Temporal profile and cellular localization of interleukin-6 protein after focal cerebral ischemia in rats

Shigeaki Suzuki, Kortaro Tanaka, Shigeru Nogawa, Eiichiro Nagata, Daisuke Ito, Tomohisa Dembo, Yasuo Fukuuchi

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Although interleukin-6 (IL-6) has various neuroprotective effects against cerebral ischemia, the topographic distribution and cellular source of IL-6 after cerebral ischemia remain unclear. In the current study, the localization of IL-6 protein was immunohistochemically examined in rats after 3.5, 12, 24, and 48 hours of reperfusion after 1.5 hours of middle cerebral artery occlusion. Middle cerebral artery occlusion was induced by the intraluminal suture method. The specificity of the anti-IL-6 antibody used in the current study was confirmed by Western blot analysis and an immunoabsorption text. To identify tile cellular source, lectin histochemical study and immunohistochemical study with microtubule-associated protein-2, ED1 and glial fibrillary acidic protein also were carried out. The sham group did not show any clear IL-6 immunoreactivity. After 3.5 hours of reperfusion, IL-6 immunoreactivity was first detected on tile reperfused side, and it was upregulated, especially in the periinfarct region, after 24 hours of reperfusion. Also, IL-6 was expressed after 3.5 hours of reperfusion in the contralateral cerebral cortex and bilateral hippocampi. Double staining showed that the cells containing IL-6 were neurons and round-type microglia, not astrocytes. The current findings suggest that IL-6 expression in ischemically threatened neurons and reactive microglia is closely associated with brain tissue neuroprotective mechanisms against cerebral ischemia.

Original languageEnglish
Pages (from-to)1256-1262
Number of pages7
JournalJournal of Cerebral Blood Flow and Metabolism
Volume19
Issue number11
Publication statusPublished - 1999

Fingerprint

Brain Ischemia
Interleukin-6
Proteins
Reperfusion
Middle Cerebral Artery Infarction
Microglia
Neurons
Microtubule-Associated Proteins
Glial Fibrillary Acidic Protein
Neuroprotective Agents
Lectins
Astrocytes
Cerebral Cortex
Sutures
Hippocampus
Western Blotting
Staining and Labeling
Antibodies
Brain

Keywords

  • Focal ischemia
  • Immunohistochemistry
  • Interleukin-6
  • Microglia
  • Neuron
  • Rats

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Temporal profile and cellular localization of interleukin-6 protein after focal cerebral ischemia in rats. / Suzuki, Shigeaki; Tanaka, Kortaro; Nogawa, Shigeru; Nagata, Eiichiro; Ito, Daisuke; Dembo, Tomohisa; Fukuuchi, Yasuo.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 19, No. 11, 1999, p. 1256-1262.

Research output: Contribution to journalArticle

Suzuki, Shigeaki ; Tanaka, Kortaro ; Nogawa, Shigeru ; Nagata, Eiichiro ; Ito, Daisuke ; Dembo, Tomohisa ; Fukuuchi, Yasuo. / Temporal profile and cellular localization of interleukin-6 protein after focal cerebral ischemia in rats. In: Journal of Cerebral Blood Flow and Metabolism. 1999 ; Vol. 19, No. 11. pp. 1256-1262.
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