Th2 immune response plays a critical role in the development of nickel-induced allergic contact dermatitis

Shiro Niiyama, Hidekazu Tamauchi, Yasuyuki Amoh, Masazumi Terashima, Yukiko Matsumura, Maho Kanoh, Sonoko Habu, Jun Komotori, Kensei Katsuoka

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background:The precise roles of T helper (Th)1-type and Th2-type cytokine responses in nickel (Ni)-induced allergic contact dermatitis have not yet been clearly defined. We investigated the involvement of Th2 cytokines in Ni-induced contact hypersensitivity reaction using GATA-3 transgenic (Tg) mice. Methods: A Ni-titanium (Ti) alloy was implanted under the skin of GATA-3 Tg mice. A Ni solution was then injected 1 month after sensitization. The ear swelling response was measured at several time points after the injection; the cytokine levels in the skin were measured at 48 h after injection, and the serum levels of IgE were measured 1 month after injection. In addition, purified CD4+ splenic cells obtained from the GATA-3 Tg mice sensitized with the Ni-Ti alloy were infused into Rag-2-/- mice, and the ear swelling response of these mice after a further challenge with Ni solution was also measured. Results:Marked ear swelling and elevated serum IgE levels and skin tissue levels of IL-4 were observed in Ni-Ti-sensitized GATA-3 Tg mice. The Rag-2 -/- mice transfused with the CD4+ splenic cells from the Ni-Ti alloy sensitized GATA-3 Tg mice showed a significantly more pronounced ear swelling response than the control mice. Conclusion: We confirmed the participation of Th2-type immune reactions in Ni-induced allergy using GATA-3 Tg mice.

Original languageEnglish
Pages (from-to)303-314
Number of pages12
JournalInternational Archives of Allergy and Immunology
Volume153
Issue number3
DOIs
Publication statusPublished - 2010 Oct 1

Keywords

  • GATA-3
  • Nickel
  • Th2 cytokine
  • Transgenic mice

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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