TY - JOUR
T1 - Th17 Cell Induction by Adhesion of Microbes to Intestinal Epithelial Cells
AU - Atarashi, Koji
AU - Tanoue, Takeshi
AU - Ando, Minoru
AU - Kamada, Nobuhiko
AU - Nagano, Yuji
AU - Narushima, Seiko
AU - Suda, Wataru
AU - Imaoka, Akemi
AU - Setoyama, Hiromi
AU - Nagamori, Takashi
AU - Ishikawa, Eiji
AU - Shima, Tatsuichiro
AU - Hara, Taeko
AU - Kado, Shoichi
AU - Jinnohara, Toshi
AU - Ohno, Hiroshi
AU - Kondo, Takashi
AU - Toyooka, Kiminori
AU - Watanabe, Eiichiro
AU - Yokoyama, Shin Ichiro
AU - Tokoro, Shunji
AU - Mori, Hiroshi
AU - Noguchi, Yurika
AU - Morita, Hidetoshi
AU - Ivanov, Ivaylo I.
AU - Sugiyama, Tsuyoshi
AU - Nuñez, Gabriel
AU - Camp, J. Gray
AU - Hattori, Masahira
AU - Umesaki, Yoshinori
AU - Honda, Kenya
N1 - Funding Information:
This work was supported by the Japan Society for the Promotion of Science NEXT program, Health Labor Sciences Research Grant, the Waksman Foundation of Japan, Suzuken Memorial Foundation, and the Uehara Memorial Foundation. We thank the animal care staff at the Yakult Central Institute, Osamu Ohara, Mayuko Sato, and Kei Hashimoto at RIKEN for their technical supports, Hiroyuki Nobusue and Hideyuki Saya at Keio University for data discussion, and Mamoru Totsuka at the University of Tokyo for providing the aMoS7 cell line.
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/10/8
Y1 - 2015/10/8
N2 - Intestinal Th17 cells are induced and accumulate in response to colonization with a subgroup of intestinal microbes such as segmented filamentous bacteria (SFB) and certain extracellular pathogens. Here, we show that adhesion of microbes to intestinal epithelial cells (ECs) is a critical cue for Th17 induction. Upon monocolonization of germ-free mice or rats with SFB indigenous to mice (M-SFB) or rats (R-SFB), M-SFB and R-SFB showed host-specific adhesion to small intestinal ECs, accompanied by host-specific induction of Th17 cells. Citrobacter rodentium and Escherichia coli O157 triggered similar Th17 responses, whereas adhesion-defective mutants of these microbes failed to do so. Moreover, a mixture of 20 bacterial strains, which were selected and isolated from fecal samples of a patient with ulcerative colitis on the basis of their ability to cause a robust induction of Th17 cells in the mouse colon, also exhibited EC-adhesive characteristics.
AB - Intestinal Th17 cells are induced and accumulate in response to colonization with a subgroup of intestinal microbes such as segmented filamentous bacteria (SFB) and certain extracellular pathogens. Here, we show that adhesion of microbes to intestinal epithelial cells (ECs) is a critical cue for Th17 induction. Upon monocolonization of germ-free mice or rats with SFB indigenous to mice (M-SFB) or rats (R-SFB), M-SFB and R-SFB showed host-specific adhesion to small intestinal ECs, accompanied by host-specific induction of Th17 cells. Citrobacter rodentium and Escherichia coli O157 triggered similar Th17 responses, whereas adhesion-defective mutants of these microbes failed to do so. Moreover, a mixture of 20 bacterial strains, which were selected and isolated from fecal samples of a patient with ulcerative colitis on the basis of their ability to cause a robust induction of Th17 cells in the mouse colon, also exhibited EC-adhesive characteristics.
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U2 - 10.1016/j.cell.2015.08.058
DO - 10.1016/j.cell.2015.08.058
M3 - Article
C2 - 26411289
AN - SCOPUS:84943639694
SN - 0092-8674
VL - 163
SP - 367
EP - 380
JO - Cell
JF - Cell
IS - 2
ER -