The APC/C activator Cdh1 regulates the G2/M transition during differentiation of placental trophoblast stem cells

Hideaki Naoe, Tatsuyuki Chiyoda, Jo Ishizawa, Kenta Masuda, Hideyuki Saya, Shinji Kuninaka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Differentiation of placental trophoblast stem (TS) cells to trophoblast giant (TG) cells is accompanied by transition from a mitotic cell cycle to an endocycle. Here, we report that Cdh1, a regulator of the anaphase-promoting complex/cyclosome (APC/C), negatively regulates mitotic entry upon the mitotic/endocycle transition. TS cells derived from homozygous Cdh1 gene-trapped (Cdh1GT/GT) murine embryos accumulated mitotic cyclins and precociously entered mitosis after induction of TS cell differentiation, indicating that Cdh1 is required for the switch from mitosis to the endocycle. Furthermore, the Cdh1GT/GT TS cells and placenta showed aberrant expression of placental differentiation markers. These data highlight an important role of Cdh1 in the G2/M transition during placental differentiation.

Original languageEnglish
Pages (from-to)757-762
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume430
Issue number2
DOIs
Publication statusPublished - 2013 Jan 11

Keywords

  • APC/C
  • Cdh1
  • Endocycle
  • G2/M transition
  • Trophoblast giant cell
  • Trophoblast stem cell

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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