Terminal differentiation of murine resident peritoneal macrophages is characterized by expression of the STK protein tyrosine kinase, a receptor for macrophage-stimulating protein

A. Iwama, M. H. Wang, N. Yamaguchi, N. Ohno, K. Okano, T. Sudo, M. Takeya, F. Gervais, C. Morissette, E. J. Leonard, T. Suda

Research output: Contribution to journalArticle

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Abstract

STK, a new member of the hepatocyte growth factor receptor family, is the receptor for macrophage-stimulating protein (MSP), which acts on murine resident peritoneal macrophages. We established polyclonal and monoclonal antibodies against STK and characterized the structure of STK protein and STK expression on cells of the mononuclear phagocyte system. Western blotting showed that the STK transcript is translated into a single-chain precursor and then cleaved into a 165-kD disulfide-linked heterodimer composed of a 35- kD α-chain and a 144-kD β-chain. Western blotting detected STK protein on resident peritoneal macrophages, a target of MSP, and showed that it was autophosphorylated in cells stimulated by MSP. By flow cytometric analysis using a monoclonal anti-STK antibody, we showed that STK protein is expressed on restricted macrophage populations such as resident peritoneal macrophages, but not on exudate peritoneal macrophages or mononuclear phagocytes of the bone marrow, peripheral blood, spleen, or alveoli. Resident peritoneal macrophages were classified into two fractions according to their reactivity with an anti-STK antibody and a marker antibody for macrophages: STK(high)- F4/80(high) cells and STK(negative)-F4/80(low) cells. Acute exudative macrophages were all STK(negative)-F4/80(low), but they gradually became predominantly STK(high)-F4/80(low) several days after entrance into the peritoneal cavity. These results showed that after monocytes migrate into the peritoneal cavity, they undergo terminal differentiation in the peritoneal microenvironment. This is the first evidence of tissue-specific terminal differentiation of peritoneal macrophages, and this terminal differentiation can be characterized by the expression of STK receptor tyrosine kinase.

Original languageEnglish
Pages (from-to)3394-3403
Number of pages10
JournalBlood
Volume86
Issue number9
Publication statusPublished - 1995

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Macrophages
Peritoneal Macrophages
Receptor Protein-Tyrosine Kinases
Peritoneal Cavity
Anti-Idiotypic Antibodies
Western Blotting
Monoclonal Antibodies
Proto-Oncogene Proteins c-met
Mononuclear Phagocyte System
Proteins
Antibodies
Exudates and Transudates
Phagocytes
Disulfides
macrophage stimulating protein
RON protein
Monocytes
Spleen
Bone Marrow
Bone

ASJC Scopus subject areas

  • Hematology

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Terminal differentiation of murine resident peritoneal macrophages is characterized by expression of the STK protein tyrosine kinase, a receptor for macrophage-stimulating protein. / Iwama, A.; Wang, M. H.; Yamaguchi, N.; Ohno, N.; Okano, K.; Sudo, T.; Takeya, M.; Gervais, F.; Morissette, C.; Leonard, E. J.; Suda, T.

In: Blood, Vol. 86, No. 9, 1995, p. 3394-3403.

Research output: Contribution to journalArticle

Iwama, A, Wang, MH, Yamaguchi, N, Ohno, N, Okano, K, Sudo, T, Takeya, M, Gervais, F, Morissette, C, Leonard, EJ & Suda, T 1995, 'Terminal differentiation of murine resident peritoneal macrophages is characterized by expression of the STK protein tyrosine kinase, a receptor for macrophage-stimulating protein', Blood, vol. 86, no. 9, pp. 3394-3403.
Iwama, A. ; Wang, M. H. ; Yamaguchi, N. ; Ohno, N. ; Okano, K. ; Sudo, T. ; Takeya, M. ; Gervais, F. ; Morissette, C. ; Leonard, E. J. ; Suda, T. / Terminal differentiation of murine resident peritoneal macrophages is characterized by expression of the STK protein tyrosine kinase, a receptor for macrophage-stimulating protein. In: Blood. 1995 ; Vol. 86, No. 9. pp. 3394-3403.
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abstract = "STK, a new member of the hepatocyte growth factor receptor family, is the receptor for macrophage-stimulating protein (MSP), which acts on murine resident peritoneal macrophages. We established polyclonal and monoclonal antibodies against STK and characterized the structure of STK protein and STK expression on cells of the mononuclear phagocyte system. Western blotting showed that the STK transcript is translated into a single-chain precursor and then cleaved into a 165-kD disulfide-linked heterodimer composed of a 35- kD α-chain and a 144-kD β-chain. Western blotting detected STK protein on resident peritoneal macrophages, a target of MSP, and showed that it was autophosphorylated in cells stimulated by MSP. By flow cytometric analysis using a monoclonal anti-STK antibody, we showed that STK protein is expressed on restricted macrophage populations such as resident peritoneal macrophages, but not on exudate peritoneal macrophages or mononuclear phagocytes of the bone marrow, peripheral blood, spleen, or alveoli. Resident peritoneal macrophages were classified into two fractions according to their reactivity with an anti-STK antibody and a marker antibody for macrophages: STK(high)- F4/80(high) cells and STK(negative)-F4/80(low) cells. Acute exudative macrophages were all STK(negative)-F4/80(low), but they gradually became predominantly STK(high)-F4/80(low) several days after entrance into the peritoneal cavity. These results showed that after monocytes migrate into the peritoneal cavity, they undergo terminal differentiation in the peritoneal microenvironment. This is the first evidence of tissue-specific terminal differentiation of peritoneal macrophages, and this terminal differentiation can be characterized by the expression of STK receptor tyrosine kinase.",
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T1 - Terminal differentiation of murine resident peritoneal macrophages is characterized by expression of the STK protein tyrosine kinase, a receptor for macrophage-stimulating protein

AU - Iwama, A.

AU - Wang, M. H.

AU - Yamaguchi, N.

AU - Ohno, N.

AU - Okano, K.

AU - Sudo, T.

AU - Takeya, M.

AU - Gervais, F.

AU - Morissette, C.

AU - Leonard, E. J.

AU - Suda, T.

PY - 1995

Y1 - 1995

N2 - STK, a new member of the hepatocyte growth factor receptor family, is the receptor for macrophage-stimulating protein (MSP), which acts on murine resident peritoneal macrophages. We established polyclonal and monoclonal antibodies against STK and characterized the structure of STK protein and STK expression on cells of the mononuclear phagocyte system. Western blotting showed that the STK transcript is translated into a single-chain precursor and then cleaved into a 165-kD disulfide-linked heterodimer composed of a 35- kD α-chain and a 144-kD β-chain. Western blotting detected STK protein on resident peritoneal macrophages, a target of MSP, and showed that it was autophosphorylated in cells stimulated by MSP. By flow cytometric analysis using a monoclonal anti-STK antibody, we showed that STK protein is expressed on restricted macrophage populations such as resident peritoneal macrophages, but not on exudate peritoneal macrophages or mononuclear phagocytes of the bone marrow, peripheral blood, spleen, or alveoli. Resident peritoneal macrophages were classified into two fractions according to their reactivity with an anti-STK antibody and a marker antibody for macrophages: STK(high)- F4/80(high) cells and STK(negative)-F4/80(low) cells. Acute exudative macrophages were all STK(negative)-F4/80(low), but they gradually became predominantly STK(high)-F4/80(low) several days after entrance into the peritoneal cavity. These results showed that after monocytes migrate into the peritoneal cavity, they undergo terminal differentiation in the peritoneal microenvironment. This is the first evidence of tissue-specific terminal differentiation of peritoneal macrophages, and this terminal differentiation can be characterized by the expression of STK receptor tyrosine kinase.

AB - STK, a new member of the hepatocyte growth factor receptor family, is the receptor for macrophage-stimulating protein (MSP), which acts on murine resident peritoneal macrophages. We established polyclonal and monoclonal antibodies against STK and characterized the structure of STK protein and STK expression on cells of the mononuclear phagocyte system. Western blotting showed that the STK transcript is translated into a single-chain precursor and then cleaved into a 165-kD disulfide-linked heterodimer composed of a 35- kD α-chain and a 144-kD β-chain. Western blotting detected STK protein on resident peritoneal macrophages, a target of MSP, and showed that it was autophosphorylated in cells stimulated by MSP. By flow cytometric analysis using a monoclonal anti-STK antibody, we showed that STK protein is expressed on restricted macrophage populations such as resident peritoneal macrophages, but not on exudate peritoneal macrophages or mononuclear phagocytes of the bone marrow, peripheral blood, spleen, or alveoli. Resident peritoneal macrophages were classified into two fractions according to their reactivity with an anti-STK antibody and a marker antibody for macrophages: STK(high)- F4/80(high) cells and STK(negative)-F4/80(low) cells. Acute exudative macrophages were all STK(negative)-F4/80(low), but they gradually became predominantly STK(high)-F4/80(low) several days after entrance into the peritoneal cavity. These results showed that after monocytes migrate into the peritoneal cavity, they undergo terminal differentiation in the peritoneal microenvironment. This is the first evidence of tissue-specific terminal differentiation of peritoneal macrophages, and this terminal differentiation can be characterized by the expression of STK receptor tyrosine kinase.

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