The application of in vivo confocal scanning laser microscopy in the management of Acanthamoeba keratitis

Yukihiro Matsumoto, Murat Dogru, Enrique Adan Sato, Yasuhiro Katono, Yuichi Uchino, Shigeto Shimmura, Kazuo Tsubota

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Purpose: To evaluate the role of in vivo laser scanning confocal microscopy (Heidelberg Retina Tomograph II, Rostock Cornea Module, HRTII-RCM) in the management of Acanthamoeba keratitis (AK). Methods: Four eyes of four patients with AK seen at Keio University Hospital at the Department of Ophthalmology were studied in this single-center, prospective, interventional case series. All patients were routinely examined by slit-lamp microscopy including corneal fluorescein staining. Best corrected visual acuity (BCVA) was also measured before and after the treatment for AK. Both the scraped corneal epithelium and soft contact lens (SCL) storage solution in each patient's SCL case were cultured. Patient corneas were examined regularly using the HRTII-RCM before treatment and after commencement of medications including azoles, echinocandins, and chlorhexidine. Results: All patients had various degrees of conjunctival injection, corneal edema, stromal opacity with radial keratoneuritis with slit-lamp examination. Cultures for AK were positive in three out of four cases by corneal scraping. Contact lens storage solutions were also positive in three of four cases. HRTII-RCM examination could detect Acanthamoeba cysts or trophozoites in all eyes before corneal scraping. No organisms were detectable in any of the cases in any of the corneal layers four to six weeks after treatment. The BCVA improved with treatment in three of four eyes. Conclusions: HRTII-RCM could effectively demonstrate cysts and trophozoites and the nature of the inflammatory process in AK. In vivo laser scanning confocal microscopy employing HRTII-RCM could provide an end-point for treatment, saving the patient from additional invasive diagnostic procedures and unneeded exposure to long term topical or systemic medications.

Original languageEnglish
Pages (from-to)1319-1326
Number of pages8
JournalMolecular Vision
Volume13
Publication statusPublished - 2007 Jul 25

Fingerprint

Acanthamoeba Keratitis
Confocal Microscopy
Cornea
Retina
Contact Lens Solutions
Hydrophilic Contact Lens
Trophozoites
Visual Acuity
Cysts
Echinocandins
Corneal Edema
Acanthamoeba
Therapeutics
Azoles
Corneal Epithelium
Chlorhexidine
Hospital Departments
Ophthalmology
Fluorescein
Staining and Labeling

ASJC Scopus subject areas

  • Ophthalmology

Cite this

The application of in vivo confocal scanning laser microscopy in the management of Acanthamoeba keratitis. / Matsumoto, Yukihiro; Dogru, Murat; Sato, Enrique Adan; Katono, Yasuhiro; Uchino, Yuichi; Shimmura, Shigeto; Tsubota, Kazuo.

In: Molecular Vision, Vol. 13, 25.07.2007, p. 1319-1326.

Research output: Contribution to journalArticle

@article{e9c371d481aa45d6a08fb35e20b037af,
title = "The application of in vivo confocal scanning laser microscopy in the management of Acanthamoeba keratitis",
abstract = "Purpose: To evaluate the role of in vivo laser scanning confocal microscopy (Heidelberg Retina Tomograph II, Rostock Cornea Module, HRTII-RCM) in the management of Acanthamoeba keratitis (AK). Methods: Four eyes of four patients with AK seen at Keio University Hospital at the Department of Ophthalmology were studied in this single-center, prospective, interventional case series. All patients were routinely examined by slit-lamp microscopy including corneal fluorescein staining. Best corrected visual acuity (BCVA) was also measured before and after the treatment for AK. Both the scraped corneal epithelium and soft contact lens (SCL) storage solution in each patient's SCL case were cultured. Patient corneas were examined regularly using the HRTII-RCM before treatment and after commencement of medications including azoles, echinocandins, and chlorhexidine. Results: All patients had various degrees of conjunctival injection, corneal edema, stromal opacity with radial keratoneuritis with slit-lamp examination. Cultures for AK were positive in three out of four cases by corneal scraping. Contact lens storage solutions were also positive in three of four cases. HRTII-RCM examination could detect Acanthamoeba cysts or trophozoites in all eyes before corneal scraping. No organisms were detectable in any of the cases in any of the corneal layers four to six weeks after treatment. The BCVA improved with treatment in three of four eyes. Conclusions: HRTII-RCM could effectively demonstrate cysts and trophozoites and the nature of the inflammatory process in AK. In vivo laser scanning confocal microscopy employing HRTII-RCM could provide an end-point for treatment, saving the patient from additional invasive diagnostic procedures and unneeded exposure to long term topical or systemic medications.",
author = "Yukihiro Matsumoto and Murat Dogru and Sato, {Enrique Adan} and Yasuhiro Katono and Yuichi Uchino and Shigeto Shimmura and Kazuo Tsubota",
year = "2007",
month = "7",
day = "25",
language = "English",
volume = "13",
pages = "1319--1326",
journal = "Molecular Vision",
issn = "1090-0535",

}

TY - JOUR

T1 - The application of in vivo confocal scanning laser microscopy in the management of Acanthamoeba keratitis

AU - Matsumoto, Yukihiro

AU - Dogru, Murat

AU - Sato, Enrique Adan

AU - Katono, Yasuhiro

AU - Uchino, Yuichi

AU - Shimmura, Shigeto

AU - Tsubota, Kazuo

PY - 2007/7/25

Y1 - 2007/7/25

N2 - Purpose: To evaluate the role of in vivo laser scanning confocal microscopy (Heidelberg Retina Tomograph II, Rostock Cornea Module, HRTII-RCM) in the management of Acanthamoeba keratitis (AK). Methods: Four eyes of four patients with AK seen at Keio University Hospital at the Department of Ophthalmology were studied in this single-center, prospective, interventional case series. All patients were routinely examined by slit-lamp microscopy including corneal fluorescein staining. Best corrected visual acuity (BCVA) was also measured before and after the treatment for AK. Both the scraped corneal epithelium and soft contact lens (SCL) storage solution in each patient's SCL case were cultured. Patient corneas were examined regularly using the HRTII-RCM before treatment and after commencement of medications including azoles, echinocandins, and chlorhexidine. Results: All patients had various degrees of conjunctival injection, corneal edema, stromal opacity with radial keratoneuritis with slit-lamp examination. Cultures for AK were positive in three out of four cases by corneal scraping. Contact lens storage solutions were also positive in three of four cases. HRTII-RCM examination could detect Acanthamoeba cysts or trophozoites in all eyes before corneal scraping. No organisms were detectable in any of the cases in any of the corneal layers four to six weeks after treatment. The BCVA improved with treatment in three of four eyes. Conclusions: HRTII-RCM could effectively demonstrate cysts and trophozoites and the nature of the inflammatory process in AK. In vivo laser scanning confocal microscopy employing HRTII-RCM could provide an end-point for treatment, saving the patient from additional invasive diagnostic procedures and unneeded exposure to long term topical or systemic medications.

AB - Purpose: To evaluate the role of in vivo laser scanning confocal microscopy (Heidelberg Retina Tomograph II, Rostock Cornea Module, HRTII-RCM) in the management of Acanthamoeba keratitis (AK). Methods: Four eyes of four patients with AK seen at Keio University Hospital at the Department of Ophthalmology were studied in this single-center, prospective, interventional case series. All patients were routinely examined by slit-lamp microscopy including corneal fluorescein staining. Best corrected visual acuity (BCVA) was also measured before and after the treatment for AK. Both the scraped corneal epithelium and soft contact lens (SCL) storage solution in each patient's SCL case were cultured. Patient corneas were examined regularly using the HRTII-RCM before treatment and after commencement of medications including azoles, echinocandins, and chlorhexidine. Results: All patients had various degrees of conjunctival injection, corneal edema, stromal opacity with radial keratoneuritis with slit-lamp examination. Cultures for AK were positive in three out of four cases by corneal scraping. Contact lens storage solutions were also positive in three of four cases. HRTII-RCM examination could detect Acanthamoeba cysts or trophozoites in all eyes before corneal scraping. No organisms were detectable in any of the cases in any of the corneal layers four to six weeks after treatment. The BCVA improved with treatment in three of four eyes. Conclusions: HRTII-RCM could effectively demonstrate cysts and trophozoites and the nature of the inflammatory process in AK. In vivo laser scanning confocal microscopy employing HRTII-RCM could provide an end-point for treatment, saving the patient from additional invasive diagnostic procedures and unneeded exposure to long term topical or systemic medications.

UR - http://www.scopus.com/inward/record.url?scp=34547530825&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547530825&partnerID=8YFLogxK

M3 - Article

VL - 13

SP - 1319

EP - 1326

JO - Molecular Vision

JF - Molecular Vision

SN - 1090-0535

ER -