal PGI2 production in the absence of 5-HT was augmented at day 1 but had returned to control levels in lungs harvested 7 or 14 days postinjection of MCT; (iv) PGI2 production evoked by 5-HT was suppressed in MCT lungs obtained at all time points examined; (v) basal NO production was suppressed at day 1 but enhanced at day 7 and 14 in MCT lungs; (vi) NO production elicited by 5-HT stimulation in 1-day-MCT lungs was obviously suppressed while that in 7- and 14-day-MCT lungs had been restored to the control level. These findings suggest that transitional changes in endothelial functions including 5-HT removal and production of vasodilators in MCT lungs do not follow the same time course.

AB - To assess the time course of alterations in pulmonary endothelial vasodilator functions during pathological development of pulmonary hypertension (PH) and right ventricular hypertrophy (RVH), we examined changes in serotonin (5-HT) removal rates and the production of prostacyclin (PGI2) and nitric oxide (NO) in isolated rat lungs harvested at various times after single exposure to monocrotaline (MCT). We assessed the generation of vasodilator substances under conditions of both the absence and the presence of 5-HT in lungs perfused with blood-free solution. Major findings included: (i) remodeling of the pulmonary vasculature associated with RVH evident 14 days after MCT injection; (ii) the capacity for 5-HT removal was suppressed at day 1 and 7 but had been restored by day 14 after MCT exposure; (iii) basal PGI2 production in the absence of 5-HT was augmented at day 1 but had returned to control levels in lungs harvested 7 or 14 days postinjection of MCT; (iv) PGI2 production evoked by 5-HT was suppressed in MCT lungs obtained at all time points examined; (v) basal NO production was suppressed at day 1 but enhanced at day 7 and 14 in MCT lungs; (vi) NO production elicited by 5-HT stimulation in 1-day-MCT lungs was obviously suppressed while that in 7- and 14-day-MCT lungs had been restored to the control level. These findings suggest that transitional changes in endothelial functions including 5-HT removal and production of vasodilators in MCT lungs do not follow the same time course.

KW - hypertension

KW - mammals

KW - mediators

KW - monocrataline

KW - NO

KW - PGI

KW - pharmacological agents

KW - pulmonary circulation

KW - rat

KW - serotonin

UR - http://www.scopus.com/inward/record.url?scp=0031002959&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031002959&partnerID=8YFLogxK

U2 - 10.1016/S0034-5687(96)02501-7

DO - 10.1016/S0034-5687(96)02501-7

M3 - Article

C2 - 9089893

AN - SCOPUS:0031002959

VL - 107

SP - 47

EP - 58

JO - Respiratory Physiology and Neurobiology

JF - Respiratory Physiology and Neurobiology

SN - 1569-9048

IS - 1

ER -