The absence of ribonuclease H1 or H2 alters the sensitivity of Saccharomyces cerevisiae to hydroxyurea, caffeine and ethyl methanesulphonate

Implications for roles of RNases H in DNA replication and repair

A. Arudchandran, S. M. Cerritelli, S. K. Narimatsu, Mitsuhiro Itaya, D. Y. Shin, Y. Shimada, R. J. Crouch

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Background: RNA of RNA-DNA hybrids can be degraded by ribonucleases H present in all organisms including the eukaryote Saccharomyces cerevisiae. Determination of the number and roles of the RNases H in eukaryotes is quite feasible in S. cerevisiae. Results: Two S. cerevisiae RNases H, related to Escherichia coli RNase HI and HII, are not required for growth under normal conditions, yet, compared with wild-type cells, a double-deletion strain has an increased sensitivity to hydroxyurea (HU) and is hypersensitive to caffeine and ethyl methanesulphonate (EMS). In the absence of RNase H1, RNase H2 activity increases, and cells are sensitive to EMS but not HU and are more tolerant of caffeine; the latter requires RNase H2 activity. Cells missing only RNase H2 exhibit increased sensitive to HU and EMS but not caffeine. Conclusions: Mutant phenotypes infer that some RNA-DNA hybrids are recognized by both RNases H1 and H2, while other hybrids appear to be recognized only by RNase H2. Undegraded RNA-DNA hybrids have an effect when DNA synthesis is impaired, DNA damage occurs or the cell cycle is perturbed by exposure to caffeine suggesting a role in DNA replication/repair that can be either beneficial or detrimental to cell viability.

Original languageEnglish
Pages (from-to)789-802
Number of pages14
JournalGenes to Cells
Volume5
Issue number10
DOIs
Publication statusPublished - 2000
Externally publishedYes

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Ethyl Methanesulfonate
Ribonuclease H
Hydroxyurea
Ribonucleases
Caffeine
DNA Replication
DNA Repair
Saccharomyces cerevisiae
RNA
DNA
Eukaryota
DNA Damage
Cell Survival
Cell Cycle
Escherichia coli
Phenotype
triplex DNA
ribonuclease HI
Growth

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

The absence of ribonuclease H1 or H2 alters the sensitivity of Saccharomyces cerevisiae to hydroxyurea, caffeine and ethyl methanesulphonate : Implications for roles of RNases H in DNA replication and repair. / Arudchandran, A.; Cerritelli, S. M.; Narimatsu, S. K.; Itaya, Mitsuhiro; Shin, D. Y.; Shimada, Y.; Crouch, R. J.

In: Genes to Cells, Vol. 5, No. 10, 2000, p. 789-802.

Research output: Contribution to journalArticle

Arudchandran, A. ; Cerritelli, S. M. ; Narimatsu, S. K. ; Itaya, Mitsuhiro ; Shin, D. Y. ; Shimada, Y. ; Crouch, R. J. / The absence of ribonuclease H1 or H2 alters the sensitivity of Saccharomyces cerevisiae to hydroxyurea, caffeine and ethyl methanesulphonate : Implications for roles of RNases H in DNA replication and repair. In: Genes to Cells. 2000 ; Vol. 5, No. 10. pp. 789-802.
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AU - Crouch, R. J.

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