Th2 immune response plays a critical role in the development of nickel-induced allergic contact dermatitis

Shiro Niiyama, Hidekazu Tamauchi, Yasuyuki Amoh, Masazumi Terashima, Yukiko Matsumura, Maho Kanoh, Sonoko Habu, Jun Komotori, Kensei Katsuoka

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background:The precise roles of T helper (Th)1-type and Th2-type cytokine responses in nickel (Ni)-induced allergic contact dermatitis have not yet been clearly defined. We investigated the involvement of Th2 cytokines in Ni-induced contact hypersensitivity reaction using GATA-3 transgenic (Tg) mice. Methods: A Ni-titanium (Ti) alloy was implanted under the skin of GATA-3 Tg mice. A Ni solution was then injected 1 month after sensitization. The ear swelling response was measured at several time points after the injection; the cytokine levels in the skin were measured at 48 h after injection, and the serum levels of IgE were measured 1 month after injection. In addition, purified CD4+ splenic cells obtained from the GATA-3 Tg mice sensitized with the Ni-Ti alloy were infused into Rag-2-/- mice, and the ear swelling response of these mice after a further challenge with Ni solution was also measured. Results:Marked ear swelling and elevated serum IgE levels and skin tissue levels of IL-4 were observed in Ni-Ti-sensitized GATA-3 Tg mice. The Rag-2 -/- mice transfused with the CD4+ splenic cells from the Ni-Ti alloy sensitized GATA-3 Tg mice showed a significantly more pronounced ear swelling response than the control mice. Conclusion: We confirmed the participation of Th2-type immune reactions in Ni-induced allergy using GATA-3 Tg mice.

Original languageEnglish
Pages (from-to)303-314
Number of pages12
JournalInternational Archives of Allergy and Immunology
Volume153
Issue number3
DOIs
Publication statusPublished - 2010 Oct

Fingerprint

Allergic Contact Dermatitis
Nickel
Transgenic Mice
Ear
Cytokines
Skin
Immunoglobulin E
Injections
Contact Dermatitis
Serum
Interleukin-4
Hypersensitivity
nitinol

Keywords

  • GATA-3
  • Nickel
  • Th2 cytokine
  • Transgenic mice

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Th2 immune response plays a critical role in the development of nickel-induced allergic contact dermatitis. / Niiyama, Shiro; Tamauchi, Hidekazu; Amoh, Yasuyuki; Terashima, Masazumi; Matsumura, Yukiko; Kanoh, Maho; Habu, Sonoko; Komotori, Jun; Katsuoka, Kensei.

In: International Archives of Allergy and Immunology, Vol. 153, No. 3, 10.2010, p. 303-314.

Research output: Contribution to journalArticle

Niiyama, S, Tamauchi, H, Amoh, Y, Terashima, M, Matsumura, Y, Kanoh, M, Habu, S, Komotori, J & Katsuoka, K 2010, 'Th2 immune response plays a critical role in the development of nickel-induced allergic contact dermatitis', International Archives of Allergy and Immunology, vol. 153, no. 3, pp. 303-314. https://doi.org/10.1159/000314372
Niiyama, Shiro ; Tamauchi, Hidekazu ; Amoh, Yasuyuki ; Terashima, Masazumi ; Matsumura, Yukiko ; Kanoh, Maho ; Habu, Sonoko ; Komotori, Jun ; Katsuoka, Kensei. / Th2 immune response plays a critical role in the development of nickel-induced allergic contact dermatitis. In: International Archives of Allergy and Immunology. 2010 ; Vol. 153, No. 3. pp. 303-314.
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